Topical Apigenin Alleviates Cutaneous Inflammation in Murine Models

Man, Mao‐Qiang; Hupe, Melanie; Sun, Richard; Man, George; Mauro, Theodora M.; Elias, Peter M.

doi:10.1155/2012/912028

Cited by 40 publications

(34 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…pH900 was used to measure skin surface pH. For barrier recovery, TEWL was measured at 0, 2 and 4 h after tape stripping (10‐fold increase in TEWL), and percentage barrier recovery was calculated as described earlier .…”

Section: Methodsmentioning

confidence: 99%

Topical hesperidin prevents glucocorticoid‐induced abnormalities in epidermal barrier function in murine skin

Man

Mauro

Kim

et al. 2014

Experimental Dermatology

Self Cite

View full text Add to dashboard Cite

Systemic and topical glucocorticoids (GC) can cause significant adverse effects not only on the dermis, but also on epidermal structure and function. In epidermis, a striking GC-induced alteration in permeability barrier function occurs that can be attributed to an inhibition of epidermal mitogenesis, differentiation and lipid production. As prior studies in normal hairless mice demonstrated that topical applications of a flavonoid ingredient found in citrus, hesperidin, improve epidermal barrier function by stimulating epidermal proliferation and differentiation, we assessed here whether its topical applications could prevent GC-induced changes in epidermal function in murine skin and the basis for such effects. When hairless mice were co-treated topically with GC and 2% hesperidin twice-daily for 9 days, hesperidin co-applications prevented the expected GC-induced impairments of epidermal permeability barrier homoeostasis and stratum corneum (SC) acidification. These preventive effects could be attributed to a significant increase in filaggrin expression, enhanced epidermal β-glucocerebrosidase activity and accelerated lamellar bilayer maturation, the last two likely attributable to a hesperidin-induced reduction in stratum corneum pH. Furthermore, co-applications of hesperidin with GC largely prevented the expected GC-induced inhibition of epidermal proliferation. Finally, topical hesperidin increased epidermal glutathione reductase mRNA expression, which could counteract multiple functional negative effects of GC on epidermis. Together, these results show that topical hesperidin prevents GC-induced epidermal side effects by divergent mechanisms.

show abstract

Section: Methodsmentioning

confidence: 99%

Topical hesperidin prevents glucocorticoid‐induced abnormalities in epidermal barrier function in murine skin

Man

Mauro

Kim

et al. 2014

Experimental Dermatology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, botanical ingredients are increasingly being added to moisturizers, and some of these can be beneficial. For example, chamomile contains anti-inflammatory substances, such as apigenin, which can improve AD symptoms [6]. …”

Section: Moisturizers Do Not Equate With Barrier Repair Therapymentioning

confidence: 99%

Moisturizers versus Current and Next-Generation Barrier Repair Therapy for the Management of Atopic Dermatitis

Elias¹,

Wakefield²,

Man³

2018

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

We compare here the principal characteristics of over-the-counter moisturizers with physiologic lipid-based barrier repair therapy. Moisturizers are standard ancillary therapy for anti-inflammatory skin disorders, like atopic dermatitis (AD), and can attenuate the emergence of AD, the initial step in the “atopic march.” But not all moisturizers are beneficial; some can make skin function worse, and can even induce inflammation, possibly accounting for the frequent occurrence of “sensitive skin” in women. In contrast, physiologic lipid-based barrier repair therapy, if comprised of the 3 key stratum corneum lipids, in sufficient quantities and at an appropriate molar ratio, can correct the barrier abnormality and reduce inflammation in AD, and perhaps in other inflammatory dermatoses.

show abstract

“…In the recent past, several studies reported the mitigation of matrix metalloproteinases (MMPs) expression by apigenin in target cells, which is induced by several agents such as carcinogens, ultraviolet A (UVA 320–400 nm), phorbol myristate acetate (PMA), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) [18]–[21]. Further, inhibitory action of apigenin or apigenin structural analogues against cutaneous inflammation and infection-induced inflammation is also demonstrated [22], [23]. However, no study claims the SVMP inhibitory efficacy of apigenin or derivatives having apigenin nucleus.…”

Section: Introductionmentioning

confidence: 99%

Novel Apigenin Based Small Molecule that Targets Snake Venom Metalloproteases

et al. 2014

View full text Add to dashboard Cite

The classical antivenom therapy has appreciably reduced snakebite mortality rate and thus is the only savior drug available. Unfortunately, it considerably fails to shield the viper bite complications like hemorrhage, local tissue degradation and necrosis responsible for severe morbidity. Moreover, the therapy is also tagged with limitations including anaphylaxis, serum sickness and poor availability. Over the last decade, snake venom metalloproteases (SVMPs) are reported to be the primary component responsible for hemorrhage and tissue degradation at bitten site. Thus, antivenom inability to offset viper venom-induced local toxicity has been a basis for an insistent search for SVMP inhibitors. Here we report the inhibitory effect of compound 5d, an apigenin based molecule against SVMPs both in silico and in vivo. Several apigenin analogues are synthesized using multicomponent Ugi reactions. Among them, compound 5d effectively abrogated Echis carinatus (EC) venom-induced local hemorrhage, tissue necrosis and myotoxicity in a dose dependant fashion. The histopathological study further conferred effective inhibition of basement membrane degradation, and accumulation of inflammatory leucocytes at the site of EC venom inoculation. The compound also protected EC venom-induced fibrin and fibrinogen degradation. The molecular docking of compound 5d and bothropasin demonstrated the direct interaction of hydroxyl group of compound with Glu146 present in hydrophobic pocket of active site and does not chelate Zn2+. Hence, it is concluded that compound 5d could be a potent agent in viper bite management.

show abstract

Topical Apigenin Alleviates Cutaneous Inflammation in Murine Models

Cited by 40 publications

References 50 publications

Topical hesperidin prevents glucocorticoid‐induced abnormalities in epidermal barrier function in murine skin

Topical hesperidin prevents glucocorticoid‐induced abnormalities in epidermal barrier function in murine skin

Moisturizers versus Current and Next-Generation Barrier Repair Therapy for the Management of Atopic Dermatitis

Novel Apigenin Based Small Molecule that Targets Snake Venom Metalloproteases

Contact Info

Product

Resources

About