The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Marquez-Villavicencio, Maria del Pilar; Weber, Brooke; Witherell, R. Andrews; Willis, David K.; Charkowski, Amy O.

doi:10.1371/journal.pone.0022974

Cited by 64 publications

(68 citation statements)

References 76 publications

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“…This was for instance observed for Pectobacterium species, bacterial pathogens responsible for soft rot disease in potato. Disruption of the biosynthesis of the volatile Volatile affairs in microbial interactions R Schmidt et al 2,3-butanediol coincided with reduced virulence (Marquez-Villavicencio et al, 2011). Volatile compounds can also have a role in the attraction of other microorganisms.…”

Section: Volatile Affairs In Microbial Interactions R Schmidt Et Almentioning

confidence: 99%

Volatile affairs in microbial interactions

et al. 2015

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Microorganisms are important factors in shaping our environment. One key characteristic that has been neglected for a long time is the ability of microorganisms to release chemically diverse volatile compounds. At present, it is clear that the blend of volatiles released by microorganisms can be very complex and often includes many unknown compounds for which the chemical structures remain to be elucidated. The biggest challenge now is to unravel the biological and ecological functions of these microbial volatiles. There is increasing evidence that microbial volatiles can act as infochemicals in interactions among microbes and between microbes and their eukaryotic hosts. Here, we review and discuss recent advances in understanding the natural roles of volatiles in microbe-microbe interactions. Specific emphasis will be given to the antimicrobial activities of microbial volatiles and their effects on bacterial quorum sensing, motility, gene expression and antibiotic resistance.

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Section: Volatile Affairs In Microbial Interactions R Schmidt Et Almentioning

confidence: 99%

Volatile affairs in microbial interactions

et al. 2015

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“…Pectobacterium and Dickeya, like many bacteria, encode the butanediol pathway, which results in the production of the potent insect attractant acetoin, suggesting that these bacteria may attract insect vectors to infected plant material through this route (37,100). Once associated with an insect, some isolates of Pectobacterium carotovorum can infect and persist in D. melanogaster and activate an immune response (8,9).…”

Section: Insects As Vectors and Alternate Hostsmentioning

confidence: 99%

“…Later, Liu et al (88) showed that AHL-mediated quorum sensing regulates one quarter of Pectobacterium genes, including many virulence genes. AHL is unstable at alkaline pH (15) and SRE raise the pH of their environment during infection (100,109,118), so the role AHL plays during disease may be transient.…”

Section: Intercellular Bacterial Signal Moleculesmentioning

confidence: 99%

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Charkowski¹,

Blanco

Condemine

et al. 2012

Annu. Rev. Phytopathol.

215

251

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Soft-rot Enterobacteriaceae (SRE), which belong to the genera Pectobacterium and Dickeya, consist mainly of broad host-range pathogens that cause wilt, rot, and blackleg diseases on a wide range of plants. They are found in plants, insects, soil, and water in agricultural regions worldwide. SRE encode all six known protein secretion systems present in gram-negative bacteria, and these systems are involved in attacking host plants and competing bacteria. They also produce and detect multiple types of small molecules to coordinate pathogenesis, modify the plant environment, attack competing microbes, and perhaps to attract insect vectors. This review integrates new information about the role protein secretion and detection and production of ions and small molecules play in soft-rot pathogenicity.

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“…In fact, the latter bacteria follow the mixed-acid fermentation route during the early stages of growth and switch to the 2,3-butanediol route in the late-exponential phase (3,4). This switch is generally viewed as a strategy of these bacteria to prevent excessive medium acidification, and it has been demonstrated that knockout of the 2,3-butanediol pathway indeed results in more-pronounced acidification, early growth arrest, and cell death (5,6).…”

mentioning

confidence: 99%

Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

Vivijs

Moons

Aertsen

et al. 2014

Appl Environ Microbiol

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Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively less acidic and more neutral end products, such as acetoin and 2,3-butanediol. In this work, we equipped Escherichia coli MG1655 with the budAB operon, encoding the acetoin pathway, from Serratia plymuthica RVH1 and investigated how this affected the ability of E. coli to cope with acid stress during growth. Acetoin fermentation prevented lethal medium acidification by E. coli in lysogeny broth (LB) supplemented with glucose. It also supported growth and higher stationary-phase cell densities in acidified LB broth with glucose (pH 4.10 to 4.50) and in tomato juice (pH 4.40 to 5.00) and reduced the minimal pH at which growth could be initiated. On the other hand, the acetoin-producing strain was outcompeted by the nonproducer in a mixed-culture experiment at low pH, suggesting a fitness cost associated with acetoin production. Finally, we showed that acetoin production profoundly changes the appearance of E. coli on several diagnostic culture media. Natural E. coli strains that have laterally acquired budAB genes may therefore have escaped detection thus far. This study demonstrates the potential importance of acetoin fermentation in the ecology of E. coli in the food chain and contributes to a better understanding of the microbiological stability and safety of acidic foods.

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The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Cited by 64 publications

References 76 publications

Volatile affairs in microbial interactions

Volatile affairs in microbial interactions

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

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