Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system

Goswami, Mayank; Zhang, Peng-Fei; Pugh, Edward N.; Zawadzki, Robert J.

doi:10.1117/12.2213609

Cited by 3 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7(g) ], which has not been previously visualized in the mouse in vivo without contrast agent. 46 , 47 OCTA data sets can be used to produce very high contrast maps of retinal vasculature, as illustrated with a depth-color-coded projection of the inner retina vessels [ Fig. 7(h) ].…”

Section: Resultsmentioning

confidence: 99%

Temporal speckle-averaging of optical coherence tomography volumes for in-vivo cellular resolution neuronal and vascular retinal imaging

et al. 2019

Self Cite

View full text Add to dashboard Cite

It has been recently demonstrated that structures corresponding to the cell bodies of highly transparent cells in the retinal ganglion cell layer could be visualized noninvasively in the living human eye by optical coherence tomography (OCT) via temporal averaging. Inspired by this development, we explored the application of volumetric temporal averaging in mice, which are important models for studying human retinal diseases and therapeutic interventions. A general framework of temporal speckle-averaging (TSA) of OCT and optical coherence tomography angiography (OCTA) is presented and applied to mouse retinal volumetric data. Based on the image analysis, the eyes of mice under anesthesia exhibit only minor motions, corresponding to lateral displacements of a few micrometers and rotations of a fraction of 1 deg. Moreover, due to reduced eye movements under anesthesia, there is a negligible amount of motion artifacts within the volumes that need to be corrected to achieve volume coregistration. In addition, the relatively good optical quality of the mouse ocular media allows for cellular-resolution imaging without adaptive optics (AO), greatly simplifying the experimental system, making the proposed framework feasible for large studies. The TSA OCT and TSA OCTA results provide rich information about new structures previously not visualized in living mice with non-AO-OCT. The mechanism of TSA relies on improving signal-to-noise ratio as well as efficient suppression of speckle contrast due to temporal decorrelation of the speckle patterns, enabling full utilization of the high volumetric resolution offered by OCT and OCTA. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Resultsmentioning

confidence: 99%

Temporal speckle-averaging of optical coherence tomography volumes for in-vivo cellular resolution neuronal and vascular retinal imaging

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“… Simultaneous SLO and OCT imaging of a nude mouse with two fluorescence channels injected with fluorescein and nanoparticles tagged with rhodamine-X: (A) Image of ”green” (“GFP”) fluorescence channel; (B) Image of ”red” fluorescence channel; (C) Image of the ratio between (B) and (A); D Image of the retinal vasculature extracted from OCT (pv-OCT); (E) Image of the choroidal vasculature obtained from OCT. (F-J) x3 magnified images of the white dashed area from Figure 4 A-E respectively showing enhanced visualization of chorioretinal vasculature. ( Adapted from Goswami et al 2016 163 ). …”

Section: Figurementioning

confidence: 99%

“…Micrographs showing pv-OCT/SLO imaging showing images visualized at different layers such as nerve fiber layer (NFL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS), retinal pigment epithelium (RPE), and choroid, respectively. ( Adapted from Goswami et al 2016 163 ).…”

Section: Figurementioning

confidence: 99%

Non-invasive multimodal imaging of Diabetic Retinopathy: A survey on treatment methods and Nanotheranostics

Sadasivam¹,

Gopinath²,

Shakya³

et al. 2021

Nanotheranostics

Self Cite

View full text Add to dashboard Cite

Diabetes Retinopathy (DR) is one of the most prominent microvascular complications of diabetes. It is one of the pre-eminent causes for vision impairment followed by blindness among the working-age population worldwide. The de facto cause for DR remains challenging, despite several efforts made to unveil the mechanism underlying the pathology of DR. There is quite less availability of the low cost pre-emptive theranostic imaging tools in terms of in-depth resolution, due to the multiple factors involved in the etiology of DR. This review work comprehensively explores the various reports and research works on all perspectives of diabetic retinopathy (DR), and its mechanism. It also discusses various advanced non-destructive imaging modalities, current, and future treatment approaches. Further, the application of various nanoparticle-based drug delivery strategies used for the treatment of DR are also discussed. In a nutshell, the present review work bolsters the pursuit of the development of an advanced non-invasive optical imaging modal with a nano-theranostic approach for the future diagnosis and treatment of DR and its associated ocular complications.

show abstract

“…Recent progress in polarization-sensitive OCT (PS-OCT), a functional extension of OCT that is capable of visualizing and quantifying melanin concentrations at the retina enabled clinicians to precisely diagnose multiple stages of AMD and has been widely employed in the research environment [4]. Moreover, there have been studies that quantified lipofuscin from the retina with autofluorescence measurement [5,6], and visualization of CC has also been conducted using a mouse model [7]. However, none of the studies reported the development of a multi-modal system that could acquire images of melanin concentration, lipofuscin accumulation and CC visualization with a single imaging system.…”

Section: Introductionmentioning

confidence: 99%

Multi-modal functional sensorless adaptive optics for small animal retinal imaging

Song,

Miao,

Matsubara

et al. 2023

Optical Coherence Imaging Techniques and Imaging in Scattering Media V

View full text Add to dashboard Cite

The proposed preclinical study investigates pathological characteristics of retinal diseases such as age-related macular degeneration (AMD) with transgenic small animal models using a multi-modal functional small animal retinal imaging system. For characterizing the animal models, we visualize the melanin concentration, lipofuscin accumulation, and choriocapillaris using a single imaging system. The system implements polarization-sensitive optical coherence tomography (PS-OCT), fluorescence scanning laser ophthalmoscope (fSLO), and sensorless adaptive optics (SAO) for the visualizations of pathological features. As preliminary data, we acquired three different mice models and visualized the outer retinal thickness and melanin concentration. The newly developing system is expected to provide multilateral perspectives for further studies in AMD, enabling vision scientists to investigate the correlations between melanin, lipofuscin, and choriocapillaris for the root cause of AMD.

show abstract

Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system

Cited by 3 publications

References 14 publications

Temporal speckle-averaging of optical coherence tomography volumes for in-vivo cellular resolution neuronal and vascular retinal imaging

Temporal speckle-averaging of optical coherence tomography volumes for in-vivo cellular resolution neuronal and vascular retinal imaging

Non-invasive multimodal imaging of Diabetic Retinopathy: A survey on treatment methods and Nanotheranostics

Multi-modal functional sensorless adaptive optics for small animal retinal imaging

Contact Info

Product

Resources

About