Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Abstract

The effects of yeast extract and selected polysaccharide elicitors on secondary metabolite production, particularly of anthocyanin and phenolic acid, in cell suspension cultures of Vitis vinifera were investigated. All elicitors either maintained or promoted cell growth in culture. Overall, secondary metabolite production in V. vinifera cell suspension cultures responded differently to different elicitors. Chitosan, pectin, and alginate enhanced production of anthocyanin within 13 days of culture with levels of 2.5-, 2.5-, and 2.6-fold increase, respectively, over that of control. Chitosan, alginate, and gum arabic significantly promoted accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol, in V. vinifera cultures, as well as in the culture medium. Intracellular phenolic acid production was significantly enhanced by alginate and chitosan, with 1.7-and 1.5-fold levels, respectively, of that of control. Extracellular phenolic acid production was also significantly increased in the presence of chitosan and gum arabic, with levels of 3.3-and 1.7-fold higher, respectively, than those of control. In addition, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was enhanced in the presence of elicitors, and this was positively correlated with increased accumulation of anthocyanin in V. vinifera cell suspension cultures.

Effects of Foliar of the Application Chitosan and Reduced Irrigation on Essential Oil Yield, Total Phenol Content and Antioxidant Activity of Extracts From Green and Purple Basil

“…1). Cai et al [2011] have been reported that treatment with chitosan efficiently enhanced biosynthesis of phenolic compounds in Vitis vinifera. Similarly, Kim et al [2003] reported that total amount of the phenolic and terpenic compounds in sweet basil increased after the chitosan treatment.…”

Methyl jasmonate elicits the solasodine production and anti-oxidant activity in hairy root cultures of Solanum trilobatum L.

“…The biomass increase was indirectly ascertained by a gradual decrease in the conductivity of the culture medium at various time points for all the cultured root clones. Decrease in conductivity of medium can be attributed to the consumption of inorganic and mineral nutrients in the culture medium (Cai et al, 2012). The initial conductivity of MS medium was 5.9 ± 0.09 ms, but upon increase in biomass due to uptake of nutrients, the conductivity of medium progressively decreased to 2.986 ± 0.12 ms after 6 weeks.…”

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

“…Chitin as b-1,4-linked polymer of N-acetylglucosamine and chitosan (deacetylated chitin) are biotic elicitors extensively studied for their effects on secondary metabolite production in various plant cell cultures (Korsangruang et al 2010;Zhao et al 2010;Lim et al 2013). Pectin as a complex structural polysaccharide composed of b-1,4-linked galacturonic acid subunits was found as effective elicitor in enhancement of plant bioactive compounds in cell suspensions (Wiktorowska et al 2010;Cai et al 2012;Veerashree et al 2012). Dextran describes preparations of D-glucans that contain substantial numbers of 1,6-linked a-D-glucopyranosyl residues.…”