Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy

Shaiu, Wen‐Ling; Vesenka, James; Jondle, Daniel; Henderson, Eric; Larson, Drena D.

doi:10.1116/1.578311

Cited by 21 publications

(23 citation statements)

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“…Previously, biotin-streptavidin-gold has been used to label linear DNA for imaging by AFM 25,26 . We have used 1.4-nm nanogold-streptavidin conjugates to label DAE molecules.…”

Section: Control Of Surface Topographymentioning

confidence: 99%

Design and self-assembly of two-dimensional DNA crystals

Winfree

Liu

Wenzler

et al. 1998

Nature

2,679

2,071

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Molecular self-assembly presents a 'bottom-up' approach to the fabrication of objects specified with nanometre precision. DNA molecular structures and intermolecular interactions are particularly amenable to the design and synthesis of complex molecular objects. We report the design and observation of two-dimensional crystalline forms of DNA that self-assemble from synthetic DNA double-crossover molecules. Intermolecular interactions between the structural units are programmed by the design of 'sticky ends' that associate according to Watson-Crick complementarity, enabling us to create specific periodic patterns on the nanometre scale. The patterned crystals have been visualized by atomic force microscopy.

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“…Previously, biotin-streptavidin-gold has been used to label linear DNA for imaging by AFM 25,26 . We have used 1.4-nm nanogold-streptavidin conjugates to label DAE molecules.…”

Section: Control Of Surface Topographymentioning

confidence: 99%

Design and self-assembly of two-dimensional DNA crystals

Winfree

Liu

Wenzler

et al. 1998

Nature

2,679

2,071

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“…17 A number of groups have utilized the high resolution (∼1 Å) of the AFM for examination of 2D crystallization of protein molecules. 11,[18][19][20][21] Others, especially Hansma, Bustamante, and Henderson, have studied isolated proteins 17,[22][23][24][25][26][27][28] or DNA molecules 12,[29][30][31][32][33] and their complexes. 34 Recently, AFM has been used by the Marchant group to obtain valuable structural information for the multimeric Von Willebrand factor (MW ∼ 260 kD, monomer).…”

Section: Introductionmentioning

confidence: 99%

Imaging the Molecular Dimensions and Oligomerization of Proteins at Liquid/Solid Interfaces

et al. 1998

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Individual Concanavalin A (ConA) molecules have been imaged at the liquid/solid interface with an atomic force microscope (AFM). Three-dimensional sizing with very high resolution (<5 Å) has been obtained by a novel approach based on height distributions, which avoids the tip convolution effects which normally affect scanning probe microscopy techniques. Each height measurement correlates to a particular molecular orientation on the surface. A large number of such measurements provide a statistical ensemble of orientations. The complete height distribution reflects the three-dimensional size of the protein sample and hence its tertiary and quaternary structure. A surface adsorption and orientation model, based on a minimization of surface adsorption energy, is proposed. This model is in good agreement with the observed height distribution of Con A molecules at the liquid/solid interface. Analysis of Con A and succinylated Con A molecules on mica demonstrates that Con A dimers are the prevalent species at the liquid/solid interface. This is in contrast to the tetrameric organization of Con A normally observed in solution. The new possibilities opened by height distribution analysis on the physical characterization of biomolecules at interfaces are also discussed.

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] The DNA molecule should be imaged under a near native condition. 1 A special effort has been dedicated to the visualization of deoxyribonucleic acid ͑DNA͒ molecules.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic atomization of the DNA solution for atomic force microscopy

Sasaki

Okuma

Miyata

et al. 1995

Journal of Vacuum Science &Amp; Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena

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Articles you may be interested inConductivity of DNA probed by conducting-atomic force microscopy: Effects of contact electrode, DNA structure, and surface interactionsWe have investigated the ultrasonic atomization of the deoxyribonucleic acid ͑DNA͒ solution as a simple preparation technique to image circular DNA molecules with the atomic force microscope. The DNA solution with a relatively high concentration ͑ϳ25 g/ml͒ is ultrasonically atomized at the exciting frequency of 2.4 MHz. Preliminary results show that this technique allows an even distribution of unaggregated strands.

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Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy

Cited by 21 publications

References 0 publications

Design and self-assembly of two-dimensional DNA crystals

Design and self-assembly of two-dimensional DNA crystals

Imaging the Molecular Dimensions and Oligomerization of Proteins at Liquid/Solid Interfaces

Ultrasonic atomization of the DNA solution for atomic force microscopy

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