Visualization of yeast single-cells on fabric surface with a fluorescent glucose and their isolation for culture

Abstract

An ultra-deep focusing range (UDF) fluorescent microscope system has been combined with a micromanipulation system to develop a viable cell detection-identification system applicable to microbes on environmental surfaces and products. Candida albicans yeast cells on a fabric sample surface were viably stained with a fluorescent glucose derivative, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy glucose (2-NBDG) and detected with a UDF fluorescent microscope. Visualized single-cells of C. albicans were picked in a glass microcapillary and transferred onto an agar medium. After the culture, the colony was assayed for DNA sequence to identify the isolate. This demonstrates a potential application to the study of unknown environmental microorganisms.

Research On Lqr Zone Control Algorithm For Picking Robot Arm

“…Its method is that we can decompose the acceleration in the Cartesian coordinates into the corresponding joint acceleration, then we can solve the control moment which apply to the joint motor according to the corresponding system dynamics model. By solving the derivative of equation 6, we can gain the picking robot arm acceleration ( ) ( ) ( ) ( ) X t J t q t Jq t  (10) Equation 10represents the corresponding acceleration relations in the Cartesian coordinates and the joint coordinate system, namely the decomposition control of the desired acceleration in the Cartesian coordinates. The target of acceleration control is virtually to make the precision error between the trajectory and the desired position evaluation converge to zero, now assuming that d e X X  (11) Where, e denotes the precision error between the actual position and the desired evaluation position, d X denotes the desired position vector , X denotes the actual position vector.…”

“…The value of the ratio coefficient should make the characteristic root of the real part be negative. Substituting the equation (10) and equation (11) into the equation 12, we can gain…”

“…Research literature [9][10] represented that the dynamic equation based on Lagrange theory is applied to the study of arm dynamic model, its derivation process is simple, and it can clearly represent the coupling characteristic of arms, and can also directly represent the system control input function. Therefore we can establish the dynamics equation by using the recursive Lagrange equation in the form of homogeneous coordinates.…”

Rapid and retrievable recording of big data of time-lapse 3D shadow images of microbial colonies

“…To date various rapid methods have been developed using non-culture or micro-colony culture systems 2 3 4 5 6 7 8 . Staining of viable cells with a specific fluorescent dye, for example, such as fluorescein diacetate derivatives 9 10 11 and fluorescent glucose 12 13 can be used to determine the number of viable cells rapidly within half an hour, requiring no time for growth verification. On the other hand, micro-colony count is based on culture on an agar plate and is able to provide count result relatively fast by using a microscope imaging system 14 .…”