Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Alwis, Ransika De; Fujita, Kazuo; Ashitani, Tatsuya; Kuroda, Kenichi

doi:10.1016/j.jplph.2008.09.009

Cited by 26 publications

(15 citation statements)

References 30 publications

(30 reference statements)

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“…As reported for the improvement of hypericins and hyperforins production (Kirakosyan et al 2000;Gadzovska et al 2007), the biosynthesis of EO can also be enhanced through the addition of elicitors to the culture media (Rao and Ravishankar 1999;Thimmaraju et al 2005;Banchio et al 2009;De Alwis et al 2009) .…”

Section: Biotechnology In Hypericum Essential Oil Improvementmentioning

confidence: 88%

Hypericum sp.: essential oil composition and biological activities

2012

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Phytochemical composition of Hypericum genus has been investigated for many years. In the recent past, studies on the essential oils (EO) of this genus have been progressing and many of them have reported interesting biological activities. Variations in the EO composition of Hypericum species influenced by seasonal variation, geographic distribution, phenological cycle and type of the organ in which EO are produced and/or accumulated have also been reported. Although many reviews attributed to the characterization as well as biological activities of H. perforatum crude extracts have been published, no review has been published on the EO composition and biological activities of Hypericum species until recently (Crockett in Nat Prod Commun 5(9): 1493-1506, 2010 Bertoli et al. in Global Sci Books 5:29-47, 2011). In this article, we summarize and update information regarding the composition and biological activities of Hypericum species EO. Based on experimental work carried out in our laboratory we also mention possible biotechnology approaches envisaging EO improvement of some species of the genus.

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Section: Biotechnology In Hypericum Essential Oil Improvementmentioning

confidence: 88%

Hypericum sp.: essential oil composition and biological activities

2012

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“…The information available for α-thujene (Raffa and Berryman 1982a;Zhao et al 2010) suggests that conifers do not use it as a defensive compound, but it may have some antifungal activity (Bajpai et al 2007). Sabinene (De Alwis et al 2009;Espinosa-garcia and Langenheim 1991;Kohzaki et al 2009) and terpinolene (Davis and Hofstetter 2011;Viiri et al 2001) are among the most induced compounds in some conifers under fungal attack, and possess antifungal properties against several phytopathogens and fungal endophytes RT retention time. Numbers and letters in bold type indicate statistically significant differences (REML, fixed = treatment, random = genotype, paired Tukey's post-hoc test, P<0.05) RT retention time.…”

Section: Discussionmentioning

confidence: 99%

“…NA not available. Numbers and letters in bold type indicate statistically significant differences (REML, fixed = treatment, random = genotype, paired Tukey's post-hoc test, P<0.05) and marginally significant differences (P<0.10, in italics) (Bridges 1987;De Alwis et al 2009;Espinosa-garcia and Langenheim 1991;Kohzaki et al 2009;Paine and Hanlon 1994). Herbicide application also can increase the concentration of terpinen-4-ol in P. ponderosa (Kidd and Reid 1979), a compound with remarkable biological activity on fungi (Kusumoto et al 2014;Morcia et al 2012;Nenoff et al 1996) and bacteria (Kotan et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

Achotegui-Castells

Danti²,

Llusià

et al. 2015

J Chem Ecol

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Seiridium cardinale, the main fungal pathogen responsible for cypress bark canker, is the largest threat to cypresses worldwide. The terpene response of canker-resistant clones of Italian cypress, Cupressus sempervirens, to two differently aggressive isolates of S. cardinale was studied. Phloem terpene concentrations, foliar terpene concentrations, as well as foliar terpene emission rates were analyzed 1, 10, 30, and 90 days after artificial inoculation with fungal isolates. The phloem surrounding the inoculation point exhibited de novo production of four oxygenated monoterpenes and two unidentified terpenes. The concentrations of several constitutive mono-and diterpenes increased strongly (especially α-thujene, sabinene, terpinolene, terpinen-4-ol, oxygenated monoterpenes, manool, and two unidentified diterpenes) as the infection progressed. The proportion of minor terpenes in the infected cypresses increased markedly from the first day after inoculation (from 10 % in the control to 30-50 % in the infected treatments). Foliar concentrations showed no clear trend, but emission rates peaked at day 10 in infected trees, with higher δ-3-carene (15-fold) and total monoterpene (10-fold) emissions than the control. No substantial differences were found among cypresses infected by the two fungal isolates. These results suggest that cypresses activate several direct and indirect chemical defense mechanisms after infection by S. cardinale.

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“…For example, yeast extract improved saponin production in Panax ginseng cell suspension cultures without affecting cell growth (Lu et al 2001). Yeast extract also induced formation of aucuparin as the major phytoalexin in Sorbus aucuparia cell cultures (Huttner et al 2010), and stimulated the biosynthesis of monoterpenes in Cupressus lusitanica cell cultures (De Alwis et al 2009). Poly-or oligosaccharides are signaling molecules with elicitation pathways that have been intensively studied because these compounds can substitute for fungal elicitors during a pathogen attack (Zhao et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Cai¹,

Kastell²,

Mewis

et al. 2011

Plant Cell Tiss Organ Cult

112

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The effects of yeast extract and selected polysaccharide elicitors on secondary metabolite production, particularly of anthocyanin and phenolic acid, in cell suspension cultures of Vitis vinifera were investigated. All elicitors either maintained or promoted cell growth in culture. Overall, secondary metabolite production in V. vinifera cell suspension cultures responded differently to different elicitors. Chitosan, pectin, and alginate enhanced production of anthocyanin within 13 days of culture with levels of 2.5-, 2.5-, and 2.6-fold increase, respectively, over that of control. Chitosan, alginate, and gum arabic significantly promoted accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol, in V. vinifera cultures, as well as in the culture medium. Intracellular phenolic acid production was significantly enhanced by alginate and chitosan, with 1.7-and 1.5-fold levels, respectively, of that of control. Extracellular phenolic acid production was also significantly increased in the presence of chitosan and gum arabic, with levels of 3.3-and 1.7-fold higher, respectively, than those of control. In addition, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was enhanced in the presence of elicitors, and this was positively correlated with increased accumulation of anthocyanin in V. vinifera cell suspension cultures.

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Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Cited by 26 publications

References 30 publications

Hypericum sp.: essential oil composition and biological activities

Hypericum sp.: essential oil composition and biological activities

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

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