Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model

Jeremy, Malsawmhriatzuala; Gurusubramanian, Guruswami; Roy, Vikas Kumar

doi:10.1016/j.jsbmb.2019.03.016

Cited by 22 publications

(21 citation statements)

References 56 publications

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“…There are now evidence that that D ‐gal treatment also causing the reproductive aging, characterized by decreased sperm count, testis weight, serum testosterone, and LH levels (Ahangarpour et al, ; Liao et al, ; Yang et al, ). We have shown that treatment of vitamin D3 ameliorated the D ‐gal induced aged testicular activity (Jeremy et al, ). AGE exerts biological effects by acting through cell surface receptor, AGER ( Howes et al, ) .…”

Section: Discussionmentioning

confidence: 98%

“…D ‐gal treatment was given to the rat to show reproductive alterations compatible with reproductive aging. The doses (40 IU/Kg and 400 IU/Kg) and duration (6 weeks, twice weekly) of vitamin D3 injections were given intramuscularly (im) as per our previous study (Jeremy et al, ) .…”

Section: Methodsmentioning

confidence: 99%

“…Membranes were then rinsed with TBST two times and immersed in enhanced chemiluminescence (ECL; Cat # 1705060, BioRad, Hercules, CA) for 90 s. After draining excess ECL buffer, the bands were developed onto X‐ray film and quantified using the ImageJ software. A separate probe was developed for β‐Tubulin for loading control (Jeremy et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…However, there is no comparative report on the reproductive parameters between D ‐gal treated animal with natural age. Recently our laboratory has also shown that D ‐gal treatment to rat showed decreased sperm parameters (Jeremy, Gurusubramanian, & Roy, ; Jeremy, Gurusubramanian, & Roy, ).…”

Section: Introductionmentioning

confidence: 93%

“…The role of vitamin D has been well documented in mammalian aging ( Lanske & Razzaque, ) and it has also been advocated that vitamin D has potential to slow down aging ( Meehan & Penckofer, ) . Recently we have shown that treatment of vitamin D to aged rat delay testicular aging by modulating the steroidogenesis ( Jeremy et al, ) . As aging is a multifactorial process, thus it is logical to hypothesize that there would be various regulatory molecules involved in male aging, which might be affecting epididymal sperm during aging.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

Jeremy

Gurusubramanian

Roy

2019

Molecular Reproduction Devel

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The present study aimed to investigate the effects of vitamin D3 in the epididymal sperm cells of D‐gal‐induced aged rats. It is well known that during aging sperm quality and quantity declines and leads to age‐related infertility problems in males. The results of the present study showed that there were elevated levels of oxidative stress and poor DNA integrity of sperm of aged rats. The expression of BCL2 also showed a significant decline in the sperm of aged rats, however, the expression of BAX and active caspase‐3 did not show significant change compared with the control group. The treatment of vitamin D3 at lower doses to aged rats showed increased expression of BAX and active caspase‐3 in the sperm, this finding suggests that increased apoptosis may be responsible for removal of poor quality sperm during aging. Vitamin D3 treatment at both doses showed improvement in the oxidative stress and DNA integrity in the sperm of aged rats. We also investigated the expression of AGER, visfatin, and HSPA1A in the epididymal sperm. It has been found that expression of AGER, visfatin, and HSPA1A increased in the sperm aged rats and vitamin D3 treatments at both doses decreased its expression. Thus, it might be suggested that during aging vitamin D3 treatment would be important for managing the sperm quality by regulating the apoptosis, antioxidant system and DNA integrity via modulation of visfatin and HSPA1A.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

Jeremy

Gurusubramanian

Roy

2019

Molecular Reproduction Devel

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1α,25‐dihydroxyvitamin D₃ supplementation alleviates perfluorooctanesulfonate acid‐induced reproductive injury in male mice: Modulation of Nrf2 mediated oxidative stress response

Liang

et al. 2022

Environmental Toxicology

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Perfluorooctanesulfonate acid (PFOS) is a typical persistent organic pollutant that widely exists in the environment. To clarify the toxic effects and mechanisms of PFOS and to find effective intervention strategies have been attracted global attention. Here, we investigated the effects of PFOS on the male reproductive system and explored the potential protective role of 1α,25-dihydroxyvitamin D 3 (1α,25(OH) 2 D 3 ).Our results showed that 1α,25(OH) 2 D 3 intervention significantly improved PFOSinduced sperm quality decline and testicular damage. Moreover, 1α,25(OH) 2 D 3 aggrandized the total antioxidant capacity. Furthermore, after PFOS exposure, the transcription factor nuclear factor erythroid-related factor 2 (Nrf2) was adaptively increased together with its target genes, such as HO-1, NQO1, and SOD2. Meanwhile, 1α,25(OH) 2 D 3 ameliorated PFOS-induced augment of Nrf2 and target genes.These findings indicated that 1α,25(OH) 2 D 3 might attenuate PFOS-induced reproductive injury in male mice via Nrf2-mediated oxidative stress. K E Y W O R D S 1α,25(OH) 2 D 3 , Nrf2, oxidative stress, perfluorooctanesulfonate acid, reproductive toxicity 1 | INTRODUCTION Perfluorooctanesulfonic acid (PFOS) is a representative synthetic organo-fluorine compound, which has been widely used as a stain repellent and anti-stick material due to its excellent hydrophobic and lipophobic properties. 1 However, PFOS is difficult to degrade and widely exists in water, dust, soil, and other environmental media. 2,3 Noteworthy, PFOS generates a bioaccumulation effect. Drinking water, contaminated food consumption, product usage, and occupational exposure are the main approaches to PFOS exposure. PFOS has been extensively detected in human blood, umbilical cord blood, breast milk, and other samples worldwide. 4 Therefore, PFOS exposure has become a global environmental problem, and it is of great importance to clarify the toxic effects and mechanisms of PFOS and to find effective intervention strategies.In human body, PFOS combines with proteins, such as liver fatty acid transporters in the liver and albumin in the plasma. Liver is the primary target of PFOS, which can cause typical hepatic toxicity by disrupting glucose and lipid metabolism. [5][6][7][8] Recent studies have discovered that PFOS can also induce immune toxicity, 9 cardiotoxicity, 10 and neurotoxicity. 11 Furthermore, PFOS has significant reproductive

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Differential effect of visfatin inhibition on the testicular androgen and estrogen receptors expression in early pubertal mice

Rempuia,

Gurusubramanian,

Roy

2024

Endocrine

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Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model

Cited by 22 publications

References 56 publications

Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

1α,25‐dihydroxyvitamin D₃ supplementation alleviates perfluorooctanesulfonate acid‐induced reproductive injury in male mice: Modulation of Nrf2 mediated oxidative stress response

Differential effect of visfatin inhibition on the testicular androgen and estrogen receptors expression in early pubertal mice

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Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model

Cited by 22 publications

References 56 publications

Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

Vitamin D3 treatment regulates apoptosis, antioxidant defense system, and DNA integrity in the epididymal sperm of an aged rat model

1α,25‐dihydroxyvitamin D3 supplementation alleviates perfluorooctanesulfonate acid‐induced reproductive injury in male mice: Modulation of Nrf2 mediated oxidative stress response

Differential effect of visfatin inhibition on the testicular androgen and estrogen receptors expression in early pubertal mice

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1α,25‐dihydroxyvitamin D₃ supplementation alleviates perfluorooctanesulfonate acid‐induced reproductive injury in male mice: Modulation of Nrf2 mediated oxidative stress response