Waste cooking oil and crude glycerol as efficient renewable biomass for the production of platform organic chemicals through oleophilic yeast strain of Yarrowia lipolytica

From crude glycerol and volatile fatty acids to biodiesel and other bioproducts using Yarrowia lipolytica NCYC 2904 as a cell factory

“…44 It should be noted that the FTIR spectrum of vegetable oil also showed the presence of bands at 1459.6 cm −1 , 1744.1 cm −1 , 2853.2 cm −1 , and 2923.3 cm −1 . Similar spectral peaks for microbial lipids extracted from Y. lipolytica as a potential feedstock for biodiesel production were also reported in other studies performed by Mitrea et al , 45 Radha et al , 44 and Vasaki et al 7…”

“…44 It should be noted that the FTIR spectrum of vegetable oil also showed the presence of bands at 1459.6 cm −1 , 1744.1 cm −1 , 2853.2 cm −1 , and 2923.3 cm −1 . Similar spectral peaks for microbial lipids extracted from Y. lipolytica as a potential feedstock for biodiesel production were also reported in other studies performed by Mitrea et al, 45 Radha et al, 44 and Vasaki et al 7 The FTIR spectrum of methyl esters in Y. lipolytica lipids has shown peaks at 2855.3 cm −1 and 2927.3 cm −1 , corresponding to sp 2 C-H and sp 3 C-H stretching, respectively. At 1740 cm −1 , the spectral peak represents the C]O stretch in the ester group as observed in the FTIR spectrum of Y. lipolytica lipids.…”

Succinic acid – A run-through of the latest perspectives of production from renewable biomass

“…The biosynthesis of the targeted dicarboxylic acid, namely SA, can be generated through the cellular tricarboxylic acid cycle (TCA-cycle or Krebs cycle) [ 8 ]. There are several studies that analyse the best method of succinate production from facultative-anaerobic to anaerobic fermentation technologies, with the first best known microorganisms including Actinobacillus succinogenes , Anaerobiospirillum succiniciproducens , Mannheimia succiniciproducens , Basfia succiniciproducens Yarrowia lipolytica , Pichia kudriavzevii , Clostridium spp., S .…”

“…In this context, extensive research on the fermentative production of organic products such as succinic acid (SA) has been conducted since the US Department of Energy (DOE) published its reports on bio-based chemicals [ 3 , 4 ]. Conventionally, SA was obtained by catalytic hydrogenation of malic anhydride, a fossil-based chemical [ 5 , 6 ], but nowadays SA is one of the most produced organic acids through biotechnological routes with a wide prevalence as bulk material in various industries, such as bioplastics, cosmetics, pharmaceuticals, and food industries [ 7 , 8 ]. According to the recent review paper of Narisetty et al (2022), the petrochemical SA's production costs were evaluated at €2554/MT, which is more expensive than bio-based SA synthesis (€1045/MT) [ 9 ].…”

“…As in the pharmaceutical industry, SA is used as feedstock for several chemicals, including adipic acid, 2-pyrrolidinone, succinate salts, 1,4-butanediol, maleic anhydride, etc. [ 8 , 16 , 17 ]. Moreover, one of the most recent applications of SA is related to the production of biodegradable plastic, polybutylene succinate, having properties comparable to polypropylene [ 7 ].…”