Vitamin B6 prevents excessive inflammation by reducing accumulation of sphingosine‐1‐phosphate in a sphingosine‐1‐phosphate lyase–dependent manner

Du, Xialin; Yang, Yalong; Zhan, Xiaozhi; Huang, Yulan; Fu, Yuling; Zhang, Zelin; Liu, Honglin; Zhang, Lijie; Li, Yanfen; Wen, Qian; Zhou, Xinying; Zuo, Daming; Zhou, Chaoying; Li, Laisheng; Hu, Shengfeng; Ma, Li

doi:10.1111/jcmm.15917

Cited by 26 publications

(20 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The microflora residing in Landrace pigs was significantly correlated with Staphylococcus aureus infections, and the risk of streptococcal infection was high. In contrast, the bacterial communities of Tibetan pigs was vigorously correlated with vitamin B1, B5, and B6 metabolism and synthesis, which facilitated to strengthen its immune system, especially anti-inflammatory responses ( Du et al, 2020 ). The NOD-like receptor was a key factor involved in the regulation of the host’s native immune responses and was identified to be significantly correlated with the bacteria in Tibetan pigs.…”

Section: Discussionmentioning

confidence: 99%

Gut Bacterial Composition and Functional Potential of Tibetan Pigs Under Semi-Grazing

et al. 2022

View full text Add to dashboard Cite

Gut bacterial community plays a key role in maintaining host health. The Tibetan pig (Sus scrofa), an ancient breed in China, has been known for its high adaptability to harsh environments and for its meat quality. To understand the underlying mechanisms facilitating to shape these unique features, in this study, 16S rRNA sequencing using pigs feces and subsequent bacterial functional prediction were performed. Also, the gut bacteria of two other breeds of pigs, Barkshire and Landrace, were examined for comparison. It was revealed that the structure of bacterial community in Tibetan pigs appeared to be more complex; the relative abundances of dominant bacterial families varied inversely with those of the other pigs, and the proportion of Firmicutes in Tibetan pigs was lower, but Bacteroides, Fibrobacterota, Lachnospiraceae, Oscillospiraceae, and Ruminococcaceae were higher. Bacterial functional prediction revealed that the dominant flora in the Tibetan pigs was more correlated with functions regulating the hosts’ immune and inflammatory responses, such as NOD-like_receptor_signaling_pathway and vitamin metabolism. In addition, in Tibetan pigs, the taxonomic relationships in the gut bacteria on day 350 were closer than those on earlier stages. Furthermore, gender played a role in the composition and function of bacterial inhabitants in the gut; for boars, they were more correlated to drug resistance and xenobiotics metabolism of the host compared to the sows. In sum, our preliminary study on the gut bacterial composition of the Tibetan pigs provided an insight into the underlying host–microorganism interactions, emphasizing the role of intestinal bacteria in the context of modulating the host’s immune system and host development.

show abstract

Section: Discussionmentioning

confidence: 99%

Gut Bacterial Composition and Functional Potential of Tibetan Pigs Under Semi-Grazing

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Furthermore, our study did not show any positive effect on the inflammatory markers after vitamin B6 treatment. Although several studies support the anti-inflammatory effect of vitamin B6 in different inflammatory conditions [ 31 ], this effect may be attenuated in the low-grade or central inflammation situations such as FM. The purported neuroinflammation and low-grade systemic inflammation in FM may not be detectable by usual methods.…”

Section: Discussionmentioning

confidence: 99%

Effect of vitamin B6 on pain, disease severity, and psychological profile of fibromyalgia patients; a randomized, double-blinded clinical trial

Ghavidel‐Parsa

Naeimi

Gharibpoor

et al. 2022

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background Given the role of vitamin B6 on pronociceptive/antinociceptive neurotransmitters balance, metabolic reactions, and inflammation, it is important to clarify the effect of vitamin B6 on pain and psychological disturbance in fibromyalgia (FM). This study aimed to evaluate whether an 80-mg daily dose of vitamin B6 improves pain, disease severity and psychological symptoms of FM compared to a placebo. Methods This randomized, double-blinded, placebo-controlled trial was performed on the FM patients whose diagnosis was confirmed by a rheumatologist based on the 2016 American College of Rheumatology (ACR). 90 Patients were randomized to receive either vitamin B6 (80 mg daily) or placebo in a 1:1 ratio, with a permuted block size of 30 stratified by disease severity. Primary outcomes included the Revised Fibromyalgia Impact Questionnaire (FIQR), Hospital Anxiety and Depression Scale (HADS), 12-item short-form health survey (SF-12), and pain visual analog scale (pain-VAS)). The mean differences in outcomes (before and after treatment) were compared between the vitamin B6 and placebo groups using an independent T-test. An ANCOVA model adjusted for baseline outcome value was also provided to compare the outcomes between the two groups. Results Of 90 eligible patients, 60 patients (31 patients in vitamin B6 and 29 in the placebo group) completed the trial. Overall, the FIQR, pain-VAS, and HADS-anxiety scores improved after treatment in both vitamin B6 and placebo groups; However, there was no statistically significant intergroup difference regarding primary outcomes. ANCOVA model also showed no difference in the treatment effects. Conclusions Our results showed no priority for vitamin B6 over placebo in FM patients. Considering the potential ameliorating role of vitamin B6 on pain and psychological symptoms, acknowledgment of vitamin B6 as a relatively safe adjuvant treatment needs larger future studies. Trial registration Iranian Registry of Clinical Trials: IRCT20200920048782N2 on 2021/10/04.

show abstract

“…injected with LPS (5 mg/kg, from E. coli 0111:B4, Sigma-Aldrich, USA) with or without miR-26a mimic (Ribo Bio, China). For miR-26a mimic-treated mice, the mimics dose (100 μL/mouse) was equivalent to 10 mg miR-26a/kg body weight on day one according to a previous report [ 25 ]. The miR-26a mimic and LPS were dissolved in 0.9% normal saline for in vivo experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The lethal endotoxic shock was induced in C57BL/6J mice by i.p. LPS injection (10 mg/kg) according to a previous report [ 25 ]. The death of mice was judged according to whether the mice had a heartbeat or not.…”

Section: Methodsmentioning

confidence: 99%

MiR-26a Reduces Inflammatory Responses via Inhibition of PGE2 Production by Targeting COX-2

Wang

et al. 2022

Inflammation

Self Cite

View full text Add to dashboard Cite

MicroRNAs are small non-coding RNA regulatory molecules that play an important role in the development and function of immune cells. MicroRNA-26a (miR-26a) exhibits anti-inflammatory immune effects on immune cells. However, the exact mechanism by which miR-26a plays an anti-inflammatory role remains unclear. Here, we report that miR-26a reduces inflammatory response via inhibition of prostaglandin E2 (PGE2) production by targeting cyclooxygenase-2 (COX-2). We found that miR-26a was downregulated in vitro and in vivo . The miR-26a mimic significantly decreased COX-2 protein levels, further inhibiting pro-inflammatory cytokine production in LPS-stimulated macrophages. We predicted that miR-26a could potentially target COX-2 in LPS-stimulated macrophages. Computational algorithms showed that the 3′-UTR of COX-2 mRNA contains a binding site for miR-26a. This putative targeting relationship between miR-26a and COX-2 was further confirmed by a dual-reporter gene assay. The anti-inflammatory effects of the miR-26a mimic were diminished by PGE2 supplementation. Importantly, miR-26a mimics protected mice from lethal endotoxic shock and attenuated pro-inflammatory cytokine production. Collectively, these results suggest that miR-26a may function as a novel feedback negative regulator of the hyperinflammatory response and as a drug target for the progression of inflammation. Supplementary Information The online version contains supplementary material available at 10.1007/s10753-022-01631-2.

show abstract

Vitamin B6 prevents excessive inflammation by reducing accumulation of sphingosine‐1‐phosphate in a sphingosine‐1‐phosphate lyase–dependent manner

Cited by 26 publications

References 39 publications

Gut Bacterial Composition and Functional Potential of Tibetan Pigs Under Semi-Grazing

Gut Bacterial Composition and Functional Potential of Tibetan Pigs Under Semi-Grazing

Effect of vitamin B6 on pain, disease severity, and psychological profile of fibromyalgia patients; a randomized, double-blinded clinical trial

MiR-26a Reduces Inflammatory Responses via Inhibition of PGE2 Production by Targeting COX-2

Contact Info

Product

Resources

About