Vitamin B-12-Deficiency Affects Immunoglobulin Production and Cytokine Levels in Mice

Funada, Ulala; Wada, Masahiro; Kawata, Toshitsugu; Mori, Kazumi; Tamai, Hiroko; Isshiki, Tomoko; Onoda, Junko; Tanaka, Nobuo; Tadokoro, Tadahiro; Maekawa, Akio

doi:10.1024/0300-9831.71.1.60

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…As described above, Wada et al [14] and Funada et al [15] reported Th2 polarization in vitamin B 12 -deficient animals. However, there was no significant difference in the percentages of Th1 and Th2 cells and the Th1/Th2 ratio between normal controls and PA patients in the present study.…”

Section: Discussionmentioning

confidence: 90%

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High Percentage of Regulatory T Cells before and after Vitamin B<sub>12</sub> Treatment in Patients with Pernicious Anemia

et al. 2014

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Introduction: In some previous studies, vitamin B₁₂ treatment showed immunomodulatory effects and restored the immunological abnormalities in patients with pernicious anemia (PA). In the present study, peripheral blood T cell subsets, including regulatory T cells (T_regs), were examined before and after vitamin B₁₂ treatment in PA patients. Patients and Methods: The percentages of CD4, CD8, Th1, Th2 and T_regs were examined in 23 PA patients before vitamin B₁₂ treatment, in 23 other PA patients after vitamin B₁₂ treatment and in 28 healthy controls. Results: The mean percentage of CD8+ T cells was significantly higher in the control group (23.0%; 95% CI, 20.4-25.6%) than in the pre- (16.0%; 95% CI, 12.1-20.0%) and posttreatment groups (15.2%; 95% CI, 11.8-18.6%; p < 0.05). The CD4/CD8 ratio was significantly lower in the control group (2.01; 95% CI, 1.66-2.34) than in the pre- (3.45; 95% CI, 2.55-7.80) and posttreatment groups (2.97; 95% CI, 2.22-3.72; p < 0.05). There was no significant difference in the mean Th1/Th2 ratio among these groups. There were significant increases in the mean percentage of T_regs in the pre- (6.29%; 95% CI, 5.04-7.54%) and posttreatment groups (7.77%; 95% CI, 6.34-9.20%) compared with the control group (4.18%; 95% CI, 3.92-4.47%; p < 0.05). Conclusions: The percentage of T_regs was significantly higher in PA patients than in normal subjects, and this high T_reg percentage was not different before and after vitamin B₁₂ treatment. Other immunological alterations also did not recover after vitamin B₁₂ treatment, so that these immunological changes appear to be the cause of PA and are not induced by vitamin B₁₂ deficiency.

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Section: Discussionmentioning

confidence: 90%

“…Funada et al [15] reported that Th2 polarization was observed in vitamin B 12 -deficient mice. These reports proposed that vitamin B 12 may have some immune-modulatory effects in animals and humans.…”

Section: Discussionmentioning

confidence: 99%

High Percentage of Regulatory T Cells before and after Vitamin B<sub>12</sub> Treatment in Patients with Pernicious Anemia

et al. 2014

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“…Vitamin A deficiency has also been shown to reduce the CD4 ϩ cell proportion in humans (39). A few in vivo studies showed a decrease in CD8 ϩ cell population with deficiencies in vitamin D (40), vitamin E (41), and vitamin B12 (42)(43)(44). Interestingly, vitamin B12 plays an important role in DNA metabolism and B12 deficiency can cause uracil misincorporation into DNA by the same mechanism as folate deficiency…”

Section: Discussionmentioning

confidence: 99%

Folate Deficiency Inhibits the Proliferation of Primary Human CD8+ T Lymphocytes In Vitro

Courtemanche

Elson-Schwab

Mashiyama

et al. 2004

The Journal of Immunology

148

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Folate is required for one-carbon transfer reactions and the formation of purines and pyrimidines for DNA and RNA synthesis. Deficiency of folate can lead to many clinical abnormalities, including macrocytic anemia, cardiovascular diseases, birth defects, and carcinogenesis. The nucleotide imbalance due to folate deficiency causes cell cycle arrest in the S phase and uracil misincorporation into DNA, which may result in DNA double-strand breaks during repair. The role of folate in the immune system has not been fully characterized. We cultured PHA-activated human T lymphocytes in varying concentrations of folate, and measured proliferation, cell cycle, apoptosis, uracil misincorporation, and proportions of Th cells (CD4+) and cytotoxic T (CD8+) cells. Folate deficiency reduced proliferation of T lymphocytes, induced cell cycle arrest in the S phase, induced apoptosis, and increased the level of uracil in DNA. Folate deficiency also increased the CD4+ to CD8+ ratio due to a marked reduction of CD8+ cell proliferation. Folate or nucleoside repletion of folate-deficient cells rapidly restored T lymphocyte proliferation and normal cell cycle, reduced the DNA uracil content, and lowered the CD4+ to CD8+ ratio. These data suggest that folate status may affect the immune system by reducing the capacity of CD8+ cells to proliferate in response to activation.

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“…C3, IgG, and IgM levels were low in mice with vitamin B12 deficiency [20]. Avci et al [14] reported a patient with IgG and IgM deficiency that recovered following vitamin B12 treatment.…”

Section: Discussionmentioning

confidence: 99%

Involuntary movements during vitamin B12 treatment in infants

Tosun¹,

Yz²,

Çeçen³

et al. 2011

Turk J Hematol

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Megaloblastic anemia is rare in infants and is generally due to vitamin B12 (cobalamin) deficiency in the mother. Neurologic symptoms of vitamin B12 deficiency include irritability, failure to thrive, hypotonia, and developmental regression/delay. Herein we present 2 infants with vitamin B12 that developed movement disorder 5 d after initiation of vitamin B12 treatment. Symptoms included tremor and myoclonus, involving in particular the face, tongue, and hands. Clinical findings in infants associated with vitamin B12 deficiency vary, and temporary involuntary movement can be observed during vitamin B12 therapy. (Turk J Hematol 2011; 28: 317-22) Key words: Involuntary movement, vitamin B12 deficiency, infant Received: June 11, 2010 Accepted: October 19, 2010 Özet Süt çocuklarında megaloblastik anemi nadirdir ve genellikle annelerdeki vitamin B12 eksikliğinin bir sonucudur. Kobalamin eksikliğinin nörolojik semptomları huzursuzluk, büyüme geriliği, hipotoni ve nöro-gelişimsel becerilerde gerileme/gecikmeyi içerir. Bu makalede kobalamin tedavisinin 5. gününde özellikle dil, yüz, elleri içine alan tremor ve miyoklonus gibi istemsiz hareketler gelişen, kobalamin eksikliği olan, iki süt çocuğu sunuldu. Süt çocuklarında kobalamin eksikliği farklı klinik bulgularla karşımıza gelebilir ve tedavisi sırasında geçici istemsiz hareketler görülebilir. (Turk J Hematol 2011; 28: 317-22)

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Vitamin B-12-Deficiency Affects Immunoglobulin Production and Cytokine Levels in Mice

Cited by 14 publications

References 26 publications

High Percentage of Regulatory T Cells before and after Vitamin B<sub>12</sub> Treatment in Patients with Pernicious Anemia

High Percentage of Regulatory T Cells before and after Vitamin B<sub>12</sub> Treatment in Patients with Pernicious Anemia

Folate Deficiency Inhibits the Proliferation of Primary Human CD8+ T Lymphocytes In Vitro

Involuntary movements during vitamin B12 treatment in infants

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