Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging

Ткачев, С И; Mitrin, B. I.; Karnaukhov, Nikolay S.; Sadyrin, Evgeniy V.; Voloshin, Mark; Maksimov, Alexey; Goncharova, Anna S.; Lukbanova, Ekaterina A.; Zaikina, Ekaterina V.; Volkova, Anastasiya V.; Khodakova, D. V.; Mindar, Maria V.; Yengibarian, Marina A.; Protasova, Tatyana P.; Kit, Sergey O.; Ermakov, Alexey; Chapek, Sergei V.; Tkacheva, Marina

doi:10.1016/j.exer.2020.108394

Cited by 10 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At least we could be able to differentiate types of tissues according to MGV at transversal slides of 3D reconstructions of corpora cavernosa, as shown in the study by Tkachev et al 42 Also, we suggest that nucleus-specific staining prior to micro-CT could enable appropriate 3D visualization of smooth muscle cells replacement in CF. 43 For direct penile density assessment, calibration phantoms with set density are needed.…”

Section: Discussionsupporting

confidence: 57%

“…We believe that larger magnification and smaller voxel size can better visualize tissue remodeling during CF. At least we could be able to differentiate types of tissues according to MGV at transversal slides of 3D reconstructions of corpora cavernosa, as shown in the study by Tkachev et al 42 . Also, we suggest that nucleus‐specific staining prior to micro‐CT could enable appropriate 3D visualization of smooth muscle cells replacement in CF 43 .…”

Section: Discussionsupporting

confidence: 57%

“…For example, Tkachev et al. made a 3D reconstruction of the human's eye part that allowed differing superficial and basal epithelium of the cornea 42 . In this study, the magnification objective was 4x with voxel size 2.5 μm.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Tkachev et al made a 3D reconstruction of the human's eye part that allowed differing superficial and basal epithelium of the cornea. 42 In this study, the magnification objective was 4x with voxel size 2.5 μm. While in our work, acquisition parameters for corpora cavernosa were 0.4x for magnification objective and 50 μm for voxel size, resulting in relatively less detailed visualization.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective:The aim was to evaluate micro-computed tomography for assessment of cavernous fibrosis and compare it with histological, histochemical, immunohistochemical, and RT-PCR analysis. Materials and methods:A controlled trial was performed involving 25 New Zealand male rabbits with induced testosterone deficiency by orchidectomy. Penile samples were obtained before and after 7, 14, 21, and 84 days from orchidectomy. We consistently performed (a) gray value analysis of corpora cavernosa 3D models reconstructed after micro-computed tomography, (b) morphometry of smooth muscles/connective tissue ratio, collagen type I/III ratio, and area of TGF-beta-1 expression in corpora cavernosa, and (c) RT-PCR of TGF-beta-1 expression.Results: Micro-computed tomography allowed visualization of penile structures at a resolution comparable to light microscopy. Gray values of corpora cavernosa decreased from 1673 (1512-1773) on the initial day to 1184 (1089-1232) on the 21st day (p < 0.005). However, on the 84th day, it increased to 1610 (1551-1768). On 21st and 84th days, there was observed a significant decrease in smooth muscle/connective tissue ratio and a significant increase in collagen type I/III ratio (p < 0.05). TGF-beta1 expression increased on the 84th day according to immunohistochemistry (p < 0.005).RT-PCR was impossible to conduct due to the absence of RNA in obtained samples after micro-CT.Discussion and conclusions: Micro-computed tomography provided 3D visualization of entire corpora cavernosa and assessment of radiodensity alterations by gray value

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Micro-computed tomography (micro-CT) is the imaging method of choice when it comes to combine high-resolution imaging with 3D visualization in an adequate amount of time. As a consequence, micro-CT based imaging also finds its way more and more to ophthalmological anatomy (12)(13)(14)(15). Nonetheless, various challenges and obstacles accompany micro-CT based imaging.…”

Section: Introductionmentioning

confidence: 99%

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Runge¹,

Stahnke²,

Guthoff³

et al. 2022

Quant Imaging Med Surg

View full text Add to dashboard Cite

Background: Micro-computed tomography (micro-CT) provides detailed 3-dimensional (3D) visualization of anatomical structures and encourages morphological reinvestigation of organs with delicate features. The low radiodensity of soft tissues necessitates preceding sample preparation to conduct X-ray imaging with decent contrast between different tissues. In this study, we demonstrate the preparation with three radiopaque agents in combination with elimination of liquids by critical point drying (CPD) introduced for ocular samples.Methods: Enucleated porcine eyes were prepared with ethanolic iodine (EI), aqueous iodine-potassium iodide, or ethanolic phosphotungstic acid (EPTA). Micro-CT scans of the samples were conducted in a moist environment with an isotropic resolution of 9.2-12.5 µm voxel size. Subsequently, samples were chemically dehydrated and critical point (CP) dried to conduct a second scan in a dry environment with a resolution up to 4.7-5.4 µm in voxel size. The visualization effects were qualitatively and semi-quantitatively evaluated with regard to the generated contrast between different ocular tissues.Results: All three contrast agents accumulated well in most of the investigated ocular tissues and lead to an increased X-ray attenuation which allowed for differentiated visualization of ocular structures. Problematic agent penetration into the lens was obvious for iodine-potassium iodide and EPTA. Artificial damages of the lens and thickness reduction for the cornea and sclera due to CPD were noticed. The effects of the different contrasting treatments are described and compared with regard to the effects of CPD. Exclusively CP dried samples that were not treated with contrast agents could also be visualized excellently with a good distinction of different ocular structures from each other.Conclusions: All ocular structures can be visualized by micro-CT. To contrast moist samples, the best results were achieved with iodine potassium iodide (IPI). CPD improved the scan quality in all cases. Even without pretreatment with contrasting agents, the CP dried samples showed a contrast similar to the IPI treated samples.

show abstract

Correlating the Mechanical Properties to the Mineral Density of Brown Spot Lesion in Dentine Using Nanoindentation and X-ray Micro-tomography

Sadyrin

2021

Advanced Structured Materials

View full text Add to dashboard Cite

Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging

Cited by 10 publications

References 38 publications

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Correlating the Mechanical Properties to the Mineral Density of Brown Spot Lesion in Dentine Using Nanoindentation and X-ray Micro-tomography

Contact Info

Product

Resources

About