Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system

Kammerloher, Werner; Fischer, Utz; Piechottka, Gerd P.; Schäffner, Anton R.

doi:10.1046/j.1365-313x.1994.6020187.x

Cited by 316 publications

(280 citation statements)

References 37 publications

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“…OsPIP1;2, OsPIP1;3, OsPIP2;1, OsPIP2;3, OsPIP2;4, OsPIP2;5 and OsPIP2;6, were transcribed at higher rates in roots than leaves and only one PIP gene, OsPIP2;7, followed the pattern of mRNA accumulation of higher levels in leaves than roots. Inconsistency between aquaporin expression at the mRNA level and protein accumulation was also noted in a previous study [8,30]. These authors postulated that post-transcriptional mechanisms resulted in this, e.g.…”

Section: Pip Expression In Upland Rice and Lowland Rice Under Normalsupporting

confidence: 63%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins (PIPs), was studied at both the protein and mRNA level in upland rice (Oryza sativa L. cv. Zhonghan 3) and lowland rice (Oryza sativa L. cv. Xiushui 63) when they were water stressed by treatment with 20% polyethylene glycol (PEG). Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1;2, Os-PIP1;3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1;2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid (ABA) level in upland rice but not in lowland rice under water deficit. Treatment with 60 µM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling pathways during water deficit.

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Section: Pip Expression In Upland Rice and Lowland Rice Under Normalsupporting

confidence: 63%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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“…Silencing water channel expression seems to reduce the ability of seeds to absorb water as a result of the water channel deficiency. However, PIP1 isoforms have often been found to have very low, or no, water permeability when expressed solely in Xenopus oocytes [22,[38][39][40]. Previously, Chaumont and his co-workers [41] have demonstrated that interactions between maize ZmPIP1;1 and ZmPIP1;2 or between ZmPIP1;2 and ZmPIP2 positively modulated their water channel activity.…”

Section: Discussionmentioning

confidence: 99%

The role of water channel proteins and nitric oxide signaling in rice seed germination

et al. 2007

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Previous studies have demonstrated the possible role of several aquaporins in seed germination. But systematic investigation of the role of aquaporin family members in this process is lacking. Here, the developmental regulation of plasma membrane intrinsic protein (PIP) expression throughout germination and post-germination processes in rice embryos was analyzed. The expression patterns of the PIPs suggest these aquaporins play different roles in seed germination and seedling growth. Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions. Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination. Our data also revealed that the nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), promoted seed germination; furthermore, the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, inhibited germination and reduced the stimulative effects of SNP and GSNO on rice germination. Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds. These results suggest that water channels play an important role in seed germination, acting, at least partly, in response to the NO signaling pathway.

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“…Osmotic water permeability measurements have been used in Xenopus oocytes for the identification of functional aquaporins (Preston et al, 1992 ;Maurel et al, 1993 ;Daniels et al, 1994 ;Karmmerloher et al, 1994). For such large cells, the permeability time is so long that the mixing time (when the osmotic shock occurs) can usually be neglected.…”

Section: mentioning

confidence: 99%

Does calcium ameliorate the negative effect of NaCl on melon root water transport by regulating aquaporin activity?

2000

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The hydraulic conductance (L ! ) of detached, exuding root systems from melon (Cucumis melo cv. Amarillo oro) was measured. All plants received a half-strength Hoagland nutrient solution, and plants stressed either solely with NaCl (50 mM) or with NaCl (50 mM) following treatment (2 d) with CaCl # (10 mM) were compared with controls and CaCl # -treated (10 mM) plants. The L ! of NaCl-treated plants was markedly decreased when compared to control and CaCl # -treated plants, but the decrease was smaller when NaCl was added to plants previously treated with CaCl # . A similar effect was observed when the flux of Ca# + into the xylem and the Ca# + concentration in the plasma membrane of the root cells were determined. In control, CaCl # -and NaCljCaCl # -treated plants, HgCl # treatment (50 µM) caused a sharp decline in L ! to values similar to those of NaCl-stressed roots, but L ! was restored by treatment with 5 mM DTT. However, in NaCl roots only a slight effect of Hg# + and DTT were observed. The effect of all treatments on L ! was similar to that on osmotic water permeability (P f ) of individual protoplasts isolated from roots. The results suggest that NaCl decreased the passage of water through the membrane and roots by reducing the activity of Hg-sensitive water channels. The ameliorative effect of Ca# + on NaCl stress could be related to water-channel function.

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Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system

Cited by 316 publications

References 37 publications

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

The role of water channel proteins and nitric oxide signaling in rice seed germination

Does calcium ameliorate the negative effect of NaCl on melon root water transport by regulating aquaporin activity?

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