Rapid and retrievable recording of big data of time-lapse 3D shadow images of microbial colonies

Abstract

We formerly developed an automatic colony count system based on the time-lapse shadow image analysis (TSIA). Here this system has been upgraded and applied to practical rapid decision. A microbial sample was spread on/in an agar plate with 90 mm in diameter as homogeneously as possible. We could obtain the results with several strains that most of colonies appeared within a limited time span. Consequently the number of colonies reached a steady level (Nstdy) and then unchanged until the end of long culture time to give the confirmed value (Nconf). The equivalence of Nstdy and Nconf as well as the difference of times for Nstdy and Nconf determinations were statistically significant at p < 0.001. Nstdy meets the requirement of practical routines treating a large number of plates. The difference of Nstdy and Nconf, if any, may be elucidated by means of retrievable big data. Therefore Nconf is valid for official documentation.

Case study on the environmental monitoring for biological manufacturing using Time-lapse Shadow Image Analysis

“…The alternative method for the EM application in the manufacturing facilities requires that the accuracy, consistency, standardization of procedure, convenience, and practical technology accumulation in addition to rapidity are standardized. Among these methods, we focused on the MicroBio µ3D AutoScanner system based on Time-lapse Shadow Image Analysis (TSIA) [24,25], to detect the colony directly and rapidly on the agar plate in this study. The correlation of colony count between both TSIA and VCC for E.coli were already reported [25].…”

Detection Time Distribution of Microcolonies Formed by Individual Heat-Injured Cells of <i>Escherichia coli</i>

“…A rapid method to detect microcolonies formed on an agar plate has also been developed (London et al, 2010;Ogawa et al, 2012) . In this method, an apparatus named MicroBio µ3D TM AutoScanner (Nippon Becton Dickinson Co., Ltd., Tokyo) equipped with the time-lapse shadow image analysis system is used (Ogawa et al, 2015) . The injured cells have mostly been detected and enumerated by using the double agar plating method, using both non-selective and selective media, the latter of which contains a growth-inhibiting substance selective to injured cells, e.g., sodium chloride or sodium deoxycholate.…”