Visualizing Intercellular Tensile Forces by DNA-Based Membrane Molecular Probes

Zhao, Bin; O’Brien, Casey P.; Mudiyanselage, Aruni P. K. K. Karunanayake; Li, Ningwei; Bagheri, Yousef; Wu, Rigumula; Sun, Yubing; Meng, Yuchuan

doi:10.1021/jacs.7b11176

Cited by 73 publications

(90 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EC-DNAMeter was prepared using a Protein G linker to couple the IgG/Fc-fused E-cadherin with the DNAMeter (Methods). Compared with direct chemical conjugation, the Protein G linker helps to avoid the loss of E-cadherin activities 28,40 . In addition, to allow the probe to insert onto MDCK cell membranes, a cholesterol anchor was conjugated at the other end of the DNAMeter.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Quantifying Tensile Forces at Cell–Cell Junctions with a DNA-based Fluorescent Probe

Zhao

Xie

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Cells are physically contacting with each other. Direct and precise quantification of forces at cell-cell junctions is still challenging. Herein, we have developed a DNA-based ratiometric fluorescent probe, termed DNAMeter, to quantify intercellular tensile forces. These lipidmodified DNAMeters can spontaneously anchor onto live cell membranes. The DNAMeter consists of two self-assembled DNA hairpins of different force tolerance. Once the intercellular tension exceeds the force tolerance to unfold a DNA hairpin, a specific fluorescence signal will be activated, which enables the real-time imaging and quantification of tensile forces. Using Ecadherin-modified DNAMeter as an example, we have demonstrated an approach to quantify, at the molecular level, the magnitude and distribution of E-cadherin tension among epithelial cells.Compatible with readily accessible fluorescence microscopes, these easy-to-use DNA tension probes can be broadly used to quantify mechanotransduction in collective cell behaviors.

show abstract

Section: Resultsmentioning

confidence: 99%

“…We have recently developed a DNA-based probe to visualize intercellular tensile forces at cell-cell junctions 28 . In this system, a pair of cholesterol anchors was used to insert this DNA hairpin-based probe onto live cell membranes 29,30 .…”

Section: Introductionmentioning

confidence: 99%

Quantifying Tensile Forces at Cell–Cell Junctions with a DNA-based Fluorescent Probe

Zhao

Xie

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fluorescence imaging showing clustering and probe opening at the intracellular junctions as indicated by the increase in junction/membrane fluorescence ratio (Right). Reproduced with permission . Copyright 2017, American Chemical Society.…”

Section: Dna‐based Force Probes To Map Piconewton Forces Within Cellsmentioning

confidence: 99%

“…An interesting development in the area of membrane‐tethered DNA probes is a class of probes that physically bridges two cells. To map mechanical forces transmitted at the junction between two living cells, You and co‐workers modified the anchoring terminus of the DNA hairpin tension probe with a pair of cholesterol moieties, so that the probes can be spontaneously assembled on a cell surface with high kinetic stability (Figure C) . This design was used to investigate intracellular tension of E‐cadherin and integrin receptors.…”

Section: Dna‐based Force Probes To Map Piconewton Forces Within Cellsmentioning

confidence: 99%

DNA Nanotechnology as an Emerging Tool to Study Mechanotransduction in Living Systems

Pui‐Yan

Salaita

2019

Small

View full text Add to dashboard Cite

The ease of tailoring DNA nanostructures with sub‐nanometer precision has enabled new and exciting in vivo applications in the areas of chemical sensing, imaging, and gene regulation. A new emerging paradigm in the field is that DNA nanostructures can be engineered to study molecular mechanics. This new development has transformed the repertoire of capabilities enabled by DNA to include detection of molecular forces in living cells and elucidating the fundamental mechanisms of mechanotransduction. This Review first describes fundamental aspects of force‐induced melting of DNA hairpins and duplexes. This is then followed by a survey of the currently available force sensing DNA probes and different fluorescence‐based force readout modes. Throughout the Review, applications of these probes in studying immune receptor signaling, including the T cell receptor and B cell receptor, as well as Notch and integrin signaling, are discussed.

show abstract

“…DNA is a versatile, programmable scaffold that is highly suited to chemically imaging living systems (23)(24)(25)(26)(27). A range of detection chemistries displayed on DNA nanodevices have been used to quantitatively map diverse analytes in living systems (26)(27)(28)(29). By 25 leveraging the precise stoichiometry of DNA hybridization, one can incorporate a reference fluorophore and the desired detection chemistry in a well-defined stoichiometry to yield ratiometric probes (30).…”

Section: Main Textmentioning

confidence: 99%