Lithium chloride reduces orthodontically induced root resorption and affects tooth root movement in rats

Ino-Kondo, Airi; Hotokezaka, Hitoshi; Kondo, Takafumi; Arizono, Keira; Hashimoto, Megumi; Hotokezaka, Yuka; Kurohama, Takeshi; Morita, Yukiko; Yoshida, Noriaki

doi:10.2319/112017-801.1

Cited by 16 publications

(29 citation statements)

References 17 publications

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“…As the concentration of lithium increases, root resorption and tooth movement decrease in animal experiments [38]. As described above, most of the substances administered to reduce root resorption through a mechanism that reduces osteoclast activity have tended to decrease tooth movement speed as well as root resorption [38]. The 4HR used in this study resulted in root resorption being reduced compared to that in the control group (Figures 2 and 3).…”

Section: Discussionmentioning

confidence: 59%

“…Lithium promotes reduced osteoclast formation by promoting the Wnt/β-catenin signaling system [37]. As the concentration of lithium increases, root resorption and tooth movement decrease in animal experiments [38]. As described above, most of the substances administered to reduce root resorption through a mechanism that reduces osteoclast activity have tended to decrease tooth movement speed as well as root resorption [38].…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effects of 4-Hexylresorcinol on Root Resorption Induced by Orthodontic Tooth Movement

Jang

Kim

et al. 2020

Applied Sciences

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Root resorption during orthodontic tooth movement (OTM) is caused by an imbalance between the bone turnover rate and applied mechanical stress. The administration of 4-hexylresorcinol (4HR) increases the bone turnover rate and factors associated with bone formation. Thus, 4HR may show protective activity against root resorption during orthodontic tooth movement (OTM). A total of 40 rats (male: 20; female: 20) were included in this study, and the mandibular first molar was subjected to excessive orthodontic force. The experimental group (n = 20) received 12.8 mg/kg of 4HR every 2 weeks. The controls (n = 20) received a solvent without 4HR. Both groups had the same sex distribution. On Day 28 after the initiation of OTM, all the animals were sacrificed for micro-computed tomography analysis, Western blot analysis, and immunohistochemistry. The ratios of the root length and root volume to the total volume were significantly higher in the experimental group compared to those in the control group (p < 0.05). The expression levels of OPG, RANKL, alkaline phosphatase, and Runx2 in the experimental group according to Western blotting were significantly higher in the experimental group compared to those in the control group (p < 0.05). Their expression was mainly found in the periodontal ligament area. In conclusion, the administration of 4HR decreased the root resorption caused by OTM and increased the expression levels of OPG, RANKL, alkaline phosphatase, and Runx2.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of 4-Hexylresorcinol on Root Resorption Induced by Orthodontic Tooth Movement

Jang

Kim

et al. 2020

Applied Sciences

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“…In support of these findings, previous investigations have demonstrated potential benefits of lithium chloride on fracture healing (26,27) and alveolar bone repair (16)(17)(18)(19)(20)(21). Han et al (16) demonstrated that local administration of lithium chloride stimulated greater alveolar bone volume and mineral density during the healing of surgicallycreated periodontal defects in estrogen-sufficient rats.…”

Section: Discussionmentioning

confidence: 76%

“…Pre-clinical dental studies have shown benefits of lithium chloride, in states of both estrogen sufficiency and deficiency, on bone formation during orthodontic retention, midpalatal expansion and surgically-created periodontal defects, for improving implant osseointegration and for the attenuation of bone loss in a model of induced periodontitis (15)(16)(17)(18)(19)(20). A single previous study in estrogen-sufficient rats evaluated the effects of lithium chloride on the healing process of tooth sockets.…”

Section: Introductionmentioning

confidence: 99%

Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

et al. 2020

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The purpose of this investigation was to evaluate the effects of lithium chloride (LiCl) on the socket healing of estrogen-deficient rats. Seventy-two rats were allocated into one of the following groups: Control, Ovariectomy and LiCl (150 mg/kg/2 every other day orally) + Ovariectomy. Animals received LiCl or water from the 14th day post-ovariectomy, until the completion of the experiment. On the 21st day after ovariectomy, the first molars were extracted. Rats were euthanized on the 10th, 20th and 30th days following extractions. Bone healing (BH), TRAP positive cells and immunohistochemical staining for OPG, RANKL, BSP, OPN and OCN were evaluated. The Ovariectomy group presented decreased BH compared to the LiCl group at 10 days, and the lowest BH at 20 days (p<0.05). At 30 days, the Ovariectomy and LiCl-groups presented lower BH than that of the Control (p<0.05). The number of TRAP-stained cells was the lowest in the LiCl group at 20 days and the highest in the Ovariectomy group at 30 days (p<0.05). At 10 days of healing, the LiCl group demonstrated stronger staining for all bone markers when compared to the other groups, while the Ovariectomy group presented higher RANKL expression than that of the Control (p<0.05). LiCl enhanced bone healing in rats with estrogen deficiency, particularly in the initial healing phases. However, as data on the effects of lithium chloride on bone tissue are still preliminary, more studies related to its toxicity and protocol of administration are necessary before its application in clinical practice.

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“…In recent years, micro CT imaging has been used to investigate root resorption during orthodontic tooth movement for in vivo 16–19 and ex vivo studies 20–22 . For such applications, one of the advantages of using such a method is that the structure of the tooth root can be mapped down to the micrometer level in three dimensions.…”

Section: Introductionmentioning

confidence: 99%

Effect of the software binning and averaging data during microcomputed tomography image acquisition

Friedrichsdorf

Arana-Chavez

Cattaneo

et al. 2019

Sci Rep

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This study describes the effect of the software binning and data averaging during micro CT volume acquisition, on the assessment of root resorption volumes. The mesial roots (n = 9), after orthodontic tooth movement during 14 days, were scanned, using a micro CT system (9 µm/pixel). All roots were reconstructed and the volumes of the resorption lacunae evaluated. The height and width of the pixels vary according to the parameters (A1, A2, A3, A4, A5, A6, A7, A8, A9) used during the scan. In the root #1 the mean volumes of resorption were similar in A4 and A7; in the root #2 there was no similarity in the mean volumes of resorption in any of the parameters; in root #3 only A4 presented mean volume different from zero (3.05 × 10°). In the root #5, the A1 and A7 presented similar mean volumes and in the A6 and A9 presented near mean volumes. In the root #9 the A1, A4, and A7 presented similar mean volumes and A6 and A9 also had similar mean volumes. Significant difference was detected in the volume of resorption among the roots #2, #5 and #9 ( p = 0.04). When analyzing delicate structures such as the roots of rats’ molars, the variation of such parameters will significantly influence the results.

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Lithium chloride reduces orthodontically induced root resorption and affects tooth root movement in rats

Abstract: In rats, LiCl reduced OIRR, which induced mesial movement of the tooth root apex. OIRR positively correlated to cortical bone morphometry.

Cited by 16 publications

References 17 publications

Inhibitory Effects of 4-Hexylresorcinol on Root Resorption Induced by Orthodontic Tooth Movement

Inhibitory Effects of 4-Hexylresorcinol on Root Resorption Induced by Orthodontic Tooth Movement

Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

Effect of the software binning and averaging data during microcomputed tomography image acquisition

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