Receptor-mediated endocytosis of particles by peripheral dendritic cells

Andersson, Linda; Hellman, Peter; Eriksson, Håkan

doi:10.1016/j.humimm.2008.07.010

Cited by 18 publications

(17 citation statements)

References 33 publications

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“…According to earlier reports, the zeolite particles used had an average hydrodynamic diameter of 600 nm [20] and previous reports have shown that zeolite particles associated with cells after washing are almost completely localized intra-cellular with only a minor fraction adsorbed on the cell surface [15,20,21]. Consequently, particles firmly associated with cells after incubation and washing, are demonstrability phagocytosed by the cells in question.…”

Section: Discussionmentioning

confidence: 92%

“…Coating of zeolites were done as earlier described [15,20,21] and briefly volumes of 1Âg supernatants corresponding to 1 mg zeolite particles were centrifuged for 5 min at 13,000Âg and the collected zeolites were suspended and incubated in 0.5 ml of Tris-buffer containing Alexa-OA or DQ-OA on a rocking table for 1 h using a final concentrations of 0.2 mg/ml Alexa-OA or DQ-OA. The zeolites were collected and washed twice with 1 ml PBS.…”

Section: Coating Zeolitesmentioning

confidence: 99%

“…Incubation of cells and zeolites were done as earlier described [15,20,21] and briefly cells, 1 Â 10 6 cells/ml in R10 medium, were incubated with ligand-coated zeolites in a final concentration of 40 lg/10 6 cells for 4 h at 37°C. As a control, cells were incubated for 4 h at 37°C with a control containing un-coated zeolite suspended in R10 as described above.…”

Section: Incubation With Zeolite Particlesmentioning

confidence: 99%

“…In this report, we describe and compare the antigen capturing capacity of freshly isolated mDCs and in vitro generated MoDCs with respect to different endocytosing mechanisms using dealuminated zeolite particles [15,20,21], immune complex and soluble molecules. Freshly isolated mDCs and MoDCs were phenotypically similar, and were also alike in the expression of co-stimulatory markers, but differed in the capacity to endocytose particles, immune complexes and soluble molecules.…”

Section: Introductionmentioning

confidence: 99%

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Myeloid blood dendritic cells and monocyte-derived dendritic cells differ in their endocytosing capability

et al. 2012

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a b s t r a c tHuman dendritic cells (DCs) constitute a heterogeneous population of antigen-presenting cells characterized by a unique capacity to stimulate naïve T cells. The functions of DCs depend on the particular subset and in this study we compare two types of myeloid DCs: freshly isolated blood mDCs and in vitro generated monocyte-derived DCs (MoDCs), in their ability to accomplish endocytosis.In our hands, these two DC subtypes showed similarities in the expression of surface markers, but displayed clear differences in endocytic capacity. Freshly isolated blood mDCs showed a high propensity to capture and endocytose particles compared to in vitro generated MoDCs. The blood mDCs also showed a clear receptor-enhanced endocytosis when zeolite particles were co-adsorbed with IgG. On the other hand, the MoDCs differed remarkably compared to blood mDCs in the capture of ovalbumin and immune complexes. Interestingly, the MoDCs showed low endocytosis of IgG-coated particles but an efficient capture of immune complexes. The MoDCs also showed a high capacity to capture ovalbumin although with a relatively low degree of internalization. These data indicate distinct differences in the early process of endocytosis featured by mDCs and MoDCs, which is important to consider when choosing DC populations for future functional or clinical applications. Ó

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Section: Discussionmentioning

confidence: 92%

Section: Coating Zeolitesmentioning

confidence: 99%

Section: Incubation With Zeolite Particlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Myeloid blood dendritic cells and monocyte-derived dendritic cells differ in their endocytosing capability

et al. 2012

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“…Another question refers to the efficient uptake of the nanoparticles. The particles enter the cells by endocytosis, a process which is influenced by the size of the nanoparticles 21 , the cell type and the differentiation status 22 and the cellular capability to perform phago-and macropinocytosis 23 . The optimum concentration to obtain a satisfying fluorescence signal has to be tested for each individual application or cell line.…”

Section: Discussionmentioning

confidence: 99%

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Lahm

Doppler

Dreßen

et al. 2015

JoVE

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The reprogramming of somatic cells to induced pluripotent stem cells (iPS) has successfully been performed in different mammalian species including mouse, rat, human, pig and others. The verification of iPS clones mainly relies on the detection of the endogenous expression of different pluripotency genes. These genes mostly represent transcription factors which are located in the cell nucleus. Traditionally, the proof of their endogenous expression is supplied by immunohistochemical staining after fixation of the cells. This approach requires replicate cultures of each clone at this early stage to preserve validated clones for further experiments. The present protocol describes an approach with genespecific nanoparticles which allows the evaluation of intracellular gene expression directly in live cells by fluorescence. The nanoparticles consist of a central gold particle coupled to a capture strand carrying a sequence complementary to the target mRNA as well as a quenched reporter strand. These nanoparticles are actively endocytosed and the target mRNA displaces the reporter strand which then start to fluoresce. Therefore, specific target gene expression can be detected directly under the microscope. In addition, the emitted fluorescence allows the identification, isolation and enrichment of cells expressing a specific gene by flow cytometry. This method can be applied directly to live cells in culture without any manipulation of the target cells.

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Nanocarriers and Immune Cells

Moll

Mailänder

2019

Biological Responses to Nanoscale Particles

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Receptor-mediated endocytosis of particles by peripheral dendritic cells

Cited by 18 publications

References 33 publications

Myeloid blood dendritic cells and monocyte-derived dendritic cells differ in their endocytosing capability

Myeloid blood dendritic cells and monocyte-derived dendritic cells differ in their endocytosing capability

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Nanocarriers and Immune Cells

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