“…Some possible explanations may be that the mixing of the soil profile during tillage may have reduced soil stratification and overridden the expected effect of fertilizer input by removing surficial soil NO 3 available for run‐off transport (e.g., Cade‐Menun, Carter, James, & Liu, ; Tiessen et al, ; Abdi, Cade‐Menun, Ziadi, & Parent, ). This may also reflect the dynamic nature of NO 3 , which is highly soluble so run‐off losses may not be not replenished (e.g., Li, Sivapalan, Tian, & Liu, 2010nov, ) and may be subject to leaching below the interaction zone (e.g., Ryan, Kachanoski, & Gillham, ; Kladivko et al, ), rapid uptake by plants, denitrification in anaerobic soils (Dawson & Murphy, ; Groffman & Tiedje, ), and nutrient management practices that minimize excess N inputs. Thus, consideration of the timing and type of nutrient input (mineral or organic) may be essential to predict its impact on run‐off concentrations.…”