1991
DOI: 10.1104/pp.96.2.426
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Inhibition of Auxin-Induced Cell Elongation of Maize Coleoptiles by Antibodies Specific for Cell Wall Glucanases

Abstract: Polyclonal antibodies were raised in rabbits in response to the administration of purified exo-and endoglucanases extracted from cell walls of maize (Zea mays L. B37 x Mo17) coleoptiles. Since the antibodies formed specific conjugates when challenged with the glucanase antigens in immunoblot assays they were employed to evaluate the participation of glucanases in tissue growth. lndole-3-acetic acid induced cell elongation of abraded coleoptile segments was inhibited when the antibodies were supplied as a short… Show more

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Cited by 97 publications
(38 citation statements)
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“…This idea has been further supported in recent years by studies in which selective reagents were used to interfere with wall glycanase activity. Treatment of corn coleoptile segments with polyclonal antibodies against cell wall glycanases interfered with auxininduced growth, wall autolysis, and changes in wall viscoelasticity (Inouhe and Nevins, 1991). In a similar vein, antibodies and lectins that recognize xyloglucans in azuku bean (Hoson and Masuda, 1991) or (1+3, 1+4)-P-~-glucans in maize coleoptiles interfered with auxin-induced growth in excised sections.…”
Section: Do Wall-looseninc Enzymes Catalyze Wall Relaxation?mentioning
confidence: 94%
“…This idea has been further supported in recent years by studies in which selective reagents were used to interfere with wall glycanase activity. Treatment of corn coleoptile segments with polyclonal antibodies against cell wall glycanases interfered with auxininduced growth, wall autolysis, and changes in wall viscoelasticity (Inouhe and Nevins, 1991). In a similar vein, antibodies and lectins that recognize xyloglucans in azuku bean (Hoson and Masuda, 1991) or (1+3, 1+4)-P-~-glucans in maize coleoptiles interfered with auxin-induced growth in excised sections.…”
Section: Do Wall-looseninc Enzymes Catalyze Wall Relaxation?mentioning
confidence: 94%
“…Although the data obtained so far are sometimes more confusing than enlightening, antibodies offer a novel approach to probe how the interruption of normal orientation can disturb wall expansion. For example, Nevin's group has prepared antibodies to putative exo-and endo-glucanases purified from the walls of growing seedlings, and these antibodies partially reverse auxin-induced growth (Inouhe and Nevins, 1991). Antibodies to the p-o-glucan itself can also inhibit growth (Hoson and Nevins, 1989).…”
Section: Future Goals and Directionsmentioning
confidence: 99%
“…The cause-effect relationship between auxin-induced changes in chemical structure of cell wall polysaccharides and elongation growth has been examined using lectins (4,5), antibodies raised against cell wall polysaccharides (6), and proteins (3,8,11,12,15,22). The results obtained in the present study further confirm the usefulness of antibodies specific for cell wall components for understanding the mechanism by which auxin regulates elongation growth of higher plants.…”
Section: Specificity Of Antibodiesmentioning
confidence: 99%
“…Of those wall components that constitute Poaceae cell walls, the (1--3),(1l-4)-f-D-glucans are the prominent polysaccharides undergoing auxin-induced changes. Glucan metabolism has been observed during auxininduced elongation of Avena (16,23), Hordeum (24), Zea (12), and Oryza (26) coleoptiles. Two enzymes, an endo-and an exoglucanase, are involved in the hydrolysis of the polysaccharide in maize (Zea mays L.) coleoptiles (2,9,10).…”
mentioning
confidence: 99%
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