Cell volume regulation in rat thymocytes.

Arrázola, Arantxa; Rota, Rossella; Hannaert, Patrick; Soler, A; Garay, Ricardo P.

doi:10.1113/jphysiol.1993.sp019683

Cited by 23 publications

(32 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is also true for crypt epithelia, as we have found that inhibition of either Clconductances with DIDS or tamoxifen (Fig. 4 B and C (25,26). When the CFTR -/-crypts were exposed to a hypotonic solution containing 1 ,uM valinomycin the RVD was restored (Fig.…”

Section: Resultsmentioning

confidence: 55%

Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.

Valverde

O’Brien

Sepúlveda

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Cystic fibrosis is a disease characterized by abnormalities in the epithelia of the lungs, intestine, salivary and sweat glands, liver, and reproductive systems, often as a result of inadequate hydration of their secretions. The primary defect in cystic fibrosis is the altered activity of a cAMP-activated Cl- channel, the cystic fibrosis transmembrane conductance regulator (CFTR) channel. However, it is not clear how a defect in the CFTR Cl- channel function leads to the observed pathological changes. Although much is known about the structural properties and regulation of the CFTR, little is known of its relationship to cellular functions other than the cAMP-dependent Cl- secretion. Here we report that cell volume regulation after hypotonic challenge is also defective in intestinal crypt epithelial cells isolated from CFTR -/- mutant mice. Moreover, the impairment of the regulatory volume decrease in CFTR -/- crypts appears to be related to the inability of a K+ conductance to provide a pathway for the exit of this cation during the volume adjustments. This provides evidence that the lack of CFTR protein may have additional consequences for the cellular function other than the abnormal cAMP-mediated Cl- secretion.

show abstract

Section: Resultsmentioning

confidence: 55%

Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.

Valverde

O’Brien

Sepúlveda

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…However, previous studies showed that at higher concentrations, i.e., 100 M, this drug can also partially inhibit Cl Ϫ and K ϩ channels (1,3,4). More specifically, a study showed that 100 M DIOA was able to reduce a swelling-activated Cl Ϫ conductance in human osteoblasts (4).…”

Section: Discussionmentioning

confidence: 99%

Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells

Ernest¹,

Weaver²,

Duyn³

et al. 2005

American Journal of Physiology-Cell Physiology

106

View full text Add to dashboard Cite

show abstract

“…AINI is the initial, instantaneous Tc increase expected at to for a perfect osmometric behavior. Although, the RVD might have a more complicated time course, we used this curve fitting procedure to characterize the rate of the volume regulatory processes (Arrazola, Rota, Hannaert, Soler, and Garay, 1993). Tc crgL is cell height in isoosmotic conditions before the reduction of'trb.…”

Section: Protocols For Tc Measurementsmentioning

confidence: 99%

Regulatory volume decrease in cultured kidney cells (A6): role of amino acids.

Smet

Simaels

Declercq

et al. 1995

The Journal of General Physiology

View full text Add to dashboard Cite

Volume regulation was studied in A6 epithelia grown on permeable supports by measuring cell thickness (To) while simultaneously recording short circuit current (Ix) and tmalsepithelial conductance (Gt). Lowering the tonicity of the basolateral solution (Wb) from 250 or 215 to 140 mOsm/kg elicited a rapid rise in Tc followed by a regulation of the cell volume towards control. This decrease in Tc displays the characteristics of the regulatory volume decrease (RVD). Upon restoring the isoosmotic conditions, T~ decreased rapidly below its control value. A post RVD regulatory volume increase (RVI) as described for other cell types was not observed. The subsequent reduction of the basolateral osmolality increased Tc to the level recorded at the end of the first hypoosmotic pulse. Because cell content was not altered during the isoosmotic period the second hypoosmotic challenge was isotonic with the cell and did therefore not evoke an RVD. However, the cell did not lose its ability to volume regulate since an RVD could be elicited by further reduction of 1rb from 140 to 100 mOsm/kg. The possibility of an involvement of amino acids in the RVD was tested. The amount of amino acids in the cell as well as excreted in the bath was determined by amino acid analysis. Millimolar concentrations of threonine, serine, alanine, glutamate, glycine and aspartate were found in the cell extract. The cellular amino acid concentration was 28.8 -+ 0.4 mM. The amounts of glycine, aspartate and glutamate excreted from the cell during the hypotonic treatment were significantly larger than in control conditions. The excretion of these amino acids during hypotonicity decreased the cellular amino acid concentration by 8.4 -+ 0.2 mM. This quantity cannot completely account for the RVD during the first hypotonic challenge. The addition of glycine, aspartate and glutamate to the bathing solutions, although used at concentrations higher than intracellularly, did not reduce RVD. On the contrary, this maneuver increased the amplitude of the RVD following both hypoosmotic pulses. This result suggests a stimulatory role of the amino acids on the processes responsible for the RVD.

show abstract

Cell volume regulation in rat thymocytes.

Cited by 23 publications

References 15 publications

Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.

Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.

Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells

Regulatory volume decrease in cultured kidney cells (A6): role of amino acids.

Contact Info

Product

Resources

About