Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Qu, Xiang-Hua; Wu, Qiong; Liang, Juan Boo; Qu, Xue; Wang, Shen-Guo; Chen, Guoqiang

doi:10.1016/j.biomaterials.2005.05.034

Cited by 126 publications

(76 citation statements)

References 40 publications

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“…In vitro tests have demonstrated that PHBHHx has good biocompatibilities for several cells, including fibroblasts [15], chondrocytes [16] and osteoblasts [17]. However, most of the previous studies did not compare cell numbers between PHBHHx and TCPs [17][18][19][20][21][22][23][24][25]. One study showed that cell growth on PHBHHx film was not as good as that on TCPs [26].…”

Section: Discussionmentioning

confidence: 93%

“…The longer HHx pendant chain monomer negatively affected crystallization so that PHBHHx had lower crystallinity than PHB [43]. Previous research has demonstrated that the arrangement of the crystalline domain and the amorphous domain could affect the evaporating process of chloroform solvent during film preparation and determine the different polymer sur- face properties, including surface roughness, free energy, chemical states and hydrophilicity, and could subsequently influence protein adsorption, cell attachment and cell number [13,19,26]. Most of the components in PHBVHHx polymer were in amorphous state and did not involve in crystal-lattice formation.…”

Section: Discussionmentioning

confidence: 98%

“…Water contact angle of the tested polymer films was measured at 25°C with a contact angle meter (SL600, Solon Information Technology Co. Ltd., Shanghai, China) [19]. A 10 ll quantity of distilled water was gently dropped onto the surface of the films and the measurement time was less than 10 s. At least three measurements on different film locations were averaged for data analysis.…”

Section: Water Contact Angle Determinationmentioning

confidence: 99%

See 2 more Smart Citations

Biocompatibility of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) with bone marrow mesenchymal stem cells

Wei

Zhao

et al. 2009

Acta Biomaterialia

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Water Contact Angle Determinationmentioning

confidence: 99%

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Biocompatibility of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) with bone marrow mesenchymal stem cells

Wei

Zhao

et al. 2009

Acta Biomaterialia

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“…[8] In order to trigger the cell-matrix adhesion on PHA surfaces several modification techniques have been recently applied including alkaline hydrolysis, lipase treatment and plasma treatment. [9][10][11][12] Some of these treatments were followed by protein immobilization onto the polymeric surface.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Biodegradable Polyester for Tissue Engineering Applications

Thiré

Meiga

Dick

et al. 2007

Macromolecular Symposia

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Summary: Chemical modification of polymer surface may potentially be used to create smart materials that can guide cellular adhesion, proliferation and maintenance of specific expression of molecules. The microbial polyester poly (3-hydroxybutyrate) (PHB) has been attracted attention as promising material for applications in tissue engineering. In this work, a wet-chemical method, base ethylenediamine aminolysis, was performed to improve the adhesion of chondrocytes isolated from human articular cartilage to PHB films. The effects of chemical treatment on PHB films was evaluated by following changes in morphology and surface chemical composition using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS), respectively. While the effect on cells morphology was studied by scanning electron microscopy (SEM). The treatment with ethylenediamine did not change significantly the morphology of the structures of PHB films surface. However, the roughness of the aminolyzed films was slightly higher. The introduction of nitrogen-containing groups was confirmed by XPS. In vitro experiments indicated that the surface modification did not have toxic effects in cells, since they could adhere and proliferate on modified PHB films. It was observed that long-time treatment improved ability of PHB films to support cell growth, which could be accounted to physicochemical and topological effects.

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“…However, recent studies have revealed that cell adhesion does not depend only on the amount of adsorbed proteins but also on the appropriate conformation of adsorbed proteins as scaffolds. 27,28) Arima and Iwata summarized that cells effectively adhere to polymer surfaces presenting moderate wettability with water contact angles of 40-70 . 29) In our present results, there were no significant differences in ODvalues between a-C:H and up to a-C:H:F 60 surfaces with a contact angle of approximately 70…”

Section: )mentioning

confidence: 99%

Human Umbilical Vein Endothelial Cell Interaction with Fluorine-Incorporated Amorphous Carbon Films

Yoshimoto

Hasebe

Nagashima

et al. 2012

Jpn. J. Appl. Phys.

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A major clinical concern in coronary intervention for cardiovascular disease is late stent thrombosis after the implantation of drug eluting stents (DES). DES widely used in clinical settings currently utilize polymer coatings, which can induce persistent arterial wall inflammation and delayed vascular healing, resulting in impaired endothelialization. We examined the viability of human umbilical vein endothelial cells (HUVECs) for fluorine-incorporated amorphous carbon (a-C:H:F) coatings, which are known to be anti-thrombogenic. a-C:H:F and a-C:H were synthesized on the tissue culture dishes using radio frequency plasma enhanced chemical vapor deposition by varying the ratio of hexafluoroethane and acetylene. HUVECs were seeded on coated dishes for 6 days. The results indicate that the a-C:H:F surface does not disturb HUVEC proliferation in 6 days of culture and is promising for stent materials that allows the preservation of endothelialization, even if the fluorine concentration of a-C:H surface affects the early adhesion of endothelial cells.

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Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx)

Cited by 126 publications

References 40 publications

Biocompatibility of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) with bone marrow mesenchymal stem cells

Biocompatibility of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) with bone marrow mesenchymal stem cells

Functionalization of Biodegradable Polyester for Tissue Engineering Applications

Human Umbilical Vein Endothelial Cell Interaction with Fluorine-Incorporated Amorphous Carbon Films

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