Vitamin B2 production by Acetobacterium sp. and its tetrachloromethane-resistant mutants

Inoue, Kazuhiro; Kageyama, Sadao; Miki, Katsuya; Morinaga, Tsuyoshi; Kamagata, Yoichi; Nakamura, Kazunori; Mikami, Eiichi

doi:10.1016/0922-338x(92)90238-p

Cited by 24 publications

(8 citation statements)

References 12 publications

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“…Acetobacterium spp. are known for vitamin B 12 production (2,3,26,45); however, the ability of Dehalobacter to synthesize or salvage corrinoids is unknown. More work is required to determine how the WL Acetobacterium culture is in fact assisting Dehalobacter, if at all.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene

Grostern¹,

Edwards

2009

Appl Environ Microbiol

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Dehalobacter and "Dehalococcoides" spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a Dehalobacter sp. from this mixed culture in coculture with an Acetobacterium sp. Through a series of serial transfers and dilutions with acetate, H 2 , and 1,2-DCA, a stable coculture of Acetobacterium and Dehalobacter spp. was obtained, where Dehalobacter grew during dechlorination. The isolated Acetobacterium strain did not dechlorinate 1,2-DCA. Quantitative PCR with specific primers showed that Dehalobacter cells did not grow in the absence of a chlorinated electron acceptor and that the growth yield with 1,2-DCA was 6.9 (؎0.7) ؋ 10 7 16S rRNA gene copies/mol 1,2-DCA degraded. PCR with degenerate primers targeting reductive dehalogenase genes detected three distinct Dehalobacter/Desulfitobacterium-type sequences in the mixed-parent culture, but only one of these was present in the 1,2-DCA-H 2 coculture. Reverse transcriptase PCR revealed the transcription of this dehalogenase gene specifically during the dechlorination of 1,2-DCA. The 1,2-DCA-H 2 coculture could dechlorinate 1,2-DCA but not 1,1,2-TCA, nor could it dechlorinate chlorinated ethenes. As a collective, the genus Dehalobacter has been show to dechlorinate many diverse compounds, but individual species seem to each have a narrow substrate range.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene

Grostern¹,

Edwards

2009

Appl Environ Microbiol

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“…Acetobacterium sp. strain 69, isolated by Inoue et al . (1992) as a high producer of intracellular vitamin B 12 , was obtained from Tsukuba Research Center, Daicel Chemical Industries, Ltd (Tsukuba, Ibaragi, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The nutritional requirements of an acetogen recently isolated from sea sediments and classified as Acetobacterium sp. (Inoue et al 1992) has been studied using methanol-CO 2 (Bainotti et al 1998), but other unicarbon mixtures might be proposed (Lebloas et al 1994). In view of this, it may be reasonable to suppose that formate may be another possible carbon substrate for Acetobacterium sp.…”

Section: Introductionmentioning

confidence: 99%

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Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Bainotti

Nishio

2000

J Appl Microbiol

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A .E . B A IN OT T I A ND N . N IS H IO . 2000.The fermentative metabolism of Acetobacterium sp. grown on methanol-formate in continuous culture is described. The reaction stoichiometry of methanol-formate, including cells, were as follows: CH 3 OH ¦ 1·13HCOOH : 0·87CH 3 COOH ¦ 0·47 cell C. Formate enhanced growth yields by approximately 60% compared with methanol-CO 2 -grown cultures. Comparison of yields on methanol-formate allowed calculation of an energy yield of 1·3 mol ATP per mol acetate formed during homoacetate fermentation. The magnitudes of Y EG , the theoretical maximum yield of Y E , and m, the maintenance coefficient, were determined by growing the organism in methanol-formate and resulted in 16·5 g cell (mol methanol catabolized) −1 and 0·674 mmol methanol catabolized (g cell) −1 h −1 , respectively. It is concluded that formate might replace CO 2 as a source of carboxyl donor.

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“…It has been reported that various acetogenic anaerobic bacteria including Acetobacterium spp. and Sporomusa ovata produce large amounts of corrinoids 12,28) , and one of the acetateutilizing methanogenic archaea, Methanosarcina barkeri, produced vitamin B 12 -compound (Factor III) during cultivation in a methanol-minimum medium. The methanogenic strain excreted 80% of the B 12 compound produced into the medium without cell lysis 21) .…”

Section: Discussionmentioning

confidence: 99%

Effects of Plant Residue Extract and Cobalamin on Growth and Propionate Production of Propionicimonas paludicola Isolated from Plant Residue in Irrigated Rice Field Soil

Akasaka

Ueki

2004

Microb. Environ.

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Since most of the anaerobic bacterial isolates from rice plant residue in irrigated rice field soil grew slowly or weakly in the medium (PY medium) used, growth factors for the isolates were investigated. Plant residue extract (RE) was prepared by autoclaving plant residue collected from the soil, and RE was added to the medium as a possible source of growth factors. With the addition, growth of the slowly-growing, propionate-producing strains of Propionicimonas paludicola was considerably improved. Lactate was the dominant product of these strains in the presence of lower concentrations of RE. Moreover, amounts of acetate and propionate produced increased in proportion to the RE concentration added. The factor in RE affecting the growth of these strains appeared to be cobalamin, and addition of cobalamin to the medium remarkably improved their growth. With a sufficient amount of cobalamin added, propionate was the dominant product from the onset of fermentation, while lactate was only a minor product. Concentrations of cobalamin in RE were determined using the microbiological method. Almost exactly the same amount of cobalamin was detected in RE prepared from plant residue samples collected in different years. Cobalamin was also detected in extracts of the rice field soil, although the concentrations were much lower than those in RE. It was suggested that these cobalamin-requiring, propionateproducing bacteria survive in rice field soil by using cobalamin supplied by other microbes, which endogenously produce cobalamin and release it into the environment.

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Vitamin B2 production by Acetobacterium sp. and its tetrachloromethane-resistant mutants

Cited by 24 publications

References 12 publications

Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene

Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene

Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Effects of Plant Residue Extract and Cobalamin on Growth and Propionate Production of Propionicimonas paludicola Isolated from Plant Residue in Irrigated Rice Field Soil

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