Vital staining of<i>Histoplasma capsulatum</i>with Janus Green B

“…with this dose. Viability of the fungic suspensions, determined using Janus Green B vital dye [ 11 ], was always higher than 80%. Saline was injected as controls.…”

Section: Infection Of Micementioning

confidence: 99%

Alterations in the pathogenicity of oneParacoccidioides brasiliensis isolate do not correlative with itsin vitro growth

et al. 1990

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The in vitro subcultivation of some microorganisms for long periods causes measurable loss of their pathogenicity, which can be reverted by reisolation from infected hosts. We compared the pathogenicity and the in vitro growth pattern of one P. brasiliensis isolate (Pb 18) in its yeast phase, using the following samples: 1) The original pathogenic Pb 18 (OP). 2) Pb 18 attenuated by continuous in vitro subcultivation (AT). 3) Pb 18 (AT) reisolated from susceptible B10.A mice (RS). 4) Pb 18 (AT) reisolated from resistant A/SN mice (RR). Pathogenicity was evaluated by anatomopathology and mortality of mice infected i.p. with 5 x 10(6) fungi. Median survival times of mice infected with OP ranged from 74 to 117 days during the first 51 months of subculturing; with more cycles of subculturing the median survival time increased, reaching 250 days at the 64th month. This indicated decreasing virulence of OP during this period of subculturing. Survival of mice infected with RS and RR was respectively 112 and 123 days, which is similar to the behavior of the OP variant. The in vitro growth curve profile of RR showed significantly higher numbers of total and viable yeasts than the other studied variant. These results show that: 1) Pb 18 isolate loses its pathogenicity by continuous subcultivation. This phenomenon is reverted by reisolation from mice, independently from their susceptibility to the fungus; 2) the in vitro growth patterns of Pb 18 do not correlate with alterations in pathogenicity but are influenced by the host's environment.

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“…To prepare the inoculum, the cells were washed three times with sterile phosphate buffer solution (PBS) pH 7.2. The cellular viability was determined using the Janus Green vital stain (Berliner and Reca, 1966). Cultures with a minimum of 80% viability were used and the concentration adjusted by counting in a Neubauer chamber.…”

Section: Fungusmentioning

confidence: 99%

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

Takahachi

Maluf

Svidzinski

et al. 2006

Braz. arch. biol. technol.

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Vital staining ofHistoplasma capsulatumwith Janus Green B

Cited by 74 publications

References 2 publications

Caracterização da função das células TCD4+ e TCD8+ na Paracoccidioidomicose pulmonar de camundongos isogênicos

Caracterização da função das células TCD4+ e TCD8+ na Paracoccidioidomicose pulmonar de camundongos isogênicos

Alterations in the pathogenicity of oneParacoccidioides brasiliensis isolate do not correlative with itsin vitro growth

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

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