Voltammetric and RP-LC assay for the antidepressant drug mirtazapine: A validated method for the pharmaceutical dosage form

Abstract

In the present paper, rapid, sensitive, selective, accurate and precise analytical methodologies were developed for the determination of the antidepressant drug mirtazapine (MIR) using voltammetry and liquid chromatography. In cyclic voltammetry (CV), MIR showed one sharp oxidation peak and one additional wave in acidic media in the anodic direction; at pH 5.50, the mirtazapine peak was single and sharp. Under optimized conditions, the peak current showed a linear dependence with concentration in the range between 0.212 and 26.54 µg/mL for glassy carbon (GC) and 1.06 and 26.54 µg/mL for boron-doped diamond (BDD) electrodes using differential pulse (DP) and square wave (SW) voltammetric techniques. The possible oxidation mechanisms are also discussed. A simple and fully validated reverse phase-liquid chromatography (RP-LC) method to test for MIR in tablets was developed using an X-Select RP-18 column (250x4.60 mm ID x 5µm) at 25ºC with methanol:water (30:70, v/v, containing 15 mM o-phosphoric acid) as the mobile phase adjusted to pH 3.0. The RP-LC method allowed quantification over a MIR concentration range of 1.0-18.0 µg/mL. MIR was exposed to thermal, photolytic, or oxidative stress, as well as acid and base hydrolysis conditions, and the stressed samples were assayed by the proposed LC method. The proposed methods allow for a number of cost- and time-saving benefits.

A Copper Oxide Nanoparticle Modified Screen-printed Electrode for Determination of Mirtazapine

“…The calculated LOQ was found to be 13.4 ng/ mL. These values were compared with other reported research work [26], Table 1. The intraday and interday precisions of the proposed method were in the range 0.71-1.23% and 0.75-1.35%, respectively.…”

“…MZ was analyzed in biological samples by using high performance liquid chromatography [19][20][21], gas chromatography [22], capillary electrophoresis [23], HPTLC [24] and spectroflouriomtry [25]. Ozkan et al performed a voltammeric assay for MZ in pharmaceutical dosage form by using glassy (GC) carbon and boron dopped diamond (BDD) electrodes [26].…”

Application of Titanium Oxide Decorated Multi‐walled Carbon Nanotubes/Polyaniline as a Transducer Polymer for the Potentiometric Determination of Mirtazapine

“…[3] Several techniques for MTZ determination in various matrices have been reported, including spectroscopy, [4] HPLC, [5] GC, [6] HPTLC, [7] LC-MS, [8] capillary electrophoresis [9] and voltammetry. [10] Many of these methods are complex and require derivatization procedures, sophisticated instrumentation, and pretreatment steps, as well as being time and laborintensive. According to the literature review, no potentiometric sensor has been applied for the quantitation of MTZ in different matrices.…”