Water and sediment act as reservoirs for microbial taxa associated with invasive dreissenid mussels

Mathai, Prince P.; Magnone, Paolo; Dunn, Hannah M.; Sadowsky, Michael J.

doi:10.1016/j.scitotenv.2019.134915

Cited by 9 publications

(6 citation statements)

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“…The detected high diversity of mussel-associated assemblages (with many taxa not observed in water samples) is consistent with previous findings of specific and diverse bacterial communities associated with bivalves (Lokmer et al, 2016;Cleary and Polonia, 2018;Vezzulli et al, 2018;Mathai et al, 2020). Tenericutes, and more specifically Mycoplasma, abundant in the zebra mussel samples, are typical constituents of the core bivalve gut microbiome (Pierce, 2016;Aceves et al, 2018;Ward, 2018, 2019), including zebra mussels (Mathai et al, 2020). These obligate cell-associated bacteria are commonly found within a number of eukaryotic hosts and, although previously considered as parasites or even a sign of infection, are now assumed to be involved in mutually beneficial interactions with the host (Fraune and Zimmer, 2008;Holm and Heidelberg, 2016;van de Water et al, 2018).…”

Section: N 2 Fixation By Zebra Mussel Holobiontssupporting

confidence: 91%

“…The analysis of the microbiome associated with zebra mussels showed that comparatively few ASVs were shared with the waterborne microbial community. The detected high diversity of mussel-associated assemblages (with many taxa not observed in water samples) is consistent with previous findings of specific and diverse bacterial communities associated with bivalves (Lokmer et al, 2016;Cleary and Polonia, 2018;Vezzulli et al, 2018;Mathai et al, 2020). Tenericutes, and more specifically Mycoplasma, abundant in the zebra mussel samples, are typical constituents of the core bivalve gut microbiome (Pierce, 2016;Aceves et al, 2018;Ward, 2018, 2019), including zebra mussels (Mathai et al, 2020).…”

Section: N 2 Fixation By Zebra Mussel Holobiontssupporting

confidence: 90%

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Zebra Mussel Holobionts Fix and Recycle Nitrogen in Lagoon Sediments

et al. 2021

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Bivalves are ubiquitous filter-feeders able to alter ecosystems functions. Their impact on nitrogen (N) cycling is commonly related to their filter-feeding activity, biodeposition, and excretion. A so far understudied impact is linked to the metabolism of the associated microbiome that together with the host constitute the mussel’s holobiont. Here we investigated how colonies of the invasive zebra mussel (Dreissena polymorpha) alter benthic N cycling in the shallow water sediment of the largest European lagoon (the Curonian Lagoon). A set of incubations was conducted to quantify the holobiont’s impact and to quantitatively compare it with the indirect influence of the mussel on sedimentary N transformations. Zebra mussels primarily enhanced the recycling of N to the water column by releasing mineralized algal biomass in the form of ammonium and by stimulating dissimilatory nitrate reduction to ammonium (DNRA). Notably, however, not only denitrification and DNRA, but also dinitrogen (N2) fixation was measured in association with the holobiont. The diazotrophic community of the holobiont diverged substantially from that of the water column, suggesting a unique niche for N2 fixation associated with the mussels. At the densities reported in the lagoon, mussel-associated N2 fixation may account for a substantial (and so far, overlooked) source of bioavailable N. Our findings contribute to improve our understanding on the ecosystem-level impact of zebra mussel, and potentially, of its ability to adapt to and colonize oligotrophic environments.

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Section: N 2 Fixation By Zebra Mussel Holobiontssupporting

confidence: 91%

Section: N 2 Fixation By Zebra Mussel Holobiontssupporting

confidence: 90%

Zebra Mussel Holobionts Fix and Recycle Nitrogen in Lagoon Sediments

et al. 2021

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“…Both absolute temperatures and abrupt temperature changes can significantly impact rates of metabolism, immunological function, and disease susceptibility. Like other bivalves, ZMs harbor a large number of microbiota [21], some of which are postulated to play major roles in their growth and survival. It seems likely that changes in environmental factors (e.g., temperature) would also impact the composition of microbial communities associated with such organisms, either directly or indirectly.…”

Section: Introductionmentioning

confidence: 99%

Influence of Environmental Stressors on the Microbiota of Zebra Mussels (Dreissena polymorpha)

et al. 2020

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Host-associated microbiota play a critical role in host fitness by providing nutrition, enhancing digestion capabilities, and by providing protection from pathogens. Here, we investigated the effects of two environmental stressors, temperature, and salinity, on the microbiota associated with zebra mussels (ZMs), a highly invasive bivalve in North America. To examine this in detail, lake-collected ZMs were acclimated to laboratory conditions, and subjected to temperature and salinity stress conditions. The impact of these stressors on the diversity, composition, and dynamics of ZM-associated microbiota were assessed by using amplicon- and shotgun-based sequencing, and qPCR-based approaches. Elevated temperature was found to be the primary driver of ZM mortality, although salinity alone also increased its likelihood. Stressor-induced ZM mortality, which ranged between 53 and 100%, was concomitant with significant increases in the relative abundance of several genera of putative opportunistic pathogens including Aeromonas. These genera were only present in low relative abundance in ZMs obtained from the control tank with 0% mortality. Shotgun sequencing and qPCR analyses indicated that the relative and absolute abundances of pathogenic Aeromonas species (particularly A. veronii) were significantly greater in temperature-induced dead ZMs. Taken together, our results show that environmental stress, especially elevated temperature (> 25 °C), is associated with the rapid mortality of ZMs as well as the proliferation of putative opportunistic bacterial pathogens.

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“…Bray-Curtis dissimilarity matrices were used for principal coordinate analysis (PCoA). These matrices were also used to assess differences in beta diversity by analysis of similarity (ANOSIM) (Mathai et al, 2020). Canonical correspondence analysis (CCA) was done to visualize the relationships between life stages and taxa.…”

Section: Bioinformatics and Statistical Analysesmentioning

confidence: 99%

Gut Microbiota Associated With Different Sea Lamprey (Petromyzon marinus) Life Stages

et al. 2021

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Sea lamprey (SL; Petromyzon marinus), one of the oldest living vertebrates, have a complex metamorphic life cycle. Following hatching, SL transition into a microphagous, sediment burrowing larval stage, and after 2–10+ years, the larvae undergo a dramatic metamorphosis, transforming into parasitic juveniles that feed on blood and bodily fluids of fishes; adult lamprey cease feeding, spawn, and die. Since gut microbiota are critical for the overall health of all animals, we examined the microbiota associated with SLs in each life history stage. We show that there were significant differences in the gut bacterial communities associated with the larval, parasitic juvenile, and adult life stages. The transition from larval to the parasitic juvenile stage was marked with a significant shift in bacterial community structure and reduction in alpha diversity. The most abundant SL-associated phyla were Proteobacteria, Fusobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria, and Firmicutes, with their relative abundances varying among the stages. Moreover, while larval SL were enriched with unclassified Fusobacteriaceae, unclassified Verrucomicrobiales and Cetobacterium, members of the genera with fastidious nutritional requirements, such as Streptococcus, Haemophilus, Cutibacterium, Veillonella, and Massilia, were three to four orders of magnitude greater in juveniles than in larvae. In contrast, adult SLs were enriched with Aeromonas, Iodobacter, Shewanella, and Flavobacterium. Collectively, our findings show that bacterial communities in the SL gut are dramatically different among its life stages. Understanding how these communities change over time within and among SL life stages may shed more light on the role that these gut microbes play in host growth and fitness.

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Water and sediment act as reservoirs for microbial taxa associated with invasive dreissenid mussels

Cited by 9 publications

References 50 publications

Zebra Mussel Holobionts Fix and Recycle Nitrogen in Lagoon Sediments

Zebra Mussel Holobionts Fix and Recycle Nitrogen in Lagoon Sediments

Influence of Environmental Stressors on the Microbiota of Zebra Mussels (Dreissena polymorpha)

Gut Microbiota Associated With Different Sea Lamprey (Petromyzon marinus) Life Stages

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