Watching the Vessels Grow: Establishment of Intravital Microscopy in the Arteriovenous Loop Rat Model

Hessenauer, Maximilian; Vaghela, Ravikumar; Körner, Carolin; Hörsten, Stephan von; Pobel, Christoph R.; Gage, Daniel; Müller, Cláudia; Salehi, Sahar; Horch, Raymund E.; Arkudas, Andreas

doi:10.1089/ten.tec.2021.0024

Cited by 4 publications

(12 citation statements)

References 25 publications

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“…A comprehensive picture of ongoing cellular and molecular events can be created using appropriate tagging and microscopy, which can help to improve the tissue engineering process. Previously, we have developed an AV loop imaging chamber that allows the evaluation of ongoing vascular and cellular events on a real‐time basis 13,14 . Now in this study, for the first time, we were able to observe in vivo the microvascular development of neovessels that originated from the AV loop over a period of 21 days.…”

Section: Introductionmentioning

confidence: 81%

“…In the present study, we managed to even extend the imaging period of our previously developed AV loop IVM model 13 . We slightly modified the chamber, which can be installed subcutaneously on the left thigh and can be externalized for imaging.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we have developed an AV loop imaging chamber that allows the evaluation of ongoing vascular and cellular events on a real-time basis. 13,14 Now in this study, for the first time, we were able to observe in vivo the microvascular development of neovessels that originated from the AV loop over a period of 21 days. We were able to closely determine the onset of vascular development as well as the characteristics of initial network formation in the AV loop implanted into GelMA biomaterial.…”

mentioning

confidence: 83%

“…For this study, we slightly adapted the design of our previously developed IVM chamber to allow AV loop patency for a longer experimental period 13 . The basic design contains a circular chamber with five pins for holding the AV loop in the chamber.…”

Section: Methodsmentioning

confidence: 99%

“…Microcirculatory analysis was performed as described previously 13,17 . Intravital images were taken from each region of interest (ROI).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Microvascular development in the rat arteriovenous loop model in vivo—A step by step intravital microscopy analysis

Vaghela

Arkudas

Gage

et al. 2022

J Biomedical Materials Res

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The arteriovenous (AV) loop model is a key technique to solve one of the major problems of tissue engineering-providing adequate vascular support for a tissue construct of significant size. However, the molecular and cellular mechanisms of vascularization and factors influencing the generation of new tissue in the AV loop are still poorly understood. We previously established a novel intravital microscopy approach to study these events. In this study, we implanted our observation chamber filled with two types of hydrogels such as fibrin and methacrylate gelatin (GelMA) and performed intravital microscopy (IVM) on days 7, 14, and 21. Initial microvessel formation was observed in GelMA on day 14, while the vessel network showed clear indicators of network rearrangement and maturation on day 21. No visible microvessels were observed in fibrin. The chambers were explanted on day 21.Histological examination revealed higher numbers of microvessels in GelMA compared to fibrin, while the AV loop was thrombosed in all fibrin constructs, possibly due to matrix degradation. GelMA proved to be an ideal matrix for IVM studies in the AV loop model due to its slow degradation and transparency. This IVM model can be employed as a novel tool for live and thus faster comprehension of crucial events in the tissue regeneration process, which can improve tissue engineering application.

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Section: Introductionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

“…Microcirculatory analysis was performed as described previously 13,17 . Intravital images were taken from each region of interest (ROI).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Microvascular development in the rat arteriovenous loop model in vivo—A step by step intravital microscopy analysis

Vaghela

Arkudas

Gage

et al. 2022

J Biomedical Materials Res

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Recent advancement in vascularized tissue-engineered bone based on materials design and modification

Liu,

Chen,

Han

et al. 2023

Materials Today Bio

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Methods for experimental monitoring of tissue vascularization

Guseynov

Ивашкевич

Bopkhoev

et al. 2023

Medicinskij alfavit

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Microcirculation plays a decisive role in the metabolism processes between blood and tissue in both physiological and pathological conditions of the body. The analysis of changes in microcirculation provides a unique perspective for studying processes at the micro- and macrolevels in clinical and experimental medicine, including diseases in the maxillofacial region. Despite the critical role of microcirculation in many oral diseases, direct imaging and quantification of tissue microcirculation is currently limited. Scientific interest in microcirculatory monitoring is growing with the advent of new physical diagnostic methods. To date, there are many methods for assessing microcirculation. The purpose of this literature review is to show the positive and negative aspects of using various methods for monitoring microcirculation. The scientific literature was searched in the PubMed and Elibrary databases. As a result, the main shortcomings of various monitoring methods were emphasized, as well as what prospects for study and modification are possible today.

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Watching the Vessels Grow: Establishment of Intravital Microscopy in the Arteriovenous Loop Rat Model

Cited by 4 publications

References 25 publications

Microvascular development in the rat arteriovenous loop model in vivo—A step by step intravital microscopy analysis

Microvascular development in the rat arteriovenous loop model in vivo—A step by step intravital microscopy analysis

Recent advancement in vascularized tissue-engineered bone based on materials design and modification

Methods for experimental monitoring of tissue vascularization

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