From crude glycerol and volatile fatty acids to biodiesel and other bioproducts using <i>Yarrowia lipolytica</i> NCYC 2904 as a cell factory

Pereira, Ana Sofia; Lopes, Marlene; Belo, Isabel

doi:10.1039/d3se00682d

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…Y. lipolytica is characterized by an efficient mechanism of uptake of hydrophobic substrates and their efficient metabolism, which consequently leads to lipid storage [6][7][8]. Due to their high lipid accumulation potential, this species has become a model organism, accumulating more than 20% of biomass in the form of triacylglycerides and sterol esters, which may be a promising alternative to commonly used fuels [9,10].…”

Section: Introductionmentioning

confidence: 99%

Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol

Rywińska,

Tomaszewska-Hetman,

Lazar

et al. 2024

IJMS

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Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species Yarrowia lipolytica. The aim of this study was to evaluate transformants of Y. lipolytica for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among Y. lipolytica species.

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Section: Introductionmentioning

confidence: 99%

Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol

Rywińska,

Tomaszewska-Hetman,

Lazar

et al. 2024

IJMS

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Exploring novel porphyrin photocatalyst for the conversion of frying oil to biodiesel: A step for globular economy

Patil,

Mataghare,

Maske

et al. 2024

Energy Conversion and Management

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Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate

Dias,

Lopes,

Fernandes

et al. 2024

Renewable Energy

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From crude glycerol and volatile fatty acids to biodiesel and other bioproducts using Yarrowia lipolytica NCYC 2904 as a cell factory

Abstract: Biodiesel production using bio-oil derived from low-cost feedstocks could provide a sustainable alternative for the replacement of conventional fossil fuels. This work studied fed-batch and two-stage batch cultures for bio-oil...

Cited by 4 publications

References 47 publications

Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol

Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol

Exploring novel porphyrin photocatalyst for the conversion of frying oil to biodiesel: A step for globular economy

Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate

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