Abstract:We have developed a cryopreservation method that uses a single-component super-adiabatic two-fluid de Laval nozzle for the continuous production of solid nitrogen particles with a micro-nano-order particle size. The cell viability results showed that use of the solid nitrogen spray improved cell viability at the time of thawing by more than 20% compared with the conventional liquid nitrogen immersion freezing method. By using the present dynamic spray cooling method that avails of the synergistic effect of imp… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.