Vitamin E Improves Biochemical Indices Associated with Symptoms of Atopic Dermatitis-Like Inflammation in NC/Nga Mice

Abstract

Summary We aimed to define whether vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice. After picryl chloride (PC) application to their backs, changes in the content of thiobarbituric acid reactive substances (TBARS) and vitamin E, as well as the activity of antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase) were analyzed in the serum and skin of NC/Nga mice during a symptomatic cycle. The levels of inflammatory factors were also assessed, including IgE, cyclooxigenase-2 (COX-2), tumor necrosis factor (TNF-␣ ) and nuclear factor-B (NF-B). When allergic dermatitis was induced by the application of PC to the skin of the mice, skin inflammation appeared 2 wk after PC application, with the peak severity of inflammation observed 5 wk after PC application. Subsequently, the animals recovered from the inflammation by 9 wk after PC application. The TBARS content in the skin and serum increased markedly when the symptoms were the most severe, and decreased to levels near those in control mice by 9 wk after PC application. The activities of SOD and GSHPx in the skin and serum were also positively correlated with symptomatic changes; however, no change in catalase activity was observed 5 wk after PC application. Conversely, vitamin E content decreased at the stage of peak severity. The levels of all inflammatory factors analyzed in this study were altered in a manner similar to other indices. Additionally, vitamin E treatment markedly inhibited these PC-induced alterations. On the basis of these results, it is expected that the observed alterations in biochemical indices, which reflect the symptomatic cycle, may be applicable to objective diagnosis and treatment for atopic dermatitis, and that vitamin E may improve the symptoms of AD.

Extracellular superoxide dismutase for the treatment of inflammatory skin diseases

“…Additionally, ROS has been shown to stimulate the inflammasome, promoting the maturation of proinflammatory cytokines such as IL-1b [27,28]. Consistent with these observations, many anti-oxidants, such as natural flavonoids isolated from herbal medicines (vitamin C, vitamin A, vitamin E and N-acetyl cysteine) and anti-oxidant enzymes, have been shown to alleviate inflammatory responses [29][30][31][32][33][34][35][36], supporting ROS as a promising target for treating chronic inflammatory diseases. Here, we review about one of cellular anti-oxidant enzyme, extracellular superoxide dismutase (SOD3) as a potential biomedicine and its application for treatment against inflammatory diseases.…”

Topical Apigenin Alleviates Cutaneous Inflammation in Murine Models

“…Studies have demonstrated that antioxidants such as quercetin (Que) and cromolyn inhibit release of inflammatory mediators including histamine, leukotrienes, IL-6, IL-8, and TNF release from mast cells in vitro [48]. In vivo studies reveal that vitamin E, an antioxidant, improves atopic dermatitis-like inflammation [49]. Therefore, the anti-inflammatory effect of apigenin could be due to its antioxidant effects.…”

Vitamin E in Atopic Dermatitis: From Preclinical to Clinical Studies

“…The role of vitamin E in relieving inflammation and erythema was studied by Hayashi et al [16] using an NC/Nga mouse model. In their study, mice were subcutaneously injected in their backs with a 4% α-TP (200 mg/kg body mass) every other day for 2 weeks.…”

“…T3 has been shown to display a superior antioxidant effect as compared to TP due to its unsaturated and isoprenoid side chain [13-15]. Apart from its antioxidant activity, vitamin E is able to suppress inflammation and upregulate gene expression of keratinocyte differentiation markers, suggesting its potential as a therapeutic agent against AD [16, 17].…”