Vitamin E protects rat mesenchymal stem cells against hydrogen peroxide-induced oxidative stress in vitro and improves their therapeutic potential in surgically-induced rat model of osteoarthritis

Bhatti, Fazal Ur Rehman; Mehmood, Azra; Latief, Noreen; Zahra, Sana; Cho, Hongsik; Khan, Shaheen N.; Riazuddin, Sheikh

doi:10.1016/j.joca.2016.09.014

Cited by 64 publications

(51 citation statements)

References 49 publications

(49 reference statements)

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“…Moreover, a combination of NAC and L‐ascorbic acid 2‐phosphate promoted the growth of MSCs and suppressed oxidative stress‐induced cell death by enhancing mitochondrial integrity and function in vitro under H 2 O 2 ‐induced oxidative stress . Preconditioning with Vitamin E eliminates H 2 O 2 ‐induced injury in MSCs in vitro, and transplantation of these pretreated MSCs increases the proteoglycan content of cartilage matrix for tissue repair of osteoarthritis in vivo . In addition, metformin can be applied to rescue H 2 O 2 ‐induced senescence, long‐term culture‐induced senescence and hypoxia/serum deprivation (Hy/SD)‐induced apoptosis in MSCs …”

Section: Strategies To Control Ros Levels For Msc Fates In Vitro and mentioning

confidence: 99%

“…54 induced injury in MSCs in vitro, and transplantation of these pretreated MSCs increases the proteoglycan content of cartilage matrix for tissue repair of osteoarthritis in vivo. 94 In addition, metformin can be applied to rescue H 2 O 2 -induced senescence, longterm culture-induced senescence and hypoxia/serum deprivation (Hy/ SD)-induced apoptosis in MSCs. [95][96][97] Intriguingly, fullerol eliminates cellular ROS, effectively retarding the adipogenic differentiation capacity of MSCs while enhancing their osteogenic differentiation capacity.…”

Section: Pharmaceuticals For Ros Regulation In Mscsmentioning

confidence: 99%

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Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Zhao

Peng

et al. 2018

J Cellular Molecular Medi

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Mesenchymal stem cells (MSCs) are broadly used in cell‐based regenerative medicine because of their self‐renewal and multilineage potencies in vitro and in vivo. To ensure sufficient amounts of MSCs for therapeutic purposes, cells are generally cultured in vitro for long‐term expansion or specific terminal differentiation until cell transplantation. Although physiologically up‐regulated reactive oxygen species (ROS) production is essential for maintenance of stem cell activities, abnormally high levels of ROS can harm MSCs both in vitro and in vivo. Overall, additional elucidation of the mechanisms by which physiological and pathological ROS are generated is necessary to better direct MSC fates and improve their therapeutic effects by controlling external ROS levels. In this review, we focus on the currently revealed ROS generation mechanisms and the regulatory routes for controlling their rates of proliferation, survival, senescence, apoptosis, and differentiation. A promising strategy in future regenerative medicine involves regulating ROS generation via various means to augment the therapeutic efficacy of MSCs, thus improving the prognosis of patients with terminal diseases.

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Section: Strategies To Control Ros Levels For Msc Fates In Vitro and mentioning

confidence: 99%

Section: Pharmaceuticals For Ros Regulation In Mscsmentioning

confidence: 99%

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Zhao

Peng

et al. 2018

J Cellular Molecular Medi

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“…11 Hyaline degeneration was reported to be due to cellular apoptosis in rat mesenchymal stem cells. 12 To date, no method to control OIRR has been established. Because lithium inhibits the differentiation of osteoclasts, 2,5,13 lithium may affect OIRR and osteoclast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Lithium chloride reduces orthodontically induced root resorption and affects tooth root movement in rats

Ino-Kondo

Hotokezaka

Kondo

et al. 2018

The Angle Orthodontist

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“…Previous studies have shown that the administration of tocopherol in patients with CKD helped to reduce the risk of cardiovascular complications, increased activity of endogenous antioxidant systems -GPX, catalase [14]. In our previous study [7] we have shown that treatment with an antioxidant lowered the hypoxia-induced cell damage as well as the elevation of HIF-1alpha in vitro [15]. Here we demonstrate that tocopherol administration has a potent effect on hypoxia-induced apoptosis development in children with nephrotic syndrome and subsequent restoration of the proapoptotic factor Bax, BcL-xL and NF-κB activation.…”

Section: Therapeutic Correction Of the Apoptotic Process In Children mentioning

confidence: 78%

Apoptosis correction in nephrotic children

Burlaka

Багдасарова

2019

Ukr. J. Nephrol. and Dial.

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Hypoxia-related disorders play an important role in irreversible kidney tissue damage via activation of certain secondary processes, i.e. apoptosis. This study aimed to investigate the levels of apoptosis controlling factors and nuclear transcriptional factor NF-κB in relation to the value of kidney function impairment; to evaluate possible protective effects of conventional therapy with the addition of an antioxidant agent tocopherol in pediatric patients with nephrotic syndrome. Methods. An examination of blood samples of 53 patients (aged 10 to 15 years) with nephrotic syndrome was performed. Conventional clinical investigations, immunoblotting were used in this study. Results. We demonstrate that nephrotic patients reveal a high level of pro-apoptotic marker Bax, low level of anti-apoptotic factor BcL-xL and anti-apoptotic unit of NF-κB (p65). Their dependence on level of kidney function impairment was detected. Applied treatment composes conventional scheme and tocopherol shows a restored balance of apoptosis controlling factor and NF-κB activity. Conclusion. Hypoxia-induces disorders in nephrotic children resulted in apoptosis controlling system disturbances can be attenuated by the antioxidants application.

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Vitamin E protects rat mesenchymal stem cells against hydrogen peroxide-induced oxidative stress in vitro and improves their therapeutic potential in surgically-induced rat model of osteoarthritis

Abstract: Taken together results of this study demonstrated that Vitamin E pretreated MSCs have an improved ability to impede the progression of OA and thus increased potential to treat OA.

Cited by 64 publications

References 49 publications

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Lithium chloride reduces orthodontically induced root resorption and affects tooth root movement in rats

Apoptosis correction in nephrotic children

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