Volatile metabolic profiling for discrimination of potato tubers inoculated with dry and soft rot pathogens

Lui, Lei; Vikram, A.; Abu-Nada, Y.; Kushalappa, Ajjamada C.; Raghavan, G.S.V.; Al-Mughrabi, Khalil I.

doi:10.1007/bf02894914

Cited by 50 publications

(33 citation statements)

References 18 publications

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“…carotovorum ( P. c. subsp. carotovorum ) [27], [28]. Volatile products may also play a role in vectoring soft rot pathogens since insects appear to play important roles in both Pectobacterium and Dickeya life cycles [29], [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40], [41].…”

Section: Introductionmentioning

confidence: 99%

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Marquez-Villavicencio

Weber

Witherell

et al. 2011

PLoS ONE

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Pectobacterium species are necrotrophic bacterial pathogens that cause soft rot diseases in potatoes and several other crops worldwide. Gene expression data identified Pectobacterium carotovorum subsp. carotovorum budB, which encodes the α-acetolactate synthase enzyme in the 2,3-butanediol pathway, as more highly expressed in potato tubers than potato stems. This pathway is of interest because volatiles produced by the 2,3-butanediol pathway have been shown to act as plant growth promoting molecules, insect attractants, and, in other bacterial species, affect virulence and fitness. Disruption of the 2,3-butanediol pathway reduced virulence of P. c. subsp. carotovorum WPP14 on potato tubers and impaired alkalinization of growth medium and potato tubers under anaerobic conditions. Alkalinization of the milieu via this pathway may aid in plant cell maceration since Pectobacterium pectate lyases are most active at alkaline pH.

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Section: Introductionmentioning

confidence: 99%

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Marquez-Villavicencio

Weber

Witherell

et al. 2011

PLoS ONE

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“…e healthy potato tubers (Solanum tuberosum L.) were used in this experiment. Initially, tubers appearing healthy and uniform in size (100-120 g) were selected and washed to remove excess soil, surface sterilized in 0.5% sodium hypochlorite solution for 10 min and rinsed in 3 changes of sterile distilled water (Lui and Kushalappa, 2002;Lui et al, 2005) and then air dried. en the tubers wounded with a cork borer with a diameter of 5 mm to a depth of 5 mm (Choiseul et al, 2007;Peters et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

Pathogenicity of Some Fusarium Species Associated with Superficial Blemishes of Potato Tubers

Gashgari¹,

Gherbawy²

2013

Pol J Microbiol

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A b s t r a c tAs an organ for reserve and propagation, the tuber grows underground and is in contact with soil-borne microorganisms, making it potentially exposed to blemishes. erefore, the objective of this study was the possibility of using some modern methods of molecular diagnostics and detection of the presence of fungal contaminants in potato blemishes in Al-Qasim (Saudi Arabia). Polygonal lesions were the most observed blemish type in the collected samples. One hundred and sixty isolates were recovered from di erent types of blemishes obtained in this study. Fusarium, Penicillium, Ilyonectria, Alternaria and Rhizoctonia were the most common genera collected from different blemish types. Using ITS region sequencing, all collected fungi were identified at the species level. All Fusarium strains collected during this study were used to detect their pathogenicity against potato tubers. is is the first comprehensive report on the identification of major pathogenic fungi isolated from potato tuber blemishes in Saudi Arabia.

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“…Fungal and oomycete diseases on a variety of hosts, including potatoes, apples, carrots, onions and mangoes, to name a few, have been subjected to headspace GC‐MS to discriminate the relevant pathogens (De Lacy Costello et al. , 2001; Lui et al. , 2005a, b; Moalemiyan et al.…”

Section: Metabolomicsmentioning

confidence: 99%

Assessing the impact of transcriptomics, proteomics and metabolomics on fungal phytopathology

Tan

Ipcho

Trengove

et al. 2009

Molecular Plant Pathology

120

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SUMMARYPeer-reviewed literature is today littered with exciting new tools and techniques that are being used in all areas of biology and medicine. Transcriptomics, proteomics and, more recently, metabolomics are three of these techniques that have impacted on fungal plant pathology. Used individually, each of these techniques can generate a plethora of data that could occupy a laboratory for years. When used in combination, they have the potential to comprehensively dissect a system at the transcriptional and translational level. Transcriptomics, or quantitative gene expression profiling, is arguably the most familiar to researchers in the field of fungal plant pathology. Microarrays have been the primary technique for the last decade, but others are now emerging. Proteomics has also been exploited by the fungal phytopathogen community, but perhaps not to its potential. A lack of genome sequence information has frustrated proteomics researchers and has largely contributed to this technique not fulfilling its potential. The coming of the genome sequencing era has partially alleviated this problem. Metabolomics is the most recent of these techniques to emerge and is concerned with the non-targeted profiling of all metabolites in a given system. Metabolomics studies on fungal plant pathogens are only just beginning to appear, although its potential to dissect many facets of the pathogen and disease will see its popularity increase quickly. This review assesses the impact of transcriptomics, proteomics and metabolomics on fungal plant pathology over the last decade and discusses their futures. Each of the techniques is described briefly with further reading recommended. Key examples highlighting the application of these technologies to fungal plant pathogens are also reviewed.

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Volatile metabolic profiling for discrimination of potato tubers inoculated with dry and soft rot pathogens

Cited by 50 publications

References 18 publications

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Pathogenicity of Some Fusarium Species Associated with Superficial Blemishes of Potato Tubers

Assessing the impact of transcriptomics, proteomics and metabolomics on fungal phytopathology

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