Volume‐dependent K+ and Cl‐ fluxes in rat thymocytes.

Soler, A; Rota, Rossella; Hannaert, Patrick; Cragoe, Edward J.; Garay, Ricardo P.

doi:10.1113/jphysiol.1993.sp019682

Cited by 18 publications

(37 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2) (22 + 0-5 x 10-5 mol 1-l) similar to that inhibiting K+-Cl-co-transport in rat thymocytes (Soler et al 1993) and red blood cells ( RVD can be described by means of two parameters: Cmax, the maximal RVD capacity and ti, the time where RVD equals Cmax/2 (Fig. 3).…”

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

“…We have previously found that rat thymocyte membranes have a 1: 1 K+-Cl-cotransport system (Soler, Rota, Hannaert, Cragoe & Garay, 1993). A similar cotransport system was well characterized in red blood cells, where it catalyses regulatory volume decrease (RVD; Kregenow, 1981;Lauf, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Pharmacological properties of RVD Figure 2 shows the effect of ion transport inhibitors on thymocyte RVD (see structures of compounds in Soler et al 1993 and ion flux IC50 in Table 1). It can be seen that DIOA was an efficient RVD inhibitor, with an IC50 of 2-2 + 0 5 x 10-5 mol I( mean+ S.D., n = 4).…”

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

“…Ion transport inhibitors and ionophores DIOA and the other compounds used in the study have been described previously (see Soler et al 1993 To study the effect of ion transport inhibitors and ionophores on cell volume regulation in rat thymocytes, the compounds were added to the incubation media from freshly prepared, concentrated stock solutions in water or dimethyl sulphoxide (DMSO), provided that the final concentrations of these solvents had no effect per se on cell volume (final DMSO concentration in the flux media was always lower than 0-1 %). In particular, DIOA was tested from stock solutions containing 50-500 mmol 1-1 of compounds in DMSO.…”

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

“…Na+-loaded-K+-depleted cells were prepared by using a previously published method (Soler et al 1993). …”

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

See 4 more Smart Citations

Cell volume regulation in rat thymocytes.

Arrázola

Rota

Hannaert

et al. 1993

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

SUMMARY1. DIOA (dihydroindenyl-oxy-alkanoic acid), a potent inhibitor of the K+-Cl-cotransport system, fully blocked regulatory volume decrease (RVD) in swelled rat thymocytes, with an IC50 of 2-2 + 0 5 x 10-5 mol 1-1 (mean + S.D., n = 4). Conversely, RVD was resistant to quinine, quinidine, apamin, cetiedil, amiloride, bumetanide and DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate).2. DIOA-sensitive RVD followed mono-exponential kinetics, with th (half-lifetime) of 1-3 min and maximal capacity (Cmax) of about 55 % of the initial cell swelling.Cmax and the initial rate of RVD (V.) were both linear functions of the increase in cell volume.3. RVD was: (i) slightly increased by replacing external Cl-by NO3-, (ii) reversed by replacing external Na+ by K+ (in the presence of external Cl-) and (iii) inhibited by cell K+ depletion. All these phenomena were blocked by DIOA (86 jmol 1-1).4. Increased membrane potassium permeability by valinomycin was unable to accelerate RVD or RVD reversal.5. In the presence of DIOA, thymocytes responded like osmometers (the relative cell volume was a linear function of the reciprocal of the relative osmolality) in a large range of osmolalities.6. The results strongly suggest that RVD in rat thymocytes is mediated by the K+-Cl-co-transport system.

show abstract

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

“…Na+-loaded-K+-depleted cells were prepared by using a previously published method (Soler et al 1993). …”

Section: The Effect Of Anisotonicity and Ion Substitutionmentioning

confidence: 99%

See 3 more Smart Citations

Cell volume regulation in rat thymocytes.

Arrázola

Rota

Hannaert

et al. 1993

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

Dramatic Magnesium Efflux Induced by High Potassium in Rat Thymocytes

Féray

Guerrouache

Garay

2000

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

A Mathematical Model of the Proton Balance in the Outer Mantle Epithelium of Anodonta cygnea L.

2008

View full text Add to dashboard Cite

In the freshwater mollusc Anodonta cygnea and other unionids, the mantle plays an important role in the regulation of the movements of ions between the shell and the extrapaleal fluid. In this report, a mathematical model that attempts to describe the cell metabolic mechanisms underlying the operation of the outer mantle epithelium as a source of protons is presented. We encoded the information gathered by studying the epithelium in vitro, which includes the electrophysiology of the preparation, measurements of basic rates of transport of protons and base, the effect of metabolic and transport inhibitors on its electrical behavior and the dynamic measurements of pHi. The model was conceived so that the short-circuit current (Isc) and fluxes of Na+, K+ and Cl(-); intracellular volume; electrical potential; and ionic concentrations can be computed as a function of time. Furthermore, the analytical descriptions of all ionic fluxes involved are such that the effect of transport inhibitors can be simulated. In all the simulations performed, it was possible to reproduce the experimental results obtained with specific inhibitors of transport systems on the Isc and on pHi. In some cases, it was necessary to make alterations to one or more parameters of the reference condition. For each simulation carried out, the analysis of the results was consistent. The model is an analytical tool that can be used to show the internal coherence of the qualitative model previously proposed and to plan further experiments.

show abstract

Volume‐dependent K+ and Cl‐ fluxes in rat thymocytes.

Cited by 18 publications

References 23 publications

Cell volume regulation in rat thymocytes.

Cell volume regulation in rat thymocytes.

Dramatic Magnesium Efflux Induced by High Potassium in Rat Thymocytes

A Mathematical Model of the Proton Balance in the Outer Mantle Epithelium of Anodonta cygnea L.

Contact Info

Product

Resources

About