Visualization of materials using the confocal laser scanning microscopy technique

Teng, Xu; Li, Feng; Lu, Chao

doi:10.1039/c8cs00061a

Cited by 52 publications

(37 citation statements)

References 192 publications

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“…Air objective and oil-immersion objectives are widely used in scanning microscopy, such as scanning confocal and stimulated emission depletion (STED) microscopy, which are fundamental instruments in biological, biomedical and material researches. 21,22 In the imaging process, the interface with refractive indices discontinuous is inevitably inclined or deformed during the imaging process. In the microscopes equipped with an air objective lens, the oblique angle of inclined interface can be even large due to the long working distance (WD).…”

Section: Resultsmentioning

confidence: 99%

“…Air objective (such as RMS40X‐PF and N60X‐PF from Nikon) has been widely used in confocal microscope for scanning surface structures of materials, 21 where the materials are placed on glass slides. In case 1, a 40× NA 0.75 objective lens is studied.…”

Section: Resultsmentioning

confidence: 99%

“…Oil-immersion objective (RMS100X-PFO, Olympus) lens is also widely used in confocal microscope for scanning bio-logical samples. 22 Here, a typical 100X NA 1.3 objective lens is studied as an example. 1 and 2 respectively refer to the refractive indices of glass and biological samples, and assigned to 1.52 and 1.33 respectively.…”

Section: Influence Of Oblique Interface With Objective Lensmentioning

confidence: 99%

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Aberration on excitation focal spot caused by oblique interface with refractive indices discontinuous and its correction with pure‐phase compensation for laser scanning microscopy

Zhu

Zhang

Zhao

et al. 2021

Journal of Microscopy

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The interface of mediums with refractive indices discontinuous, for example airglass and glass-water, are inevitable in microscopic imaging. In this work, the aberration of oblique interface with refractive index discontinuous on the laser scanning microscope was investigated theoretically with numerical simulations. It was found that the position, shape and FWHM of focal spots, were all significantly affected by the aberration due to oblique interface. The aberration can cause serious shifting of focal spots in the axial direction of beam during -scanning and lead to an inaccurate reconstruction of three-dimensional (3D)targets. The aberration can also lead to a decreasing spatial resolution. To correct the influence of the aberration, a pure-phase modulation method has been proposed. By applying a phase compensation map into a spatial light modulator (SLM), the oblique interface aberration had been corrected experimentally in a laser scanning microscope. We hope this research can attract the attention of researchers when using scanning microscope, especially for reconstructing 3D biological and material structures.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Influence Of Oblique Interface With Objective Lensmentioning

confidence: 99%

See 1 more Smart Citation

Aberration on excitation focal spot caused by oblique interface with refractive indices discontinuous and its correction with pure‐phase compensation for laser scanning microscopy

Zhu

Zhang

Zhao

et al. 2021

Journal of Microscopy

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“…TEM analysis revealed the presence of spherical particles of oil phase with a dimension under 100 nm, stabilized in the aqueous medium [190]. In the same direction, confocal laser microscopy can be a useful tool with application in material structure analysis, being largely accessed for microemulsion characterization [191].…”

mentioning

confidence: 96%

“…In the same direction, confocal laser microscopy can be a useful tool with application in material structure analysis, being largely accessed for microemulsion characterization [191].…”

mentioning

confidence: 99%

Foray into Concepts of Design and Evaluation of Microemulsions as a Modern Approach for Topical Applications in Acne Pathology

et al. 2020

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With a fascinating complexity, governed by multiple physiological processes, the skin is considered a mantle with protective functions which during lifetime are frequently impaired, triggering dermatologic disorders. As one of the most prevalent dermatologic conditions worldwide, characterized by a complex pathogenesis and a high recurrence, acne can affect the patient’s quality of life. Smart topical vehicles represent a good option in the treatment of a versatile skin condition. By surpassing the stratum corneum known for diffusional resistance, a superior topical bioavailability can be obtained at the affected place. In this direction, the literature study presents microemulsions as a part of a condensed group of modern formulations. Microemulsions are appreciated for their superior profile in matters of drug delivery, especially for challenging substances with hydrophilic or lipophilic structures. Formulated as transparent and thermodynamically stable systems, using simplified methods of preparation, microemulsions have a simple and clear appearance. Their unique structures can be explained as a function of the formulation parameters which were found to be the mainstay of a targeted therapy.

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In Operando Visualization and Dynamic Manipulation of Electrochemical Processes at the Electrode–Solution Interface

et al. 2022

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Revealing the dynamic processes at the electrode–solution interface is imperative for understanding electrochemical phenomena. Most techniques have been developed to sense the electrode surface changes at the nanoscale, but provide limited information on potential‐induced interfacial ion redistribution at the mesoscale. Herein, we present an in operando visualization method utilizing a microfabricated electrochemical cell combined with a laser scanning confocal microscope to observe high‐resolution and fast‐response interfacial processes. We report potential‐induced formation and transformation of the Nernst diffusion layer, demonstrating that pulsed voltage dynamically perturbs the interface and promotes ion diffusion. This provides an additional insight into developing a dynamic manipulation method to control the electrochemical process. Our novel visualization method can easily be applied to monitor different ionic behaviors in electrochemical reactions at the mesoscale.

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Visualization of materials using the confocal laser scanning microscopy technique

Abstract: This review summarizes the recent applications of confocal laser scanning microscopy in materials science.

Cited by 52 publications

References 192 publications

Aberration on excitation focal spot caused by oblique interface with refractive indices discontinuous and its correction with pure‐phase compensation for laser scanning microscopy

Aberration on excitation focal spot caused by oblique interface with refractive indices discontinuous and its correction with pure‐phase compensation for laser scanning microscopy

Foray into Concepts of Design and Evaluation of Microemulsions as a Modern Approach for Topical Applications in Acne Pathology

In Operando Visualization and Dynamic Manipulation of Electrochemical Processes at the Electrode–Solution Interface

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