Advances in Plant-Derived Scaffold Proteins

Peng, Congyue Annie; Kozubowski, Lukasz; Marcotte, William R.

doi:10.3389/fpls.2020.00122

Cited by 6 publications

(3 citation statements)

References 186 publications

(203 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This material can be easily manipulated, and endogenous molecules can be encapsulated. Production of silk fibres is mainly made by extraction from lepidopteron insects of Bombycidae or Saturniidae families [ 218 ], but recombinant proteins are emerging [ 221 ].…”

Section: Three-dimensional Cell Culture Methodsmentioning

confidence: 99%

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

et al. 2020

View full text Add to dashboard Cite

Animal testing has long been used in science to study complex biological phenomena that cannot be investigated using two-dimensional cell cultures in plastic dishes. With time, it appeared that more differences could exist between animal models and even more when translated to human patients. Innovative models became essential to develop more accurate knowledge. Tissue engineering provides some of those models, but it mostly relies on the use of prefabricated scaffolds on which cells are seeded. The self-assembly protocol has recently produced organ-specific human-derived three-dimensional models without the need for exogenous material. This strategy will help to achieve the 3R principles.

show abstract

Section: Three-dimensional Cell Culture Methodsmentioning

confidence: 99%

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Additionally, the Zeta potential test showed a surface charge of 47mV, indicating the stability of the nanocarrier. These findings suggest that the chitosan-agarose nanocomposite hydrogel containing nanoclay has the potential for breast cancer treatment [14].…”

Section: Investigation Of Particle Size and Surface Charge In New Nan...mentioning

confidence: 75%

Assessing Particle Size and Surface Charge in Drug Carrier Nanoparticles for Enhanced Cancer Treatment: A Comprehensive Review Utilizing DLS and Zeta Potential Characterization

Majid Abdouss

2024

PSPRJ

View full text Add to dashboard Cite

The provided information underscores the significance of cancer control, including early detection, treatment, and prevention. The World Health Organization (W.H.O) is actively working towards improving access to screening, prevention, and treatment services, as well as raising awareness about the importance of cancer prevention and early detection. Researchers are also exploring the potential of natural substances to develop safer drugs for cancer treatment, with the W.H.O providing support to organizations involved in this research. One approach being explored is the use of Drug Delivery Systems (DDSs) to minimize damage to healthy tissue during cancer treatment. Maintaining a Zeta potential range between -30 and +30mV is considered optimal for nanocarriers in the bloodstream, as it ensures stability and long-term activity. Additionally, nanoparticles with a size below 200nm can be effectively distributed in the body and have an increased penetration rate into cancer cells. Smaller particles exhibit better mobility and can more efficiently reach their targets. A recent study analyzed the particle size and surface charge of newly designed drug nanocarriers to improve cancer treatment. This promising approach aims to optimize drug delivery and enhance the efficacy of cancer therapy.

show abstract

“…There is still no information about the recognition motifs and glycosylation patterns on spider silk proteins. Due to the lack of knowledge on spidroin PTMs, the PTM system of a production host might not fit the needs of the spider protein molecules ( Peng et al, 2020 ). Future studies of spider silk proteomes can provide more details concerning PTMs of spidroins contributing towards a better understanding of their effects on mechanical properties, fibre assembly and solubility as well as selection of appropriate expression hosts.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Spider Silk: Promises and Bottlenecks

Ramezaniaghdam

Nahdi

Reski

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Spider silk threads have exceptional mechanical properties such as toughness, elasticity and low density, which reach maximum values compared to other fibre materials. They are superior even compared to Kevlar and steel. These extraordinary properties stem from long length and specific protein structures. Spider silk proteins can consist of more than 20,000 amino acids. Polypeptide stretches account for more than 90% of the whole protein, and these domains can be repeated more than a hundred times. Each repeat unit has a specific function resulting in the final properties of the silk. These properties make them attractive for innovative material development for medical or technical products as well as cosmetics. However, with livestock breeding of spiders it is not possible to reach high volumes of silk due to the cannibalistic behaviour of these animals. In order to obtain spider silk proteins (spidroins) on a large scale, recombinant production is attempted in various expression systems such as plants, bacteria, yeasts, insects, silkworms, mammalian cells and animals. For viable large-scale production, cost-effective and efficient production systems are needed. This review describes the different types of spider silk, their proteins and structures and discusses the production of these difficult-to-express proteins in different host organisms with an emphasis on plant systems.

show abstract

Advances in Plant-Derived Scaffold Proteins

Cited by 6 publications

References 186 publications

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

Assessing Particle Size and Surface Charge in Drug Carrier Nanoparticles for Enhanced Cancer Treatment: A Comprehensive Review Utilizing DLS and Zeta Potential Characterization

Recombinant Spider Silk: Promises and Bottlenecks

Contact Info

Product

Resources

About