Vitamin E Inhibits Proliferation of Primary Cultured Human Mesangial and Endothelial Cells

Abstract

Background: Vitamin E (VE) has been used as an antioxidant and has been suggested to inhibit the proliferation of mesangial cells in rat and vascular endothelial cells. The direct effect of VE on primary cultures of mesangial cells (MC) and endothelial cells (EC) from the human glomerulus was studied. Methods: (1) MC (in 17 or 2.5% FCS DMEM) or EC (in 10 or 5% FCS CSC) at 5,000 cells/well was incubated with serial concentrations of VE from 0.05 to 50 µg/ml (0.06 to 60 IU/l). (2) MC was cocultured with 160, 80, 40 or 20 µg/ml of low-density lipoprotein (LDL) or oxidized LDL (ox-LDL) in 17 or 2.5% FCS DMEM with or without VE. After 3 days of incubation at 37°C in 5% CO₂, cell proliferation was measured by the Premix WST-1 Assay System. Results: The concentration of VE that significantly inhibited the proliferation of MC cultured in 17 or 2.5% FCS DMEM was 50 or 2.5 µg/ml (60 or 3.0 IU/l), respectively, and that of EC in 10 or 5% FCS medium was 50 or 25 µg/ml (60 or 30 IU/l). VE at 25 µg/ml (30 IU/l) inhibited the LDL proliferative effect on MC cultured in 2.5 FCS DMEM by 21.79–93.21% in a LDL concentration-dependent manner. There was little difference between the effects of LDL and ox-LDL on the VE inhibitory effect on MC under our experimental conditions. Conclusion: VE at low concentrations had no effect on the proliferation of both MC and EC, but at high concentrations, it showed an inhibitory effect on both cells.

Antioxidants: A Possible Role in Kidney Protection

“…It breaks the propagation of RS chain reaction in the lipids of biological membranes [25]. Vitamin E supplementation inhibits lipoprotein oxidation [68], intraglomerular macrophage infiltration [68] as well as proliferation of mesangial and endothelial cells [74]. Downregulation of renal TGF-ß1 expression was also demonstrated in some reports [75,76], but not in all [77].…”

Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

“…Therefore, we subsequently tried to evaluate the anticancer effect of DTE on cancerous U937 cells. The objective of cell viability assay was to assess the direct growth inhibitory effect of the DTE on the proliferation and survival of the U937 cells [28]. Our results depicted inhibitory effects of DTE on the U937 cells at different time point.…”

Dietary vitamin E affects small intestinal histomorphology, digestive enzyme activity, and the expression of nutrient transporters by inhibiting proliferation of intestinal epithelial cells within jejunum in weaned piglets1

“…Intestinal epithelial cell proliferation has recently been showed to have a positive connection with VH and CD in weaning piglets (Wang et al, 2018). Previous research suggests that VE dosages, within a certain range, inhibit different (Zhang et al, 2001;Tappeiner et al, 2010). No researches illustrate a relationship between VE and jejunal epithelial cell production.…”

“…α-Tocopherol deficiencies affect intestinal transport and the passive absorption of phenolsulfonphthalein and barbital (Meshali et al, 1976). These longknown effects of VE on intestinal functions and more recent studies tending to demonstrate that VE inhibits the in vitro proliferation of various cells (Zhang et al, 2001;Kempnã et al, 2004;Olguín-Martínez et al, 2013) suggested a study of whether, how, and to what extent VE might influence intestinal epithelial cells proliferation in constituting intestinal mucosa, and majorly function as nutrient-absorption (Barker, 2014).…”