Vitamin E-stabilized UHMWPE: Biological response on human osteoblasts to wear debris

Galliera, Emanuela; Ragone, Vincenza; Marazzi, Monica Gioia; Selmin, Francesca; Banci, Lorenzo; Romanelli, Massimiliano Marco Corsi

doi:10.1016/j.cca.2018.07.012

Cited by 29 publications

(25 citation statements)

References 43 publications

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“…Vitamin E has been used to dope UHMWPE in order to provide oxidation resistance upon sterilization, without affecting UHMWPE’s mechanical properties. Galliera et al showed that in bone cells, the vitamin E-blended UHMWPE induced an osteoimmunological response that had a positive effect on the osteolysis induced by wear debris [33].…”

Section: Discussionmentioning

confidence: 99%

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

et al. 2019

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The main objective of this research is to prove the viability of obtaining magnesium (Mg) filled polylactic acid (PLA) biocomposites as filament feedstock for material extrusion-based additive manufacturing (AM). These materials can be used for medical applications, thus benefiting of all the advantages offered by AM technology in terms of design freedom and product customization. Filaments were produced from two PLA + magnesium + vitamin E (α-tocopherol) compositions and then used for manufacturing test samples and ACL (anterior cruciate ligament) screws on a low-cost 3D printer. Filaments and implant screws were characterized using SEM (scanning electron microscopy), FTIR (fourier transform infrared spectrometry), and DSC (differential scanning calorimetry) analysis. Although the filament manufacturing process could not ensure a uniform distribution of Mg particles within the PLA matrix, a good integration was noticed, probably due to the use of vitamin E as a precursor. The results also show that the composite biomaterials can ensure and maintain implant screws structural integrity during the additive manufacturing process.

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Section: Discussionmentioning

confidence: 99%

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

et al. 2019

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“…UHMWPE wear particles were generated as previously described in Galliera et al(26) by four different UHMWPE articular inserts (raw material GUR 1020): Material (1) moderately cross-linked vitamin E-blended UHMWPE (60 kGy electron-beam irradiated) (vitamin E concentration 0.1 wt%), EtO sterilized ( Vital-XE ® , Permedica S.p.A . ); Material (2) standard UHMWPE (without vitamin E and not cross-linked), EtO sterilized (Permedica S.p.A); Material (3) vitamin E-blended UHMWPE (vitamin E concentration 0.1 wt%) not cross-linked, EtO sterilized ( Vital-E ® , Permedica S.p.A .…”

Section: Methodsmentioning

confidence: 99%

“…All UHMWPE wear particles were generated as described previously (26). Briefly, the articular inserts were rubbed for 10 days at 230 rpm against ceramic ball heads using a combined drilling and tapping machine (IM company, Italy), applying a load of 1,000 N. The resulting UHMWPE particles were released directly into a closed sterile recipient containing 500 mL of ultrapure water with 0.2% sodium azide (antibacterial additive).…”

Section: Methodsmentioning

confidence: 99%

Effects of Vitamin E-Stabilized Ultra High Molecular Weight Polyethylene on Oxidative Stress Response and Osteoimmunological Response in Human Osteoblast

Massaccesi

Ragone²,

Papini

et al. 2019

Front. Endocrinol.

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High Crosslink process was introduced in the development of joint prosthetic devices, in order to decrease the wear rate of ultrahigh molecular weight polyethylene (UHMWPE), but it also triggers the formation of free radicals and oxidative stress, which affects the physiological bone remodeling, leading to osteolysis. Vitamin E stabilization of UHMWPE was proposed to provide oxidation resistance without affecting mechanical properties and fatigue strength. The aim of this study is to evaluate the antioxidant effect of vitamin E added to UHMWPE on oxidative stress induced osteolysis, focusing in particular on the oxidative stress response in correlation with the production of osteoimmunological markers, Sclerostin and DKK-1, and the RANKL/OPG ratio compared to conventional UHMWPE wear debris. Human osteoblastic cell line SaOS2 were incubated for 96 h with wear particles derived from crosslinked and not crosslinked Vitamin E-stabilized, UHMWPE without Vitamin E, and growth medium as control. Cellular response to oxidative stress, compared to not treat cells, was evaluated in terms of proteins O-GlcNAcylation, cellular levels of OGA, and OGT proteins by immunoblotting. O-GlcNAcylation and its positive regulator OGT levels are increased in the presence of Vitamin E blended UHMWPE, in particular with not crosslinked Vit E stabilized UHMWPE. Conversely, the negative regulator OGA increased in the presence of UHMWPE not blended with Vitamin E. Vitamin E-stabilized UHMWPE induced a decrease of RANKL/OPG ratio compared to UHMWPE without Vitamin E, and the same effect was observed for Sclerostin, while DKK-1 was not significantly affected. In conclusion, Vitamin E stabilization of UHMWPE increased osteoblast response to oxidative stress, inducing a cellular mechanism aimed at cell survival. Vitamin E antioxidant effect influences the secretion of osteoimmunological factors, shifting the bone turnover balance toward bone protection stimuli. This suggests that Vitamin E-Stabilization of UHMWPE could contribute to reduction of oxidation-induced osteolysis and the consequent loosening of the prosthetic devices, therefore improving the longevity of total joint replacements.

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“…But, as noted, for example, in Scemama et al (2017), long-term observations are still required to guarantee that reduction of XLPE wear will lead to a decrease in the number of cases of osteolysis, leading to aseptic loosening of total hip joint stems. Nevertheless, this direction of total hip joint improving in the scientific environment is considered to be very promising and many studies devoted to it have been carried out recently by various scientific groups (Galliera et al, 2018;Lambert et al, 2019;Massaccesi et al, 2019;Oral et al, 2019;Terkawi et al, 2019;Xu J.-Z. et al, 2019).…”

Section: Improvement Of Materials For Artificial Hip Joint Friction Cmentioning

confidence: 99%

Current Trends in Improving of Artificial Joints Design and Technologies for Their Arthroplasty

2020

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There is a global tendency to rejuvenate joint diseases, and serious diseases such as arthrosis and arthritis develop in 90% of people over 55 years of age. They are accompanied by degradation of cartilage, joint deformities and persistent pain, which leads to limited mobility and a significant deterioration in the quality of life of patients. For the treatment of these diseases in the late stages, depending on the indications, various methods are used, the most radical of which are methods of joint arthroplasty and, in particular, total arthroplasty. Currently, total arthroplasty is one of the most effective and high-quality surgical operations at the relevant medical indications. However, complications may also arise after it, leading, inter alia, to the need for repeated surgical intervention. In order to minimize the likelihood of complications, the artificial joints used in total arthroplasty and the technology of their fabrication are constantly being improved, which leads to the emergence of new designs and methods for their integration with living tissues. At the same time, at the moment, the improvement of traditional designs and production technologies has almost reached the top of their art, and their further improvements can be insignificantly or are associated with the use of the most up-today technologies, allowing for friction couples with low tribological properties to provide for them high ones, for example, gradient increase hardness in the couple titanium alloy on titanium alloy. This paper presents the current state of traditional technical means and technologies for joint arthroplasty. The main attention is paid to the analysis of the latest technologies in the field of joint arthroplasty, such as osseointegration of artificial joints, the improvement of materials with the property of osteoimmunomodulation, the improvement of joint arthroplasty technologies based on the modeling of dynamic osteosynthesis, as well as the identification of possible unconventional designs of artificial joints that contribute to these technologies, predictive assessment of areas for technologies improvement.

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Vitamin E-stabilized UHMWPE: Biological response on human osteoblasts to wear debris

Cited by 29 publications

References 43 publications

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

Effects of Vitamin E-Stabilized Ultra High Molecular Weight Polyethylene on Oxidative Stress Response and Osteoimmunological Response in Human Osteoblast

Current Trends in Improving of Artificial Joints Design and Technologies for Their Arthroplasty

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