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Abstract:The high costs involved in treating wastewater are problems that developing countries confront, mainly in rural areas. Therefore, Constructed Wetlands (CWs), which are composed of substrate, vegetation, and microorganisms, are an economically and ecologically viable option for wastewater treatment in these places. There is a wide variety of possibilities for substrates and ornamental plants that have not yet been evaluated to be implemented in future CW designs. The goal of this study was to evaluate the process of adaptation and removal of wastewater pollutants in CW microcosms using different terrestrial ornamental plants (Lavandula sp., Spathiphyllum wallisii, and Zantedeschia aethiopica). Those plants were sown in two types of substrate: red volcanic gravel (RVG) and polyethylene terephthalate (PET). CWs with vegetation reduced 5-day biochemical oxygen demand (BOD 5 ) by 68% with RVG substrate and 63% with PET substrate, nitrates 50% in RVG substrate and 35% in PET substrate, phosphates 38% in RVG substrate and 35% in PET substrate, and fecal coliforms 64% in RVG and 59% in PET substrate). In control microcosms without vegetation, reductions were significantly lower than those in the presence of plants, with reduction of BOD 5 by 61% in RVG substrate and 55% in PET substrate, nitrates 26% in RVG substrate and 22% in PET substrate, phosphates 27% in RVG substrate and 25% in PET substrate. Concerning fecal coliforms 62% were removed in RVG substrate and 59% in PET substrate. Regarding the production of flowers, Lavandula sp. did not manage to adapt and died 45 days after sowing and did not produce flowers. Spathiphyllum wallisii produced 12 flowers in RVG and nine flowers in PET, while Zantedeschia aethiopica produced 10 in RVG and 7 in PET. These results showed that the use of substrates made of RVG and PET is a viable alternative to be implemented in CWs. In addition, the reuse of PET is an option that decreases pollution by garbage. The plants Spathiphyllum wallisii and Zantedeschia aethiopica remarkably contribute in the removal of pollutants in wastewater. Additionally, the use of ornamental plants, with commercial interest such as those evaluated, enables an added value to the CW to be given, which can be used for flower production purposes on a larger scale and favor its acceptance within rural communities.

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There are different schemes based on observers to detect and isolate faults in dynamic processes. In the case of fault diagnosis in instruments (FDI) there are different diagnosis schemes based on the number of observers: the Simplified Observer Scheme (SOS) only requires one observer, uses all the inputs and only one output, detecting faults in one detector; the Dedicated Observer Scheme (DOS), which again uses all the inputs and just one output, but this time there is a bank of observers capable of locating multiple faults in sensors, and the Generalized Observer Scheme (GOS) which involves a reduced bank of observers, where each observer uses all the inputs and m-1 outputs, and allows the localization of unique faults. This work proposes a new scheme named Simplified Interval Observer SIOS-FDI, which does not requires the measurement of any input and just with just one output allows the detection of unique faults in sensors and because it does not require any input, it simplifies in an important way the diagnosis of faults in processes in which it is difficult to measure all the inputs, as in the case of biologic reactors.

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This article describes the use of the electrical conductivity for measuring bed expansion in a continuous anaerobic biofilm reactor in order to prevent the exit of support material from the reactor with the consequent loss of biomass. The substrate used for the tests is obtained from a two-stage anaerobic digestion (AD) process at the pilot scale that treats the liquid fraction of fruit and vegetable waste (FVW). Tests were performed with the raw substrate before anaerobic treatment (S1), the effluent from the hydrolysis reactor (S2), and the effluent from the methanogenic reactor (S3) to evaluate its effect on the electrical conductivity values and its interaction with colonized support material. The tests were carried out in a 32 L anaerobic inverse fluidized bed reactor (IFBR), which was inoculated with colonized support material and using two industrial electrodes at different column positions. The results with the previously digested samples (S2 and S3) were satisfactory to detect the presence of support material at the points where the electrodes were placed since the electrical conductivity values showed significant changes of up to 0.5 V, while with substrate S1 no significant voltage differences were appreciated. These results demonstrate that electrical conductivity can be used as an economic and simple mean for monitoring the support material expansion in order to avoid over expansion in the IFBR. It was also demonstrated that the conditions of the substrate in the methanogenic stage (pH and presence of volatile fatty acids) do not affect the operation of the electrical conductivity detection system.

One of the inconveniences in the startup of methanogenic inverse fluidized‐bed reactors (IFBRs) is the long period required for biofilm formation and stabilization of the system. Previous researchers have preferred to start up in batch mode to shorten stabilization times. Much less work has been done with continuous‐mode startup for the IFBR configuration of reactors. In this study, we prepared two IFBRs with similar characteristics to compare startup times for batch‐ and continuous‐operation modes. The reactors were inoculated with a small quantity of colonized particles and run for a period of 3 months, to establish the optimal startup strategy using synthetic media as a substrate (glucose as a source of carbon). After the startup stage, the continuous‐ and batch‐mode reactors removed more than 80% of the chemical oxygen demand (COD) in 51 and 60 days of operation, respectively; however, at the end of the experiments, the continuous‐mode reactor had more biomass attached to the support media than the batch‐mode reactor. Both reactors developed fully covered support media, but only the continuous‐mode reactor had methane yields close to the theoretical value that is typical of stable reactors.
Then, a combined startup strategy was proposed, with industrial wastewater as the substrate, using a sequence of batch cycles followed by continuous operation, which allows stable operation at an organic loading rate of 20 g COD/L·d in 15 days. Using a fraction of colonized support as an inoculum presents advantages, with respect to previously reported strategies.

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