Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor

Zhang, Xia; Wollenweber, Bernd; Jiang, Dong; Liu, Fulai; Zhao, Jun

doi:10.1093/jxb/erm364

Cited by 147 publications

(84 citation statements)

References 63 publications

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“…Contents of green plant pigments including carotenoids and their ratios are good indicators of stress detection and tolerance in plants. 15 Analysis of variance showed the significant differences between the groups in parameters studied. It has been reported that the terminal heat stress imposes complicate the problems for harvesting higher yield.…”

Section: Discussionmentioning

confidence: 92%

Comparative physiological response of wheat genotypes under terminal heat stress

Dhyani

Ansari

Rao

et al. 2013

Plant Signaling & Behavior

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Keywords: heat stress, malondialdehyde, proline, superoxide dismutase, timely and late sown condition, wheat genotypes thermostability, canopy function, stable green habit, better mobilization rate of food reserve and other technical or physiological parameters might be helpful to overcome yield loss under terminal heat stress. 12 In vitro studies of wheat plants in combination with genetic manipulation to develop heat tolerant wheat are of limited success. Therefore, the complex physiological-genetic approach could be useful to acquire heat tolerance in wheat to minimize the farmer's risk for reduced yield and low quality grain product. Here, Physiological evaluation of wheat genotypes was performed under timely and late sown condition. Results Estimation of chlorophyll index (SPAD-values), leaf area index (LAI) and proline content. Chlorophyll content (SPAD-values)of wheat genotypes viz., DBW-140, RAJ-3765, PBW-574, K-0-307 and HS-240 under timely and late sown was calculated after 30, 50, 70, 90 and 110 d. It was found that SPAD-values affected under timely and late sown conditions were 41.5, 38.9, 37.08, 37.67, 47.7, 47.59, 46.56, 46.73, 41.03, 40 and 40.15, 34.5, 34.76, 33.33, 45.63, 44, 43.03, 42.52, 4,137 and HS-240 genotypes showed highest reduction in LAI (0.3, 1.36, 3.78, 3.0 and 0.37, 2.08, 3.94, 3.29) at 30, 70 and 90 d after sowing, respectively (Fig. 1B). The proline content (μg g −1 FW) was found to be increased in all the genotypes studied (30.25, 160.5, 135.2, 262.1, 184.0 and 221.68, 236.55, 261.77, 419.35) under late sown condition as compared with that of timely sown condition (89.6, 106.8, 119.6, 62.0 and 21.98, 20.66, 37.0, 20.86, 18.37) at both anthesis and 15 DAS, except , respectively (Fig. 1C).Evaluation of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, heat susceptibility index (s) (HIS) and yield associated traits. The amount of malondialdehyde (MDA) (μ mole g −1 FW) produced when polyunsaturated fatty acids in the membrane undergone lipid peroxidation in wheat genotypes were calculated as 13. 06, 11.80 and 7.26, 4.23 in HS 240 and K-0-307 genotypes respectively at the time of anthesis in late sown and timely sown conditions. The similar trends were also observed in HS 240 and K-0-307 genotypes 15 DAS, which followed by Raj 3765, DBW 140 and PBW 574 genotypes ( Fig. 2A). There is no significant increase in superoxide dismutase (SOD) activity (unit mg −1 FW) in genotypes evaluated at anthesis stage under timely and late sown conditions. In contrast, after 15 d of anthesis SOD activity was highest in RAJ-3765 (1.88) whereas in HS-240 and K-0-307 genotypes it was lowest (1.63 and 1.60) under late sown condition (Fig. 2B). The heat susceptibility index (HIS) of HS 240 and K-0-307 wheat genotypes was highest (1.33, 1.57, 1.51 and 1.17, 1.34, 1.65), which followed by DBW 140, Raj 3765 and PBW 574 genotypes (0.846, 0.649 and 0.411), (0.780, 0.905 and 0.905) and (0.897, 0.457 abd 0.682) for 1000-grain weight, grain weight and grain yield, respectively (Fig. 2C). Y...

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Section: Discussionmentioning

confidence: 92%

Comparative physiological response of wheat genotypes under terminal heat stress

Dhyani

Ansari

Rao

et al. 2013

Plant Signaling & Behavior

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“…The supernatant was quantified through monitoring the absorbance at 663 and 645 nm using a spectrophotometer (Zhang et al, 2008 For electrolyte leakage measurement, seeds were sown on soil and grown in a chamber at 22°C under a 16-h photoperiod. Four-week-old plantlets were measured according to described methods (Clarke et al, 2004) with some modifications.…”

Section: Determination Of Chlorophyll and Electrolyte Leakage Measurmentioning

confidence: 99%

Proline Accumulation Is Inhibitory to Arabidopsis Seedlings during Heat Stress

et al. 2011

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The effect of proline (Pro) accumulation on heat sensitivity was investigated using transgenic Arabidopsis (Arabidopsis thaliana) plants ectopically expressing the D(1)-pyrroline-5-carboxylate synthetase 1 gene (AtP5CS1) under the control of a heat shock protein 17.6II gene promoter. During heat stress, the heat-inducible expression of the AtP5CS1 transgene was capable of enhancing Pro biosynthesis. Twelve-day-old seedlings were first treated with heat at 37°C for 24 h to induce Pro and then were stressed at 50°C for 4 h. After recovery at 22°C for 96 h, the growth of Pro-overproducing plants was significantly more inhibited than that of control plants that do not accumulate Pro, manifested by lower survival rate, higher ion leakage, higher reactive oxygen species (ROS) and malondialdehyde levels, and increased activity of the Pro/P5C cycle. The activities of antioxidant enzymes superoxide dismutase, guaiacol peroxidase, and catalase, but not those of glutathione reductase and ascorbate peroxidase, increased in all lines after heat treatment, but the increase was more significant in Pro-overproducing seedlings. Staining with MitoSox-Red, reported for being able to specifically detect superoxide formed in mitochondria, showed that Pro accumulation during heat stress resulted in elevated levels of ROS in mitochondria. Interestingly, exogenous abscisic acid (ABA) and ethylene were found to partially rescue the heat-sensitive phenotype of Pro-overproducing seedlings. Measurement of ethylene and ABA levels further confirmed that these two hormones are negatively affected in Pro-overproducing seedlings during heat stress. Our results indicated that Pro accumulation under heat stress decreases the thermotolerance, probably by increased ROS production via the Pro/P5C cycle and inhibition of ABA and ethylene biosynthesis.

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“…A number of stress-related genes in maize have been identified and characterized well. However, only a few of maize bZIP transcription factor genes related to abiotic stress were reported (Nieva et al 2005;Zhang et al 2008Zhang et al , 2011Jia et al 2009). In present study, we isolated a putative bZIP gene, ZmbZIP72, analyzed its expression profile under different treatments, and characterized its role in stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis

Ying

Zhang

et al. 2011

Planta

188

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In plants, the bZIP (basic leucine zipper) transcription factors regulate diverse functions, including processes such as plant development and stress response. However, few have been functionally characterized in maize (Zea mays). In this study, we cloned ZmbZIP72, a bZIP transcription factor gene from maize, which had only one copy in the maize genome and harbored three introns. Analysis of the amino acid sequence of ZmbZIP72 revealed a highly conserved bZIP DNA-binding domain in its C-terminal region, and four conserved sequences distributed in N- or C-terminal region. The ZmbZIP72 gene expressed differentially in various organs of maize plants and was induced by abscisic acid, high salinity, and drought treatment in seedlings. Subcellular localization analysis in onion epidermal cells indicated that ZmbZIP72 was a nuclear protein. Transactivation assay in yeast demonstrated that ZmbZIP72 functioned as a transcriptional activator and its N terminus (amino acids 23-63) was necessary for the transactivation activity. Heterologous overexpression of ZmbZIP72 improved drought and partial salt tolerance of transgenic Arabidopsis plants, as determined by physiological analyses of leaf water loss, electrolyte leakage, proline content, and survival rate under stress. In addition, the seeds of ZmbZIP72-overexpressing transgenic plants were hypersensitive to ABA and osmotic stress. Moreover, overexpression of ZmbZIP72 enhanced the expression of ABA-inducible genes such as RD29B, RAB18, and HIS1-3. These results suggest that the ZmbZIP72 protein functions as an ABA-dependent transcription factor in positive modulation of abiotic stress tolerance and may be a candidate gene with potential application in molecular breeding to enhance stress tolerance in crops.

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Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor

Cited by 147 publications

References 63 publications

Comparative physiological response of wheat genotypes under terminal heat stress

Comparative physiological response of wheat genotypes under terminal heat stress

Proline Accumulation Is Inhibitory to Arabidopsis Seedlings during Heat Stress

Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis

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