Does calcium ameliorate the negative effect of NaCl on melon root water transport by regulating aquaporin activity?

Carvajal, Micaela; Cerdà, Artemi; Martı́nez, Vicente

doi:10.1046/j.1469-8137.2000.00593.x

Cited by 123 publications

(64 citation statements)

References 50 publications

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“…Okadaic acid and vanadate are commonly used as inhibitors of protein phosphorylation (Cohen and Cohen, 1989;Gordon, 1991). We have also demonstrated that the calcium channel blocker, 1 mM LaCl 3 , significantly inhibited L p at 25°C, further supporting the notion that aquaporin gating is linked to the calcium signal (Johansson et al, 1996;Carvajal et al, 2000;Németh-Cahalan and Hall, 2000;Azad et al, 2004).…”

Section: Discussionsupporting

confidence: 76%

Overexpression of PIP2;5 Aquaporin Alleviates Effects of Low Root Temperature on Cell Hydraulic Conductivity and Growth in Arabidopsis

et al. 2012

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The effects of low root temperature on growth and root cell water transport were compared between wild-type Arabidopsis (Arabidopsis thaliana) and plants overexpressing plasma membrane intrinsic protein 1;4 (PIP1;4) and PIP2;5. Descending root temperature from 25°C to 10°C quickly reduced cell hydraulic conductivity (L p ) in wild-type plants but did not affect L p in plants overexpressing PIP1;4 and PIP2;5. Similarly, when the roots of wild-type plants were exposed to 10°C for 1 d, L p was lower compared with 25°C. However, there was no effect of low root temperature on L p in PIP1;4-and PIP2;5-overexpressing plants after 1 d of treatment. When the roots were exposed to 10°C for 5 d, L p was reduced in wild-type plants and in plants overexpressing PIP1;4, whereas there was still no effect in PIP2;5-overexpressing plants. These results suggest that the gating mechanism in PIP1;4 may be more sensitive to prolonged low temperature compared with PIP2;5. The reduction of L p at 10°C in roots of wild-type plants was partly restored to the preexposure level by 5 mM Ca(NO 3 ) 2 and protein phosphatase inhibitors (75 nM okadaic acid or 1 mM Na 3 VO 4 ), suggesting that aquaporin phosphorylation/dephosphorylation processes were involved in this response. The temperature sensitivity of cell water transport in roots was reflected by a reduction in shoot and root growth rates in the wild-type and PIP1;4-overexpressing plants exposed to 10°C root temperature for 5 d. However, low root temperature had no effect on growth in plants overexpressing PIP2;5. These results provide strong evidence for a link between growth at low root temperature and aquaporin-mediated root water transport in Arabidopsis.

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Section: Discussionsupporting

confidence: 76%

Overexpression of PIP2;5 Aquaporin Alleviates Effects of Low Root Temperature on Cell Hydraulic Conductivity and Growth in Arabidopsis

et al. 2012

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“…Exogenous ALA application remarkably reduced the damage caused by salinity stress and improved plant biomass accumulation in both tomato cultivars, which is consistent with previous research on cotton (Watanabe et al, 2000) and potato (Zhang et al, 2006). Salinity stress decreases osmotic pressure dependent hydraulic conductance in melon roots (Carvajal et al, 2000). Likewise, root hydraulic conductance and E in citrus were remarkably reduced by long-term salt treatments (Rodriguez-Gamir et al, 2012).…”

Section: Discussionmentioning

confidence: 92%

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Zhao

Yan

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The effects of exogenous 5-aminolevulinic acid (ALA) on photosynthesis, plant growth, and the expression of two aquaporin genes in tomato seedlings under control and salinity conditions were investigated. Exogenous ALA application significantly improved net photosynthetic rate (P n ), total chlorophyll content, and plant biomass accumulation of tomato seedlings under salinity stress. As revealed by real-time PCR analyses, after treatment with ALA alone, expression of both LePIP1 and LePIP2 in the two tomato cultivars was upregulated at 2 h and subsequently decreased to normal levels. Under salinity stress, transcript levels of LePIP1 in both leaves and roots of salt-sensitive cultivars (cv. Zhongza No.9) increased significantly and were considerably higher than in cultivars exposed to ALA alone. In contrast, the expression levels of LePIP1 and LePIP2 in cvs. Jinpeng No.1 cultivars were slightly lower under salinity stress than under ALA treatment. In addition, transcript levels of both LePIP1 and LePIP2 in the roots of Jinpeng No. 1 cultivars were considerably lower than those Y.Y. Zhao et al. 6402©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6401-6412 (2015) in the roots of Zhongza No. 9 cultivars under salinity stress, regardless of ALA supplementation, implying that Jinpeng No. 1 cultivars had a better capacity to maintain membrane intrinsic protein stability. Further, ALA application distinctly counteracted the up-or down-regulation of LePIP1 and LePIP2 in both cultivars under salinity stress, in accordance with the improvements instomatal conductance, transpiration rate, and P n of tomato leaves. The results presented here indicate that ALA controls aquaporin expression, thus, presumably ALA regulates water homeostasis and enhances salt tolerance of tomato seedlings.

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“…In ice plants, transcript levels of some aquaporins are down-regulated in the first 30 h after exposure to salt stress and recover when this stress is interrupted (Yamada et al, 1995). Likewise, NaCl reduces either the activity or abundance of Hg-sensitive water channels (aquaporins) as shown by measuring the osmotic pressuredependent hydraulic conductivity of detached, exuding root systems from paprika pepper (Carvajal et al, 1999) and from melon (Carvajal et al, 2000). In line with these observations, the amount of LeTIP and LePIP1 but not of LePIP2 transcripts was found to be down-regulated by roughly 20% under continuous salt stress in the present communication (Fig.…”

Section: Discussionmentioning

confidence: 99%

Analysis of expression of aquaporins and Na+/H+ transporters in tomato colonized by arbuscular mycorrhizal fungi and affected by salt stress

Ouziad

Wilde

Schmelzer³

et al. 2006

Environmental and Experimental Botany

132

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Among the proteins functioning in salt tolerance of plants, Na + /H + transporters and aquaporins appear to be of paramount importance. The present study compares expression of Na + /H + transporter and aquaporin genes in tomato colonized by arbuscular mycorrhizal fungi (AMF) and in non-colonized controls under NaCl stress. As revealed by Northern analyses and in situ hybridizations, expression of two Na + /H + transporter genes is not significantly affected by salt stress or by colonization of the plants with AMF. In contrast, transcript levels of both a tonoplast and a plasmalemma aquaporin gene are reduced by salt stress, and this effect is distinctly enhanced by colonization of the tomato roots with AMF. In leaves, colonization of tomato by AMF results in a drastic increase of the mRNA of all three aquaporin genes assayed under salt stress. Aquaporins are known to significantly contribute to water movement in plants. The results presented here indicate that AMF controls aquaporin expression and thereby presumably regulates water flow in tomato under salt stress.

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Does calcium ameliorate the negative effect of NaCl on melon root water transport by regulating aquaporin activity?

Cited by 123 publications

References 50 publications

Overexpression of PIP2;5 Aquaporin Alleviates Effects of Low Root Temperature on Cell Hydraulic Conductivity and Growth in Arabidopsis

Overexpression of PIP2;5 Aquaporin Alleviates Effects of Low Root Temperature on Cell Hydraulic Conductivity and Growth in Arabidopsis

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Analysis of expression of aquaporins and Na+/H+ transporters in tomato colonized by arbuscular mycorrhizal fungi and affected by salt stress

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