Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster

Greco, Phillip G.; Meisel, Robert L.; Heidenreich, Byron A.; Garris, Paul A.

doi:10.1016/j.jneumeth.2005.08.009

Cited by 12 publications

(15 citation statements)

References 53 publications

(77 reference statements)

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“…This pattern presumably corresponds to the stimulating electrode descending ventrally into, and eventually through, the VTA. These results are consistent with the effect, on striatal DA release, of lowering the stimulating electrode through the VTA-SNc region of the rat (Garris et al, 1993) and the medial forebrain bundle (MFB) in the rat (Garris et al, 1993) and Syrian hamster (Greco et al, 2006). As illustrated by the Insets in Figure 6, although [CA] max changed during lowering of the stimulating electrode, electrically evoked CA dynamics did not, suggesting that the same neurochemical species was elicited and the same neuron type(s) was activated at each depth.…”

Section: Resultssupporting

confidence: 86%

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Real-time monitoring of electrically evoked catecholamine signals in the songbird striatum using in vivo fast-scan cyclic voltammetry

Smith

Garris

Casto

2015

Journal of Chemical Neuroanatomy

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Fast-scan cyclic voltammetry is a powerful technique for monitoring rapid changes in extracellular neurotransmitter levels in the brain. In vivo fast-scan cyclic voltammetry has been used extensively in mammalian models to characterize dopamine signals in both anesthetized and awake preparations, but has yet to be applied to a non-mammalian vertebrate. The goal of this study was to establish in vivo fast-scan cyclic voltammetry in a songbird, the European starling, to facilitate real-time measurements of extracellular catecholamine levels in the avian striatum. In urethane-anesthetized starlings, changes in catecholamine levels were evoked by electrical stimulation of the ventral tegmental area and measured at carbon-fiber microelectrodes positioned in the medial and lateral striata. Catecholamines were elicited by different stimulations, including trains related to phasic dopamine signaling in the rat, and were analyzed to quantify presynaptic mechanisms governing exocytotic release and neuronal uptake. Evoked extracellular catecholamine dynamics, maximal amplitude of the evoked catecholamine signal, and parameters for catecholamine release and uptake did not differ between striatal regions and were similar to those determined for dopamine in the rat dorsomedial striatum under similar conditions. Chemical identification of measured catecholamine by its voltammogram was consistent with the presence of both dopamine and norepinephrine in striatal tissue content. However, the high ratio of dopamine to norepinephrine in tissue content and the greater sensitivity of the carbon-fiber microelectrode to dopamine compared to norepinephrine favored the measurement of dopamine. Thus, converging evidence suggests that dopamine was the predominate analyte of the electrically evoked catecholamine signal measured in the striatum by fast-scan cyclic voltammetry. Overall, comparisons between the characteristics of these evoked signals suggested a similar presynaptic regulation of dopamine in the starling and rat striatum. Fast-scan cyclic voltammetry thus has the potential to be an invaluable tool for investigating the neural underpinnings of behavior in birds.

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Section: Resultssupporting

confidence: 86%

“…Similar to the striatum of the rat (Garris et al, 1994) and hamster (Greco et al, 2006), there is marked heterogeneity with regard to CFM depth and [CA] max electrically evoked by the 24-pulse, 60-Hz train. We also found an overall tendency for [CA] max to decline as the CFM was moved ventrally (Fig.…”

Section: Resultsmentioning

confidence: 99%

Real-time monitoring of electrically evoked catecholamine signals in the songbird striatum using in vivo fast-scan cyclic voltammetry

Smith

Garris

Casto

2015

Journal of Chemical Neuroanatomy

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“…Peterson and coworkers also found decreased dopamine release in the striatum of hypothyroid rats (Peterson et al, 2006). To determine dopamine release during exposure to hypoxia, microdialysis or a more rapid volammetric method to determine dopamine release (Greco et al, 2006). …”

Section: Discussionmentioning

confidence: 99%

Hypothyroidism attenuates SCH 23390-mediated depression of breathing and decreases d1 receptor expression in carotid bodies, PVN and striatum of hamsters

Schlenker

Schultz

2011

Brain Research

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Hypothyroidism can lead to depressed breathing. We determined if propylthiouracil (PTU)–induced hypothyroidism in hamsters (HH) altered dopamine D1 receptor expression, D1 receptor-modulated ventilation, and ventilatory chemoreflex activation by hypoxia or hypercapnia. Hypothyroidism was induced by administering 0.04% PTU in drinking water for three months. Ventilation was evaluated following saline or 0.25 mg/kg SCH 23390, a D1 receptor antagonist, while awake hamsters breathed normoxic (21% O2 in N2), hypoxic (10% O2 in N2) and hypercapnic (5% CO2 in O2) air. Relative to euthyroid hamsters (EH), HH exhibited decreased D1 receptor protein levels in carotid bodies, striatum, and hypothalamic paraventricular nucleus, but not in the nucleus tractus solitarius. Relative to EH, HH exhibited lower ventilation during exposure to normoxia, hypoxia, or hypercapnia, but comparable ventilatory responsiveness to chemoreflex activation. SCH 23390 decreased ventilation of EH hamsters exposed to normoxia, hypoxia, and hypercapnia. In HH SCH 23390 increased ventilation during baseline normoxia and did not affect ventilation during exposure to hypoxia and hypercapnia, resulting in reduced ventilatory responsivess to chemoreflex activation by hypoxia and hypercapnia. Furthermore, in HH D1 receptor protein levels are decreased in several brain regions and within the carotid bodies. Moreover, D1 receptor-modulation of breathing at rest and during gas exposures were depressed in EH but not HH.

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“…Many authors, beginning in the late 1970’s with R. N. Adams (Adams, 1976; Wightman et al, 1978), have attempted to track these changes, primarily focusing on dopamine (DA), by electrochemical means (Budygin et al, 2000; Budygin et al, 2001; Greco et al, 2006; Robinson et al, 2003). Other authors have also attempted to understand the role of certain ions, such as potassium and chloride, within neurological disorders (Gorji et al, 2006; Obrenovitch and Zilkha, 1995).…”

Section: Introductionmentioning

confidence: 99%

Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis

Crick¹,

Osorio²,

Frei³

et al. 2014

European Journal of Pharmaceutical Sciences

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Objectives The goal of this study was to use a status epilepticus steady-state chemical model in rats using the convulsant, 3-mercaptopropionic acid (3-MPA), and to compare the changes in striatal neurotransmission on a slow (5 minute) and fast (60 second) timescale. In vivo microdialysis was combined with electrophysiological methods in order to provide a complete evaluation of the dynamics of the results obtained. Objective To compare the effects of a steady-state chemical model pof status epilepticus on striatal amino-acid and amine neurotransmitters contents, as measured via in vivo microdialysis combined with electrophysiological methods. Measurements were performed on samples collected every 60 seconds and every 5 minutes. “Fast” (60s) and “slow” (5 min.) sampling timescales were selected, to gain more insight into the dynamics of GABA synthesis inhibition and of its effects on other neurotransmitters and on cortical electrical activity. Methods 3-MPA was administered in the form of an intra-venous load(60 mg/kg) followed by a constant infusion (50 mg/kg/min) for min. Microdialysis samples were collected from the striatum at intervals of 5 minutes and 60 seconds and analyzed for biogenic amine and amino acid neurotransmitters. ECoG activity was monitored via screws placed over the cortex. Results In the 5 minute samples, glutamate (Glu) increased and γ-aminobutyric acid (GABA) decreased monotonically while changes in dopamine (DA) concentration were bimodal. In the sixty second samples, Glu changes were bimodal, a feature that was not apparent with the five minute samples. ECoG activity was indicative of status epilepticus. Conclusions This study describes the combination of in vivo microdialysis with electrophysiology to monitor the effect of 3-MPA on neurotransmission in the brain. This led to a better understanding of the chemical changes in the striatum due to the applied 3-MPA chemical model of status epilepticus.

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Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster

Cited by 12 publications

References 53 publications

Real-time monitoring of electrically evoked catecholamine signals in the songbird striatum using in vivo fast-scan cyclic voltammetry

Real-time monitoring of electrically evoked catecholamine signals in the songbird striatum using in vivo fast-scan cyclic voltammetry

Hypothyroidism attenuates SCH 23390-mediated depression of breathing and decreases d1 receptor expression in carotid bodies, PVN and striatum of hamsters

Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis

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