Von Willebrand Factor in Cultured Human Vascular Endothelial Cells from Adult and Umbilical Cord Arteries and Veins

Pb, van Wachem; Jh, Reinders; Mf, van Buul-Wortelboer; Groot, de; Wg, van Aken; Ja, van Mourik

doi:10.1055/s-0038-1661637

Cited by 36 publications

(20 citation statements)

References 12 publications

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“…Although there were reports on cellular behavior dependence on the adsorbed fibronectin rather than on the underlying substrata, for example, Lewandowska et al reported that different surfaces bind similar amounts of fibronectin and cell attachment to these surfaces is similar [31], others found that the amount of cell-binding activity was the same for hydrophilic tissue cultures on polystyrene (TCPs) or hydrophobic polystyrene (BPS) at higher densities of adsorbed fibronectin [32][33][34]. In our study, both XPS results and ELISA assay demonstrated that the highest fibronectin was adsorbed on Fn-PHBHHx while its cell affinity was not the highest among all test films.…”

Section: Discussionmentioning

confidence: 99%

Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Liang

et al. 2005

Biomaterials

127

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Section: Discussionmentioning

confidence: 99%

Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Liang

et al. 2005

Biomaterials

127

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“…28 For example, differences have been found for the synthesis of prostacyclin, 29 vWF expression, 30 fibrinolytic activity, 31 • 3Z synthesis of platelet activating factor, 33 and the sensitivity for hormones. 34 For this reason, we studied endothelial cells isolated from human large vessels, from umbilical vein, and from microcirculation and compared them with SMC and Fb.…”

Section: Discussionmentioning

confidence: 99%

Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts.

Zwaginga¹,

Boer²,

Ijsseldijk³

et al. 1990

Arteriosclerosis

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When the endothelial cell layer Is damaged, a thrombotic reaction starts on the cells' subendothelium and on the connective tissue deposited by smooth muscle cells in the deeper layers. When more severe vascular damage occurs, hemostasis will Involve the vessel adventitia In which fibroblasts are found. In this article, the influence of in vitro cultured endothelial cells, smooth muscle cells, and fibroblasts on the hemostatic balance was studied. To do so, perfusions were performed with low molecular weight heparln antlcoagulated blood over the extracellular matrix of the cells. This method allowed the study of tissue factor-dependent thrombln generation and its influence on formation of fibrin and platelet aggregates. The experiments described in this article show that endothelial cells isolated from different human organs Interfere differently In the hemostatic response. Endothelial cells Isolated from umbilical veins are nonthrombogenic; they do not synthesize tissue factor under unstlmulated conditions. On their extracellular matrix, only adherent platelets are found, but no aggregates and no fibrin. Endothelial cells Isolated from omentum and atrium contain tissue factor activity under unstimulated conditions. As a consequence, thrombln Is generated on their surfaces, and platelet aggregates and fibrin deposition are found on the extracellular matrices after perfusions with whole blood. The matrix of smooth muscle cells and fibroblasts behaved similarly. Increase In shear rate and perfuslon time resulted In an Increase in platelet aggregate formation. Polymerized fibrin deposition decreased when perfusions were performed at higher shear. Both platelet aggregation and fibrin deposition were tissue factor dependent and could be blocked more than 70% by an antibody against tissue factor. Based on these results, we conclude that endothelial cells isolated from umbilical veins form the best nonthrombogenic surface In vitro. Moreover, coagulation-dependent hemostasis should be Included when thrombogenicity of subendothelium Is discussed, especially when it concerns matrix derived from cells present In the deeper layer of the vessel wall.

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“…Human umbilical vein endothelial cells (HUVECs) were isolated from umbilical veins according to the method of Jaffe et al 32 and modified by Van Wachem et al 33 In short, the umbilical vein was rinsed with PBS and incubated for 20 min at 37°C in 0.05% trypsin-0.02% EDTA solution in PBS. CMS was added to inactivate trypsin, and after exchange of the medium by fresh medium, cells were plated in fibronectincoated TCPS flasks and grown until the third passage.…”

Section: Isolation Of Endothelial Cellsmentioning

confidence: 99%

Adherence and Proliferation of Endothelial Cells on Surface-Immobilized Albumin-Heparin Conjugate

et al. 1998

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Small-diameter vascular grafts rapidly fail after implantation, due to occlusion caused by thrombosis. This problem cannot be overcome using medication. A promising improvement of graft patency is the seeding of endothelial cells (EC) on the luminal surface of the vascular graft. Conjugates of albumin and heparin, which were developed to obtain nonthrombogenic coatings, could form an ideal coating for vascular grafts. Besides presenting anticoagulant function, heparin will bind proteins with cell adhesive properties, thus facilitating adherence of EC to the graft surface. EC were able to grow to confluency on CO(2) gas plasma-treated polystyrene (PS-CO(2)) coated with albumin-heparin conjugate. CO(2) gas plasma treatment resulted in the introduction of functional groups at the surface (e.g., hydroxyl, aldehyde, carboxylic acid, and epoxide groups). Addition of albumin-heparin conjugate to the functionalized surface in an aqueous solution with pH 8.2 yielded a stable monolayer of covalently bound conjugate. The number of cells adhering and proliferating on this surface was comparable to the number of cells on fibronectin-coated PS-CO(2). However, the structure and size of EC proliferating on surface-immobilized albumin-heparin was more irregular. Long-term adherence might be improved by adding fibronectin to the albumin-heparin surface, either as a mixture with albumin-heparin or in a separate incubation step.

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Von Willebrand Factor in Cultured Human Vascular Endothelial Cells from Adult and Umbilical Cord Arteries and Veins

Cited by 36 publications

References 12 publications

Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts.

Adherence and Proliferation of Endothelial Cells on Surface-Immobilized Albumin-Heparin Conjugate

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