Water Extract of Mentha × villosa: Phenolic Fingerprint and Effect on Ischemia-Reperfusion Injury

Abstract

Qualitative analysis of the water extract of Mentha x villosa Huds. leaves was performed by liquid chromatography mass spectrometry (LC-MS/MS) and quantitative analysis was made by reverse-phase liquid chromatography coupled with photodiode array detection (LC-DAD). Sixteen phenolic compounds were identified and quantified consisting of 8 phenolic acids/derivatives and 8 flavonoid glycosides (quinic acid, chlorogenic acid, coumaroyl-hexoside, caffeic acid, coumaroylquinic acid, lithospermic acid, rosmarinic acid, salvianolic acid A, luteolin-7- O-glucuronide, luteolin-7- O-glucoside, luteolin-7- O-rutinoside, eriodictyol-7- O-rutinoside, apigenin-7- O-glucuronide, kaempferol-3- O-glucuronide, chrysoeriol-7- O-rutinoside, and hesperetin-7- O-rutinoside). Luteolin-7- O-rutinoside (25.6 ± 0.7 mg/g dry extract) and rosmarinic acid (17.9 ± 0.4 mg/g dry extract) were the most abundant. High antioxidant activity of this phenolic-rich water extract was confirmed in vitro by DPPH and ABTS tests and ex vivo in the ischemia-reperfusion injured rat superior mesenteric artery. Thus, the water extract of M. x villosa leaves seems to be a promising agent in prevention of tissue injury caused by oxidative stress.

Mentha spicata L. infusions as sources of antioxidant phenolic compounds: emerging reserve lots with special harvest requirements

“…Compound 13 presented a pseudomolecular ion [M − H] − at m/z 607, yielding fragment ions at m/z 299 (−308 u; loss of a deoxyhexosyl-hexoside residue), which allowed its assignment as chrysoeriol-7-O-rutinoside, taking into account its previous identification in another Mentha species, such as Mentha × villosa. 21 The presence of luteolin-7-O-glucoside and luteolin has been reported in M. spicata, 7,8,13,14,22 although, to the best of our knowledge, the two compounds detected herein have not been reported in M. spicata.…”

Phenolic Compounds and Biological Activity of Selected Mentha Species

“…In general, the results presented here were in agreement with those found in the literature ( Table 1 ). RA is the main phenolic acid found in this genus [ 1 , 12 , 32 , 54 ], followed by the flavone LUT [ 1 , 12 , 27 , 57 ] and the flavanone eriocitrin [ 1 , 34 , 46 ]. The levels of phenolic compounds, as well as volatile terpenoids, are dependent on the phenological stage of the plant.…”

Biophenols of mints: Antioxidant, acetylcholinesterase, butyrylcholinesterase and histone deacetylase inhibition activities targeting Alzheimer’s disease treatment

“…d Previously reported by Hawrył (2014), Dorman et al (2003) and Ferreira, Pereira, Cardoso, Oliveira, and Moreno (2014) in M. aquatica. e Previously reported by Moldovan et al (2014), Bichra, El-Modafar, El-Abbassi, Bouamama, and Benkhalti (2013) and Fialovaa et al (2015) in M. suaveolens, its variegated form or hybrids.…”