Vitamin C Supplementation Does Not Modify Bone Mineral Content or Mineral Absorption in Growing Pigs ,

Pointillart, Alain; Denis, Isabelle; Colin, Colette; Lacroix, H.

doi:10.1093/jn/127.8.1514

Cited by 23 publications

(21 citation statements)

References 20 publications

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“…In the present study, there was no significant difference in Ca absorption between the CH and CHVC groups. These findings are in accordance with previous results that Ca and P balances in pigs on a diet supplemented with vitamin C (1,000mg/kg) did not differ from those of pigs fed a control diet, and also that vitamin C supplementation had no effect on the plasma concentration of Ca, iP or 1,25(OH)2D3 (25). Thus, decreased serum Ca and increased serum P as well as the 1,25(OH)2D3 level in the CHVC group were most likely induced not by vitamin C supplementation but by chitosan ingestion.…”

Section: Discussionsupporting

confidence: 93%

Effects of Habitual Chitosan Intake on Bone Mass, Bone-Related Metabolic Markers and Duodenum CaBP D9K mRNA in Ovariectomized SHRSP Rats.

Yang

Nakajima

et al. 2002

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryWe have demonstrated that the habitual intake of chitosan can decrease bone mass in ovariectomized (OVX) SHRSP rats fed a low-Ca diet (0.1%). In the present study, we examined both the etiology of bone loss induced by dietary chitosan and the preventive ef fect of vitamin C supplementation. Rats were OVX and maintained on one of the following diets for 6 wk: 10% cellulose (CE), 10% chitosan (CH) or 10% chitosan with sodium ascor bate (CHVC). CH caused a significant reduction in bone mineral density (BMD) and stiffness in femurs and the fourth lumbar vertebrae (L4). There was no significant difference in intes tinal Ca absorption between CH and CE, whereas CH intake significantly reduced intestinal P absorption. The bone loss in CH rats was accompanied with an increase in urinary Ca ex cretion and a decrease in serum Ca as well as a significant increment in serum PTH and 1,25(OH)2D3. The vitamin D receptor and calcium binding protein D9K mRNAs were also significantly increased in the duodenum of CH rats. Vitamin C supplementation to CH caused an increase in the Ca and P contents of femurs as well as BMD of the L4, with a de crease in urinary Ca excretion. These results indicate that dietary chitosan with low Ca in take possibly induces the loss of bone mass by enhancing urinary Ca excretion rather than by inhibiting Ca absorption, and that vitamin C supplementation could prevent bone loss caused by chitosan through the increment of retained Ca followed by suppression of urinary Ca excretion. Key Words dietary chitosan, OVX rats, bone loss, serum 1,25(OH)2D3, CaBP D9K mRNA Chitosan, a polymer of glucosamine and a type of water insoluble dietary fiber, is prepared by the deacety lation of chitin, which constitutes the exoskeletons of arthropods such as crabs, shrimp, lobsters and insects (1). Chitosan has been shown to have a hypocholes terolemic effect in humans and animals (2-5), and also an inhibitory effect on intestinal fat digestion and ab sorption in rats (6). It has also been reported to be of low toxicity (7), and has come to be widely used as a food supplement to lower blood cholesterol and prevent weight gain. However, in spite of a number of nutri tional advantages, chitosan may serve as a chelator of several minerals and hence impair their bioavailabili ties. Deuchi et al. discovered that decreased intestinal absorption of calcium (Ca) and bone mineral contents of femurs were induced in male rats when fed diets con taining 5% chitosan for 2 wk (8). Moreover, Wada et al., in an experiment using 47Ca, observed that there was no discriminatory difference in fecal Ca excretion in rats fed chitosan or cellulose, but a significant increase in urinary Ca excretion with chitosan intake (9). These studies suggested that dietary chitosan might influence calcium and bone metabolism through inhibiting Ca absorption and/or by accelerating its urinary excretion.The decrease in net Ca retention as a consequence of habitual intake of chitosan with a low-Ca diet may en hance the risk of osteopenia or osteoporosi...

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Section: Discussionsupporting

confidence: 93%

Effects of Habitual Chitosan Intake on Bone Mass, Bone-Related Metabolic Markers and Duodenum CaBP D9K mRNA in Ovariectomized SHRSP Rats.

Yang

Nakajima

et al. 2002

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Little information is available from previous experiments. Avci et al (2005) and Pointillart et al (1997) reported no effect of vitamin C on different electrolytes on Japanese quail and growing pigs, respectively.…”

Section: Blood Electrolytes Ph and Cortisolmentioning

confidence: 99%

“…Vitamin C supplementation seems to be beneficial for enhancing growth and performance of weaned pigs, particularly at a very young age (de Rodas et al, 1998;Mahan and Saif, 1983;Yen and Pond, 1981). Furthermore, studies on swine have shown that vitamin C supplementation had no positive effect on bone metabolism (Pointillart et al, 1997), nor did it affect blood hemoglobin and hematocrit levels (de Rodas et al, 1998;Mahan and Saif, 1983).…”

Section: Article In Pressmentioning

confidence: 99%

Physiological and chemical responses in water-deprived Awassi ewes treated with vitamin C

Ghanem

Jaber

Said

et al. 2008

Journal of Arid Environments

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“…Bone mineral density (BMD), in grams per square centimeter, and bone mineral contents (BMC), in grams, were measured P, total and bone ALP were measured as previously described [39,40]. PICP was determined by radioimmunoassay (Orion Diagnostica, Espoo, Finland).…”

Section: Bone Mineral Densitymentioning

confidence: 99%

“…A 5% sample of each 24 h urine collection was taken and the samples were pooled for the 10 days. Calcium and deoxypyridinoline were determined on these urine samples [40]. The concentration of deoxypyridinoline was measured using a competitive enzyme immunoassay (PYRILINKS-D, Metra Biosystems from Behring Diagnostic, Rueil-Malmaison, France).…”

Section: Urinary Parametersmentioning

confidence: 99%

Availability of calcium from skim milk, calcium sulfate and calcium carbonate for bone mineralization in pigs

Pointillart¹,

Coxam²,

Sève

et al. 2000

Reprod. Nutr. Dev.

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-Dairy products provide abundant, accessible calcium for humans, while some calcium sulfate-rich mineral waters could provide appreciable amounts of calcium. But there is little evidence that this calcium is as available as milk calcium for making bone. The availability of calcium was studied by monitoring bone parameters in 2-month-old pigs fed restricted amounts of calcium (70% RDA) for 2.5 months. The 3 main (≥ 50% Ca intake) Ca sources were either CaCO 3 or CaSO 4 or skim milk powder (29% of the diet). The bones of the pigs fed the "milk" diet had higher (P < 0.01) ash contents, breaking strength and density (DEXA) than those of the two others groups, in which the bone values were similar. Thus, the calcium provided by a diet containing milk appears to ensure better bone mineralization than do calcium salts included in a non-milk diet. The calcium restriction may have enhanced some milk properties to stimulate calcium absorption in these young, rapidly growing pigs.calcium availability / calcium sulfate / milk calcium / bones / pigs Résumé -Biodisponibilité comparée du calcium du lait, du sulfate et du carbonate de calcium pour la minéralisation osseuse: étude chez le porc. Le sulfate de calcium, dont certaines eaux minérales sont riches, est-il aussi bien utilisé pour la minéralisation osseuse que le calcium des produits laitiers ? Trois lots de 8 porcs, de 2 mois, recevaient pendant 2,5 mois, trois régimes dont au moins 50 % de l'apport calcique était fourni par CaSO 4 , CaCO 3 ou la poudre de lait. Bien que différents dans leur composition, les trois régimes étaient formulés pour avoir la même valeur nutritionnelle et leur teneur en Ca était restreinte (70 % des recommandations). Avec le régime « lait », les cendres, la résistance à la rupture et la densité minérale des os étaient supérieures à celles des deux autres groupes, non différents l'un de l'autre. L'analyse des paramètres circulants liés au métabolisme osseux (en particulier au collagène), des 3 groupes, exclut l'hypothèse d'un effet direct de la source Reprod. Nutr. Dev. 40 (2000) 49-61 49

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Vitamin C Supplementation Does Not Modify Bone Mineral Content or Mineral Absorption in Growing Pigs ,

Cited by 23 publications

References 20 publications

Effects of Habitual Chitosan Intake on Bone Mass, Bone-Related Metabolic Markers and Duodenum CaBP D9K mRNA in Ovariectomized SHRSP Rats.

Effects of Habitual Chitosan Intake on Bone Mass, Bone-Related Metabolic Markers and Duodenum CaBP D9K mRNA in Ovariectomized SHRSP Rats.

Physiological and chemical responses in water-deprived Awassi ewes treated with vitamin C

Availability of calcium from skim milk, calcium sulfate and calcium carbonate for bone mineralization in pigs

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