Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn,<i>Macrobrachium rosenbergii</i>, and stimulation by 4-nonylphenol

Ara, Ferdause; Damrongphol, Praneet

doi:10.1111/j.1365-2109.2012.03229.x

Cited by 17 publications

(7 citation statements)

References 27 publications

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“…Furthermore, although lower than observed for the ovary, qPCR analysis reveals Vtg1 expression is clearly evident in the hepatopancreas, while the expression signal corresponding to Vtg2 cannot clearly be distinguished from the low-level amplification of non-target sequences. These findings are consistent with studies that show the ovary and hepatopancreas are major sites of Vtg synthesis in crustaceans (eg Fainzilber et al, 1992;Chen et al, 1999;Tsutsui et al, 2000;Tseng et al, 2001;Abdu et al, 2002;Tseng et al, 2002;Tsang et al, 2003;Serrano-Pinto et al, 2004;Tsutsui et al, 2004;Mak et al, 2005;Kang et al, 2008;Raviv et al, 2006;Phiriyangkul and Utarabhand, 2006;Tiu et al, 2006;Xie et al, 2009;Ara and Damrongphol, 2012) and suggest that the ovary is the primary site of Vtg synthesis in amphipods, as has been established for other crustaceans (Fainzilber et al, 1992;Raviv et al, 2006). In many crustacean species, some Vtg genes are expressed predominantly or even exclusively in the hepatopancreas (eg Chen et al, 1999;Abdu et al, 2002;Tsang et al, 2003;Tsutsui et al, 2004;Mak et al, 2005;Ara and Damrongphol, 2012), however, the qPCR carried out for this study suggests that neither of the two E. marinus Vtg sequences present a bias towards hepatopancreatic expression.…”

Section: Vtg Expression In Normal Male and Female Tissuessupporting

confidence: 91%

Vitellogenin is not an appropriate biomarker of feminisation in a Crustacean

Short¹,

Yang²,

Kille³

et al. 2014

Aquatic Toxicology

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The expression of the yolk protein vitellogenin (Vtg) has been used as a biomarker of feminisation in multiple fish species throughout the world. Since the late 1990s, researchers have attempted to develop similar biomarkers to address whether reproductive endocrine disruption also occurs in the males of invertebrate groups such as the Crustacea. To date, the vast majority of studies investigating Vtg induction in male Crustacea have resulted in negative or inconclusive results, leading researchers to question the utility of Vtg expression as a biomarker in this taxon. This study measured the expression of Vtg genes in two intersex phenotypes (termed internal and external) found in the male amphipod, Echinogammarus marinus, and compared them with those of normal males and females. Males presenting the external intersex phenotype are infected with known feminising parasites and display a variety of feminised traits including oviduct structures on their testes and external female brood plates (oostegites). The internal intersex male phenotype, that displays a pronounced oviduct structure on the testes without the external intersex characteristics, is not parasite infected and it is thought to be a result of environmental contamination. Given their morphology, these phenotypes might be considered highly 'feminised' or 'de-masculinised' and can be utilised to test the suitability of feminisation biomarkers. The E. marinus transcriptome was searched for genes resembling Vtg and two sequences were revealed, that we subsequently refer to as Vtg1 and Vtg2. Results from a high-throughput transcriptomic sequencing screen of gonadal cDNA libraries suggested that very low expression (in this manuscript gene transcription is taken to represent gene expression, although it is acknowledged that in addition to transcription, translation, transcript processing, mRNA stability and protein stability can regulate gene expression) of Vtg1 and Vtg2 in normal males (ESTs=1 and 0 for Vtg1 and Vtg2, respectively), internal intersex males (ESTs=0 for both Vtg sequences) and external intersex males (ESTs=5 and 0 for Vtg1 and Vtg2, respectively). In contrast, the sequencing suggested notable levels of expression of both Vtg genes in females (ESTs=1133 and 84 for Vtg1 and Vtg2, respectively). Subsequent qPCR analysis validates these expression levels, with the signal for Vtg1 and Vtg2 transcripts in all male phenotypes being indistinguishable from that caused by contamination of trace levels of genomic DNA or the low-level amplification non-target sequences. These findings suggest that Vtg expression is not notably induced in highly feminised amphipods and is therefore not an appropriate biomarker of feminisation/de-masculination in crustaceans. We discuss our findings in the context of previous attempts to measure Vtg in male crustaceans and suggest a requirement for more appropriate taxon-specific biomarkers to monitor feminisation in these groups.

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Section: Vtg Expression In Normal Male and Female Tissuessupporting

confidence: 91%

Vitellogenin is not an appropriate biomarker of feminisation in a Crustacean

Short¹,

Yang²,

Kille³

et al. 2014

Aquatic Toxicology

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“…In hepatopancreas, Mn-CL2 maintained high expression level continuously, suggesting that hepatopancreas is the main production site. Previous studies in freshwater prawn and crab species gave proves that Vg was synthesized in hepatopancreas, and then transported to the oocyte through the hemolymph ( 32 , 33 ). The cellular localizations was detected by ISH and Mn-CL2 mRNA were examined in all in five ovary stages.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

et al. 2022

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Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.

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“…VTG of oviparous organisms is sensitive to environmental estrogens and regarded as an environmental indicator to detect environmental pollution by estrogens or estrogenic compounds (Wang et al, 2015). In the study of Ara and Damrongphol (Ara & Damrongphol, 2014), the relative expression level of VTG approximately increased 3-fold after 50 ug/L NP exposure for 4 days. In our study, however, the relative expression level of ERR increased 3 fold after 25 ug/L NP exposure for 6 days, suggesting that the sensitivity of ERR to NP might be better than that of VTG and ERR might be an more excellent environmental indicator.…”

Section: Discussionmentioning

confidence: 99%

“…In mysid Neomysis integer, a low concentration of NP significantly increases the expression of vitellogenin (VTG) (Ghekiere, Verslycke, & Janssen, 2006). VTG expression in the hepatopancreas of the giant freshwater prawn Macrobrachium rosenbergii is also induced by NP in a dose-dependent manner (Ara & Damrongphol, 2014). In vertebrates, the estrogenic effects of NP are mediated by ER (Ashley et al, 2003).…”

Section: Molecular Characterization Of Estrogen-related Receptor (Errmentioning

confidence: 99%

Untitled

Liu¹,

Chen²,

Zhao³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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Nonylphenol (NP) is a xenoestrogen whose estrogenic effects are mediated by the estrogen receptor (ER) in vertebrates. However, the ER appears to be absent in arthropods. Estrogen-related receptor (ERR) may be a candidate that responds to NP. To investigate the effects and mechanisms of NP in giant freshwater prawn Macrobrachium rosenbergii, a unique homolog of mammalian ERR was cloned from M. rosenbergii by rapid amplification of cDNA ends. The full length ERR cDNA consisted of 1897 bp with a 1374 bp open reading frame encoding 457 amino acid residues. RT-PCR analysis revealed that ERR was widely expressed in various tissues of male and female M. rosenbergii, including the brain ganglion, hepatopancreas, heart, and gonads. Interestingly, expression of ERR in ovaries was higher than that in testes. qPCR results showed high expression of ERR in ovaries at early developmental stages. NP exposure up-regulated the expression levels of ERR in the ovaries of M. rosenbergii in a time-dependent manner. The current study provides fundamental data to understand the response of ERR to NP exposure in M. rosenbergii. Aubin, 2001). It has been demonstrated that ERRs are constitutive transcriptional factors that activate expression of target genes via interactions with coactivators in the absence of exogenous ligands (Hong, Yang, & Stallcup, 1999; Schreiber, Knutti, Brogli, Uhlmann, & Kralli, 2003). Although the functions of ERRs are not well understood, they likely play important roles in animal reproduction, growth, development, and metabolism because their expression in embryonic and adult tissues occurs in very restricted spatiotemporal patterns (Giguère, 2002). In mammals, ERRs are involved in ER-dependent pathways related to the formation of bone and cancer development. For

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Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn,Macrobrachium rosenbergii, and stimulation by 4-nonylphenol

Cited by 17 publications

References 27 publications

Vitellogenin is not an appropriate biomarker of feminisation in a Crustacean

Vitellogenin is not an appropriate biomarker of feminisation in a Crustacean

Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

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