Wall‐protein antibodies as inhibitors of growth and of autolytic reactions of isolated cell wall

Huber, Donald J.; Nevins, Donald J.

doi:10.1111/j.1399-3054.1981.tb02746.x

Cited by 38 publications

(19 citation statements)

References 21 publications

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“…Plant walls contain numerous hydrolytic enzymes, which have been viewed as catalysts capable of weakening the wall to permit turgor-driven extension (reviewed by Fry, 1988Fry, ,1989. In support of this hypothesis, Huber and Nevins (1981) and lnoue and Nevins (1991) found that antibodies raised against wall proteins could inhibit both auxin-induced growth and wall autolysis of corn coleoptiles. In addition, isolated walls from many species extend irreversibly when placed under tension in acid conditions (Rayle et al, 1970;Hager et al, 1971;Cleland et al, 1987), in a manner consistent with an enzyme-mediated process (Cosgrove, 1989).…”

Section: Introductionmentioning

confidence: 68%

Two endogenous proteins that induce cell wall extension in plants.

1992

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Plant cell enlargement is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wallloosening" enzymes. By employing a reconstitution approach, we found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicot stems and the leaves of amaryllidaceous monocots, but was less effective on grass coleoptile walls. Endogenous and reconstituted wall extension activities showed similar sensitivities to pH, metal ions, thiol reducing agents, proteases, and boiling in methanol or water. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD associated with the activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall. These proteins appear to mediate "acid growth" responses of isolated walls and may catalyze plant cell wall extension by a nove1 biochemical mechanism.

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Section: Introductionmentioning

confidence: 68%

Two endogenous proteins that induce cell wall extension in plants.

1992

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“…The cause-effect relationship between auxin-induced changes in chemical structure of cell wall polysaccharides and elongation growth has been examined using lectins (4,5), antibodies raised against cell wall polysaccharides (6), and proteins (3,8,11,12,15,22). The results obtained in the present study further confirm the usefulness of antibodies specific for cell wall components for understanding the mechanism by which auxin regulates elongation growth of higher plants.…”

Section: Specificity Of Antibodiesmentioning

confidence: 99%

Comparison of the Outer and Inner Epidermis

Hoson

Nevins²

1992

Plant Physiol.

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“…Antibodies were raised in female New Zealand rabbits by administration ofisolated proteins as reported earlier (10,21). Purified exo-and endoglucanases were separately emulsified with Freund's incomplete adjuvant (Difco Labs.)…”

Section: Preparation Of Glucanase Antibodiesmentioning

confidence: 99%

“…The solutions (1-10 AL) containing denatured proteins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10) Ag) were applied to a SDS-PAGE slab gel (0.75…”

Section: Sds-page and Western Blots Of Cell Wall Proteinsmentioning

confidence: 99%

“…However, the development ofantibodies offers an opportunity to conduct experiments that would complement the interpretation of results of experiments where inhibitors act to mimic substrates. Huber and Nevins (10) had first examined the physiological activity of antibodies generated in response to the administration of the LiCl extract of cell wall proteins to rabbits. These antibodies suppressed glucan autolysis and cell elongation ofmaize coleoptiles.…”

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Inhibition of Auxin-Induced Cell Elongation of Maize Coleoptiles by Antibodies Specific for Cell Wall Glucanases

Inouhe¹,

Nevins²

1991

Plant Physiol.

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Polyclonal antibodies were raised in rabbits in response to the administration of purified exo-and endoglucanases extracted from cell walls of maize (Zea mays L. B37 x Mo17) coleoptiles. Since the antibodies formed specific conjugates when challenged with the glucanase antigens in immunoblot assays they were employed to evaluate the participation of glucanases in tissue growth. lndole-3-acetic acid induced cell elongation of abraded coleoptile segments was inhibited when the antibodies were supplied as a short term pretreatment (25-200 microgram/milliliter of serum protein). The extent of inhibition of IAA induced cell elongation was additive when endo-and exoglucanase antibodies were applied together. The results suggest that both enzymes have a role in mediating IAA-induced cell elongation. Pretreatment with exo-and endoglucanases antibodies also inhibited IAA induced degradation of noncellulosic jO-D-glucans and the increased level of cellulosic polymers in maize coleoptiles. Antibodies also inhibited the expression of the autohydrolytic degradation of glucans in isolated cell walls. The extent of inhibition was dependent on the antibody concentration applied. The results support the contention that enzymatic processes mediated by exo-and endoglucanases are responsible for cell wall autolytic reactions and that these reactions are linked to the mechanism for expressing auxin induced cell elongation in maize coleoptiles.applied for this purpose because of its capacity to inhibit specific enzymes (20). Nojirimycin also suppressed both auxin-induced glucan degradation in vivo and cell elongation. However, the development ofantibodies offers an opportunity to conduct experiments that would complement the interpretation of results of experiments where inhibitors act to mimic substrates. Huber and Nevins (10) had first examined the physiological activity of antibodies generated in response to the administration of the LiCl extract of cell wall proteins to rabbits. These antibodies suppressed glucan autolysis and cell elongation ofmaize coleoptiles. An extension ofthat approach using more refined protein fractions reveals that only three maize cell wall proteins generate antibodies with the capability of inhibiting growth and for one of these a capacity for enzymatic activity has yet to be identified. Antibodies raised in response to specific wall glucanases offers an attractive approach for resolving their participation in key steps ofauxin responses (8).We evaluated the role of cell wall glucanases in glucan metabolism and in IAA-induced cell elongation of maize coleoptiles using antibodies raised against purified exo-and endoglucanases. MATERIALS AND METHODS Plant MaterialNoncellulosic ,B-D-glucans of cereal coleoptile cell walls are metabolized during cell extension (16,17,(22)(23)(24)26). While the precise role of glucan metabolism is not clear the process is governed by auxin and the key metabolic steps may relate directly to cell wall changes required for cell elongation ( 18-20). Cell walls isolated ...

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Wall‐protein antibodies as inhibitors of growth and of autolytic reactions of isolated cell wall

Cited by 38 publications

References 21 publications

Two endogenous proteins that induce cell wall extension in plants.

Two endogenous proteins that induce cell wall extension in plants.

Comparison of the Outer and Inner Epidermis

Inhibition of Auxin-Induced Cell Elongation of Maize Coleoptiles by Antibodies Specific for Cell Wall Glucanases

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