von Wright’s Therapy to Jørgensen’s Syndrome

Maranhão, Juliano Souza de Albuquerque

doi:10.1007/s10982-008-9035-9

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…This speed was chosen to allow the complete separation of the creaming phase and the serum phase, without the precipitation of non-adsorbed protein. The protein concentration in the serum phase was determined using the Lowry method (Lowry, Rosebrough, Farr, & Randall, 1951). The surface protein loading τ (mg/m 2 ) can be calculated by the following equation:…”

Section: Surface Protein Loading (τ)mentioning

confidence: 99%

Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion

2016

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Zein colloidal nanoparticles can adsorb at the oil-water interface to form Pickering emulsion. However, zein Pickering emulsion is usually not stable due to the poor wettability of zein colloidal nanoparticles. The objective of this study was to modify the surface of zein nanoparticles using sodium caseinate (NaCas) and assess the properties of zein/NaCas nanocomplexes and the resultant oil-in-water Pickering emulsions. One percent (w/w) of zein/NaCas colloidal nanocomplexes were formed, with the zein:NaCas ratios (w/w) ranging from 10:1 to 10:4 at pH=3 by an ultrasound treatment. The zeta-potential of the zein/NaCas nanocomplexes showed altered surface charges, indicating that NaCas adsorbed on the surface of the zein colloidal nanoparticles. Three-phase contact angle measurements suggested that the original zein colloidal nanoparticles were preferentially wetted in water. The incorporation of 0.1%-0.2% (w/w) NaCas significantly enhanced its wettability in the oil, and intermediate wettability was achieved at a zein:NaCas ratio of 10:3. Confocal laser scanning microscopy (CLSM) showed that the incorporation of NaCas improved the interfacial coverage of the Pickering emulsions. When the zein:NaCas ratio ranged from 10:1 to 10:3, the interface was composed of zein/NaCas nanocomplexes. At a zein:NaCas ratio of 10:4, NaCas can competitively adsorbed to the interface and formed a hybrid interfacial structure. The Zein/NaCas nanocomplexes stabilized the Pickering emulsions and exhibited greater centrifugal stability than plain zein emulsions at most pHs and ionic strengths. The underlying mechanisms of the improved emulsion stability are discussed in this paper. This study explored a novel approach to stabilizing Pickering emulsions via the surface modification method using a foodgrade protein.

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Section: Surface Protein Loading (τ)mentioning

confidence: 99%

Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion

2016

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“…Thermo-Scientific protein kit based on modified Lowry et al method (Lowry et al, 1951). The color 7 developed in the sample is measured at 750 nm using a Thermo Scientific Evolution 220 UV-Visible 8 spectrophotometer.…”

Section: Sample Analyses 18mentioning

confidence: 99%

Low-temperature thermal pre-treatment of municipal wastewater sludge: Process optimization and effects on solubilization and anaerobic degradation

et al. 2017

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, Ray, M.B., Low-temperature thermal pre-treatment of municipal wastewater sludge: Process optimization and effects on solubilization and anaerobic degradation, Water Research (2017Research ( ), doi: 10.1016Research ( /j.watres.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The present study examines the relationship between the degree of solubilization and biodegradability of 11 wastewater sludge as a result of low-temperature thermal pre-treatment. The main effect of thermal pre-12 treatment is the disintegration of cell membranes and thus solubilization of organic compounds. There is 13 an established correlation between chemical oxygen demand (COD) solubilization and temperature of 14 thermal pre-treatment, but results of thermal pre-treatment in terms of biodegradability are not well 15 understood. Aiming to determine the impact of low temperature treatments on biogas production, the 16 thermal pre-treatment process was first optimized based on an experimental design study on waste 17 activated sludge in batch mode. The optimum temperature, reaction time and pH of the process were 18 determined to be 80 o C, 5 hr and pH 10, respectively. All three factors had a strong individual effect (p < 19 0.001), with a significant interaction effect for temp.pH 2 (p = 0.002). Thermal pre-treatments, carried out 20 on seven different municipal wastewater sludges at the above optimum operating conditions, produced 21 increased COD solubilization of 18.3 ± 7.5 % and VSS reduction of 27.7± 12.3 % compared to the 22 untreated sludges. The solubilization of proteins was significantly higher than carbohydrates. Methane 23 produced in biochemical methane potential (BMP) tests, indicated initial higher rates (p = 0.0013) for the 24 thermally treated samples (k hyd up to 5 times higher), although the ultimate methane yields were not 25 significantly affected by the treatment. 26 Process Optimization and Effects on Solubilization and Anaerobic

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“…Trehalose and glucose solutions (25-100 μg/ml) were used as standards. Protein levels of the samples were measured according to (Lowry et al 1951).…”

Section: Measurement Of the Intracellular Trehalose Levelmentioning

confidence: 99%

Involvement of small heat shock proteins, trehalose, and lipids in the thermal stress management in Schizosaccharomyces pombe

Glatz

Pilbat

Németh

et al. 2016

Cell Stress and Chaperones

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Changes in the levels of three structurally and functionally different important thermoprotectant molecules, namely small heat shock proteins (sHsps), trehalose, and lipids, have been investigated upon heat shock in Schizosaccharomyces pombe. Both α-crystallin-type sHsps (Hsp15.8 and Hsp16) were induced after prolonged high-temperature treatment but with different kinetic profiles. The shsp null mutants display a weak, but significant, heat sensitivity indicating their importance in the thermal stress management. The heat induction of sHsps is different in wild type and in highly heat-sensitive trehalose-deficient (tps1Δ) cells; however, trehalose level did not show significant alteration in shsp mutants. The altered timing of trehalose accumulation and induction of sHsps suggest that the disaccharide might provide protection at the early stage of the heat stress while elevated amount of sHsps are required at the later phase. The cellular lipid compositions of two different temperature-adapted wild-type S. pombe cells are also altered according to the rule of homeoviscous adaptation, indicating their crucial role in adapting to the environmental temperature changes. Both Hsp15.8 and Hsp16 are able to bind to different lipids isolated from S. pombe, whose interaction might provide a powerful protection against heat-induced damages of the membranes. Our data suggest that all the three investigated thermoprotectant macromolecules play a pivotal role during the thermal stress management in the fission yeast.

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von Wright’s Therapy to Jørgensen’s Syndrome

Cited by 9 publications

References 13 publications

Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion

Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion

Low-temperature thermal pre-treatment of municipal wastewater sludge: Process optimization and effects on solubilization and anaerobic degradation

Involvement of small heat shock proteins, trehalose, and lipids in the thermal stress management in Schizosaccharomyces pombe

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