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Eriksson, Håkan

doi:10.1023/a:1008866717990

Cited by 8 publications

(11 citation statements)

References 3 publications

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“…However, Z-Y demonstrated a greater overall adsorption for both m -cresol and phenol when compared to that of mordenite (Figure a,b). These results are consistent with prior reports and are likely due to the 3D pore structure of Z-Y as opposed to the 2D structure found in mordenite . Z-Y did not absorb insulin, as demonstrated from HPLC analysis following Z-Y passage (97.8% ± 0.6) (Figure c) while providing minimal removal of zinc (Figure S1).…”

Section: Resultssupporting

confidence: 92%

“…The two types of zeolites evaluated were Z-Y and Mordenite. While both are aluminosilicates, they have their own unique properties . Each type of aluminosilicate is capable of binding both phenol and m -cresol (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…Zeolite preparation was modified from the protocol outlined by Eriksson . Briefly, zeolite Y and mordenite (Tosoh Corporation, Tokyo, Japan) were heated at 250 °C for 30 min prior to dextran coating (MW 40 000; Alfa Aesar, Ward Hill, MA) to ensure pyrogen free compounds.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Thus, this study endeavored to identify a mechanism for PP removal to minimize inflammation while maintaining protein stability and activity. We developed an efficient method of excipient removal by filtration immediately before insulin infusion using zeolites, based on previous studies by Eriksson . Zeolites are a type of porous aluminosilicate used commercially for their adsorptive and catalytic capabilities.…”

mentioning

confidence: 99%

“…Zeolites are a type of porous aluminosilicate used commercially for their adsorptive and catalytic capabilities. Zeolites are utilized in numerous applications ranging from petrochemical processing to medical devices for concentrating oxygen. − The porous structure of zeolite makes it an ideal filter material for size-exclusion filtration by trapping small components in the pores and allowing larger components to pass. Specifically, this study investigated the filtration efficiency of two types of zeolite, mordenite and zeolite Y, with further validation to determine protein adsorption in filtration materials and spectroscopic analysis monitoring protein structure.…”

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confidence: 99%

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Phenolic Preservative Removal from Commercial Insulin Formulations Reduces Tissue Inflammation while Maintaining Euglycemia

Mulka

Lewis

et al. 2021

ACS Pharmacol. Transl. Sci.

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Background: Exogenous insulin therapy requires stabilization of the insulin molecule, which is achieved through the use of excipients (e.g., phenolic preservatives (PP)) that provide protein stability, sterility and prolong insulin shelf life. However, our laboratory recently reported that PP, (e.g., m-creosol and phenol) are also cytotoxic, inducing inflammation and fibrosis. Optimizing PP levels through filtration would balance the need for insulin preservation with PP-induced inflammation. Method: Zeolite Y (Z-Y), a size-exclusion-based resin, was employed to remove PP from commercial insulin formulations (Humalog) before infusion. Results: PP removal significantly decreased cell toxicity in vitro and inflammation in vivo. Infusion site histological analysis after a 3 day study demonstrated that leukocyte accumulation increased with nonfiltered preparations but decreased after filtration. Additional studies demonstrated that a Z-Y fabricated filter effectively removed excess PP such that the filtered insulin solution achieved equivalent glycemic control in diabetic mice when compared to nonfiltered insulin. Conclusion: This approach represents the proof of concept that using Z-Y for in-line PP removal assists in lowering inflammation at the site of insulin infusion and thus could lead to extending the functional lifespan of insulin infusion sets in vivo.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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