Visualization of porcine eye anatomy by X-ray microtomography

Leszczyński, Bartosz; Sojka-Leszczyńska, Paulina; Wojtysiak, Dorota; Wróbel, Andrzej; Pędrys, R.

doi:10.1016/j.exer.2017.11.004

Cited by 12 publications

(36 citation statements)

References 10 publications

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“…We used Lugol staining solution to enhance the X-ray attenuation within the samples ( 48 ). The samples were fixed with 4% formaldehyde and placed in an individual dish containing 50 mL Lugol solution (I3K) (Cat.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Developing a Novel Positronium Biomarker for Cardiac Myxoma Imaging

Moskal

Kubicz

Grudzień

et al. 2021

Preprint

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Here, positronium imaging is presented to determine cardiac myxoma (CM) extracted from patients undergoing urgent cardiac surgery due to unexpected atrial masses. Positronium is an atom build from an electron and a positron, produced copiously in intra-molecular voids during the PET imaging. CM, the most common cardiac tumor in adults, accounts for 50-75% of benign cardiac tumors. We aimed to assess if positronium serves as a biomarker for diagnosing CM. Perioperative examinations and histopathology staining in six patients confirmed the primary diagnosis of CM. We observed significant differences in the mean positronium lifetime between tumor and normal tissues, with an average value of 1.92(02) ns and 2.72(05) ns for CM and the adipose tissue, respectively. Our findings, combined with positronium lifetime imaging, reveals the novel emerging positronium biomarker for cardiovascular imaging.One-Sentence SummaryPositronium may serve as an imaging biomarker for cancer diagnostics.

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Section: Supplementary Materialsmentioning

confidence: 99%

Developing a Novel Positronium Biomarker for Cardiac Myxoma Imaging

Moskal

Kubicz

Grudzień

et al. 2021

Preprint

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“…PTA is known for its low penetration rate (16) and was demonstrated here particularly on the lens. The 30 weeks applied in our study exceeded by far the incubation times on previous approaches [96 h, see (14)]. For the visualization of the lens, iodine based contrast ag• ents are more suitable than PTA for which the incubation times of more than 30 weeks applied here was still insufficient.…”

Section: Discussionmentioning

confidence: 78%

“…The applied contrast agents have been used primarily because of their relative ease of use and low toxicity ( 16 , 18 ) while osmium has been used successfully for contrasting eyes in previous studies ( 12 , 14 ). In particular, iodine-containing solutions have proven successful for contrasting ocular tissue ( 13 - 15 ) and was confirmed with this study.…”

Section: Discussionmentioning

confidence: 99%

“…Micro-computed tomography (micro-CT) is the imaging method of choice when it comes to combine high-resolution imaging with 3D visualization in an adequate amount of time. As a consequence, micro-CT based imaging also finds its way more and more to ophthalmological anatomy (12)(13)(14)(15). Nonetheless, various challenges and obstacles accompany micro-CT based imaging.…”

Section: Introductionmentioning

confidence: 99%

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Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Runge¹,

Stahnke²,

Guthoff³

et al. 2022

Quant Imaging Med Surg

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Background: Micro-computed tomography (micro-CT) provides detailed 3-dimensional (3D) visualization of anatomical structures and encourages morphological reinvestigation of organs with delicate features. The low radiodensity of soft tissues necessitates preceding sample preparation to conduct X-ray imaging with decent contrast between different tissues. In this study, we demonstrate the preparation with three radiopaque agents in combination with elimination of liquids by critical point drying (CPD) introduced for ocular samples.Methods: Enucleated porcine eyes were prepared with ethanolic iodine (EI), aqueous iodine-potassium iodide, or ethanolic phosphotungstic acid (EPTA). Micro-CT scans of the samples were conducted in a moist environment with an isotropic resolution of 9.2-12.5 µm voxel size. Subsequently, samples were chemically dehydrated and critical point (CP) dried to conduct a second scan in a dry environment with a resolution up to 4.7-5.4 µm in voxel size. The visualization effects were qualitatively and semi-quantitatively evaluated with regard to the generated contrast between different ocular tissues.Results: All three contrast agents accumulated well in most of the investigated ocular tissues and lead to an increased X-ray attenuation which allowed for differentiated visualization of ocular structures. Problematic agent penetration into the lens was obvious for iodine-potassium iodide and EPTA. Artificial damages of the lens and thickness reduction for the cornea and sclera due to CPD were noticed. The effects of the different contrasting treatments are described and compared with regard to the effects of CPD. Exclusively CP dried samples that were not treated with contrast agents could also be visualized excellently with a good distinction of different ocular structures from each other.Conclusions: All ocular structures can be visualized by micro-CT. To contrast moist samples, the best results were achieved with iodine potassium iodide (IPI). CPD improved the scan quality in all cases. Even without pretreatment with contrasting agents, the CP dried samples showed a contrast similar to the IPI treated samples.

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“…Micro-CT, together with iodine staining, provides excellent results for investigation of BHM in a hamster eye. This is the only method that enables both qualitative and quantitative analyses of the globe at a histological level in 3D [ 20 ]. Visualization of tumor vascularization against the background of a normal globe anatomy provides a unique opportunity to study the spatial relationship of BHM.…”

Section: Discussionmentioning

confidence: 99%

Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye

Leszczyński

Śniegocka

Wróbel

et al. 2018

IJMS

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A tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensional (3D) structure only provides partial information. Therefore, we propose the use of microcomputed tomography (micro-CT) imaging to analyze the evolution of a tumor vasculature in an experimental ocular tumor model. A Bomirski Hamster Melanoma was implanted in the anterior chamber of a hamster eye. Ultrasound (US) imaging of the same tumor was performed in vivo, and the vascular results obtained using the two methods were compared. Normal ocular tissues, a tumor, and a tumor vascular structure were revealed with high accuracy using micro-CT. The vessels that grew within the tumor were chaotic, leaky, and contained many convoluted micro-vessels and embolizations. They comprised 20–38% of the tumor mass. The blood flow in the larger functional vessels was in the range from 10 to 25 mm/s, as determined by in vivo Doppler US. The micro-CT imaging of the hamster eyeball enabled both qualitative and quantitative 3D analyses of the globe at a histological level. Although the presented images were obtained ex vivo, micro-CT noninvasive imaging is being developed intensively, and high-resolution in vivo imaging is feasible.

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Visualization of porcine eye anatomy by X-ray microtomography

Cited by 12 publications

References 10 publications

Developing a Novel Positronium Biomarker for Cardiac Myxoma Imaging

Developing a Novel Positronium Biomarker for Cardiac Myxoma Imaging

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye

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