Volumetric gas meter for laboratory-scale anaerobic bioreactors

Martínez-Sibaja, Albino; Alvarado-Lassman, Alejandro; Astorga‐Zaragoza, C.M.; Medina, Manuel Adam; Posada-Gómez, Rubén; Rodríguez-Jarquín, José Pastor

doi:10.1016/j.measurement.2011.08.018

Cited by 13 publications

(5 citation statements)

References 8 publications

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“…The total biogas volume was measured using a water displacement system (Martínez-Sibaja et al, 2011). The biogas composition in the headspace of the reactors was analyzed using a gas chromatograph (Focus GC, by Thermo Fisher Scientific, United States) equipped with a thermal conductivity detector (TCD) and a 3-m stainless steel column packed with Hayesep Q (800/100 mesh).…”

Section: Biogas and Organic Acid Measurementsmentioning

confidence: 99%

Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process

et al. 2020

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Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant-feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L −1 of sulfamethoxazole (SMX). Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH 4 and H 2 production than in the control. SMX was also degraded, and at the end of the experiment (69 days), just 20% of its initial concentration was found. The relative abundance (ARG/16S) of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading.

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Section: Biogas and Organic Acid Measurementsmentioning

confidence: 99%

Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process

et al. 2020

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“…Biogas volumetric production was measured using a water displacement device [ 41 ] and H 2 , CH 4 and CO 2 content (%) in the biogas was analyzed using a gas chromatograph (Focus GC, by Thermo Scientific, Waltham, MA, USA) equipped with a thermal conductivity detector (TCD) and a 3 m stainless-steel column packed with Hayesep Q (800/100 mesh). Nitrogen gas was used as a carrier at a flow rate of 35 mL min −1 .…”

Section: Methodsmentioning

confidence: 99%

Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community

et al. 2022

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Some livestock farms rely on anaerobic digestion (AD) technology for manure disposal, thus obtaining energy (biogas) and fertilizer (digestate). Mixtures of antibiotics used for animal health often occur in organic waste and their possible synergistic/antagonistic effects on microorganisms involved in AD are still poorly studied. This work focuses on the effects of adding ciprofloxacin, alone (5 mg L−1) and in combination with sulfamethoxazole (2.5–5–10 mg L−1), on AD efficiency and microbial community structure. The experiment consisted of 90-day cattle manure batch tests and antibiotic removal percentages were assessed. Adding antibiotics always promoted CH4 and H2 production compared to untreated controls; however, CH4 production was lowered with the highest ciprofloxacin (CIP) concentrations. The overall results show antibiotic degradation caused by acidogenic Bacteria, and CH4 was mainly produced through the hydrogenotrophic-pathway by methanogenic Archaea. Shifts in microbial community abundance (DAPI counts) and composition (Illumina-MiSeq and FISH analyses) were observed.

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“…Incubation substrate (450 mg) was dispensed into a 100 mL serum bottle with an appropriate amount of MSS and/or DF, and then 45 mL artificial rumen inoculum was transferred into each bottle under anaerobic conditions. Inoculated serum bottles (T1-T9; n = 4) and non-substrate control (TB; n = 3) were sealed with butyl rubber plugs and aluminum caps, and were incubated anaerobically for 24 h at 39 • C. Gas was collected at 2, 4, 6, 9, 12, 20, and 24 h using the water displacement method [20], and 1 mL fermentation gas was simultaneously extracted from the anaerobic serum bottles. The methane content at each time point was measured immediately by gas chromatography.…”

Section: In Vitro Rumen Incubationmentioning

confidence: 99%

Methane Emissions Regulated by Microbial Community Response to the Addition of Monensin and Fumarate in Different Substrates

2021

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Ruminants contribute significantly to global methane (CH4) emissions. This study aimed to evaluate the in vitro effects of monensin sodium salt (MSS) and disodium fumarate (DF) on CH4 production, rumen fermentation, and microbial community, with different substrates. The addition of MSS and DF, alone and in combination, significantly reduced the concentration and production of CH4 (p < 0.05), and while with vinasse as substrate, the CH4 production was higher for forage. The highest propionate production and lowest acetate and propionic ratio (A:P) values were all observed in cultures added to the combination of 14 mmol/L DF and 80 mg/kg MSS in both substrates, suggesting that these additives improved the rumen fermentation efficiency. The diversity indexes of prokaryotic microbiota with forage as the substrate were significantly higher than vinasse, and there were different effects on diversity indexes with the addition of MSS and DF depending on the incubated substrate. Supplementation with MSS and DF increased the number of starch degradation and fumarate reducing bacteria, decreased the number of methanogens, but had no significant effect on the number of fibrolytic bacteria. pH, NH3-N, and rumen volatile fatty acids (VFA) were the main factors influencing prokaryotic community structure. In conclusion, basal substrates (forage and vinasse) and CH4 mitigation additives (MSS and DF) have interactions on the in vitro rumen fermentation and microbial composition.

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Volumetric gas meter for laboratory-scale anaerobic bioreactors

Cited by 13 publications

References 8 publications

Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process

Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process

Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community

Methane Emissions Regulated by Microbial Community Response to the Addition of Monensin and Fumarate in Different Substrates

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