Water-based preparation of spider silk films as drug delivery matrices

Agostini, Elisa; Winter, Gerhard; Engert, Julia

doi:10.1016/j.jconrel.2015.06.025

Cited by 27 publications

(48 citation statements)

References 41 publications

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“…Recombinant scaffold proteins produced from plant hosts promoted cell attachment and proliferation (Stein et al, 2009;Hauptmann et al, 2015). With the known affinity of spider silk analogues for liposome binding and encapsulation of low molecular weight compounds (Antonenko et al, 2010;Hardy et al, 2013;Agostini et al, 2015), plant derived spider silk proteins may also be used for controllable intake and release of therapeutic molecules (Doblhofer and Scheibel, 2015). The special affinity of spider silk mimics to HER2 expressing cells (Florczak et al, 2014) and the tumor-homing peptides (Numata et al, 2011) provides opportunities for the spider silk-based materials to be used in targeted melanoma cell delivery.…”

Section: Potential Application Of Scaffold Proteins For Drug Deliverymentioning

confidence: 99%

Advances in Plant-Derived Scaffold Proteins

2020

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Scaffold proteins form critical biomatrices that support cell adhesion and proliferation for regenerative medicine and drug screening. The increasing demand for such applications urges solutions for cost effective and sustainable supplies of hypoallergenic and biocompatible scaffold proteins. Here, we summarize recent efforts in obtaining plantderived biosynthetic spider silk analogue and the extracellular matrix protein, collagen. Both proteins are composed of a large number of tandem block repeats, which makes production in bacterial hosts challenging. Furthermore, post-translational modification of collagen is essential for its function which requires co-transformation of multiple copies of human prolyl 4-hydroxylase. We discuss our perspectives on how the GAANTRY system could potentially assist the production of native-sized spider dragline silk proteins and prolyl hydroxylated collagen. The potential of recombinant scaffold proteins in drug delivery and drug discovery is also addressed.

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Section: Potential Application Of Scaffold Proteins For Drug Deliverymentioning

confidence: 99%

Advances in Plant-Derived Scaffold Proteins

2020

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“…Other advantages of using drug delivery systems are to increase the loading efficiency of drugs which have low water solubility, and increase control over the release profile of the pharmaceutical agent . Simply stated, drug delivery systems allow for a reduction in the amount of drug administered, frequency of drug administration, as well as the potential side effects of a drug …”

Section: Drug Deliverymentioning

confidence: 99%

“…In both cases, films were formed from eADF4(C16) by film casting. In order to allow an easy removal, films were cast onto a polytetrafluorethylene surface either from organic HFIP or aqueous solution . In the study conducted by Hardy et al, eADF4(C16) was additionally blended with polycaprolactone and thermoplastic polyurethane at various ratios, and resulting films were about 100 µm thick .…”

Section: Drug Deliverymentioning

confidence: 99%

“…In the study conducted by Hardy et al, eADF4(C16) was additionally blended with polycaprolactone and thermoplastic polyurethane at various ratios, and resulting films were about 100 µm thick . The films were well‐characterized using differential scanning calorimetry and tensile testing (Young's moduli with 5500 or 3300 MPa), as well as thermogravimetric analysis, isoelectric point, water absorption, and water contact angle measurements.…”

Section: Drug Deliverymentioning

confidence: 99%

“…Multilayer films prepared by pressing a FITC–BSA and glycerol (glue) film between two pure silk films did not show any improvement in terms of release. Coating of FITC–BSA and 2‐pyrrolidone (plasticizer) loaded films lead to a decrease in burst release to 20% followed by a steady release over 90 d …”

Section: Drug Deliverymentioning

confidence: 99%

See 2 more Smart Citations

Biomedical Applications of Recombinant Silk‐Based Materials

Aigner

DeSimone

Scheibel³

2018

Advanced Materials

240

203

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Silk is mostly known as a luxurious textile, which originates from silkworms first cultivated in China. A deeper look into the variety of silk reveals that it can be used for much more, in nature and by humanity. For medical purposes, natural silks were recognized early as a potential biomaterial for surgical threads or wound dressings; however, as biomedical engineering advances, the demand for high-performance, naturally derived biomaterials becomes more pressing and stringent. A common problem of natural materials is their large batch-to-batch variation, the quantity available, their potentially high immunogenicity, and their fast biodegradation. Some of these common problems also apply to silk; therefore, recombinant approaches for producing silk proteins have been developed. There are several research groups which study and utilize various recombinantly produced silk proteins, and many of these have also investigated their products for biomedical applications. This review gives a critical overview over of the results for applications of recombinant silk proteins in biomedical engineering.

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Spider silk and blend biomaterials: recent advances and future opportunities

Trossmann,

Scheibel

2024

Silk-Based Biomaterials for Tissue Engineering, Regenerative and Precision Medicine

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Water-based preparation of spider silk films as drug delivery matrices

Cited by 27 publications

References 41 publications

Advances in Plant-Derived Scaffold Proteins

Advances in Plant-Derived Scaffold Proteins

Biomedical Applications of Recombinant Silk‐Based Materials

Spider silk and blend biomaterials: recent advances and future opportunities

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